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Literature summary for 1.1.5.14 extracted from

  • Suzuki, Y.; Kano, K.; Shirai, O.; Kitazumi, Y.
    Diffusion-limited electrochemical D-fructose sensor based on direct electron transfer-type bioelectrocatalysis by a variant of D-fructose dehydrogenase at a porous gold microelectrode (2020), J. Electroanal. Chem., 877, 114651 .
No PubMed abstract available
Search for more literature for 1.1.5.14

Application

Application Comment Organism
food industry the enzyme can be used as biosensor to quantify D-fructose in commercial beverages and honey Gluconobacter japonicus

Protein Variants

Protein Variants Comment Organism
M450Q/DELTA1cFDH the direct electron transfer-type bioelectrocatalytic activity of the mutant is higher than that of recombinant native enzyme Gluconobacter japonicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Gluconobacter japonicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 gel filtration Gluconobacter japonicus

Organism

Organism UniProt Comment Textmining
Gluconobacter japonicus
-
-
-
Gluconobacter japonicus NBRC 3260
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + potassium ferricyanide
-
 Gluconobacter japonicus 5-dehydro-D-fructose + potassium ferrocyanide
-
 ?
D-fructose + potassium ferricyanide
-
 Gluconobacter japonicus NBRC 3260 5-dehydro-D-fructose + potassium ferrocyanide
-
 ?

Subunits

Subunits Comment Organism
heterotrimer 1 * 67000 + 1 * 51000 + 1 * 20000, calculated from amino acid sequence Gluconobacter japonicus

Synonyms

Synonyms Comment Organism
D-fructose dehydrogenase
-
 Gluconobacter japonicus
FDH
-
 Gluconobacter japonicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Gluconobacter japonicus
heme c
-
 Gluconobacter japonicus