Literature summary for 1.1.5.13 extracted from

Kalliri, E.; Mulrooney, S.B.; Hausinger, R.P.
Identification of Escherichia coli YgaF as an L-2-hydroxyglutarate oxidase (2008), J. Bacteriol., 190, 3793-3798.

Search for more literature for 1.1.5.13

Cloned(Commentary)

Cloned (Comment)	Organism
into the vector pET42b	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.095	-	L-2-hydroxyglutarate	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47640	-	theoretical value, verified by SDS-PAGE	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using a Ni-charged nitrilotriacetic acid-Sepharose 6 fast-flow column	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-2-hydroxyglutarate + oxidized o-phenylendiamine	experimental setup	Escherichia coli	alpha-ketoglutarate + reduced o-phenylendiamine	-	ir

Synonyms

Synonyms	Comment	Organism
YgaF	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.08	-	L-2-hydroxyglutarate	-	Escherichia coli

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Release 2023.1 (January 2023)