Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.5.14 extracted from

  • Kimata, S.; Mizuguchi, K.; Hattori, S.; Teshima, S.; Orita, Y.
    Evaluation of a new automated, enzymatic inulin assay using D-fructose dehydrogenase (2009), Clin. Exp. Nephrol., 13, 341-349.
    View publication on PubMed

Application

Application Comment Organism
biotechnology an automated, enzymatic insulin assay is developed. Principle: Fructose is produced by the action of inulinase on inulin present in the sample. The resulting fructose reacts with D-fructose dehydrogenase in the presence of the oxidized form of 1-methoxy-5-methylphenazinium methylsulfate (1-m-PMS) to produce the reduced form of 1-m-PMS. Reduced 1-m-PMS acts on dissolved oxygen to produce hydrogen peroxide, which, through the action of peroxidase, oxidatively condenses N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine and 4-aminoantipyrine to transform them into quinoneimine dye. The absorbance of the quinoneimine dye is measured spectrophotometrically to determine the concentration of inulin in the sample. The new enzymatic assay offers a more convenient and more accurate measurement of inulin and may be suitable for routine procedures by automated analyzers in clinical laboratories Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + oxidized 1-methoxy-5-methylphenazinium methylsulfate
-
Gluconobacter oxydans 5-dehydro-D-fructose + reduced 1-methoxy-5-methylphenazinium methylsulfate
-
?

Synonyms

Synonyms Comment Organism
D-fructose dehydrogenase
-
Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Gluconobacter oxydans