KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
phenazine methosulfate | membrane-bound enzyme form | Acinetobacter calcoaceticus | |
1.9 | - |
phenazine methosulfate | soluble enzyme form | Acinetobacter calcoaceticus | |
4.2 | - |
D-glucose | - |
Acinetobacter calcoaceticus | |
24.5 | - |
D-glucose | soluble enzyme | Acinetobacter calcoaceticus | |
26.7 | - |
lactose | soluble enzyme | Acinetobacter calcoaceticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Acinetobacter calcoaceticus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, soluble enzyme SDS-PAGE | Acinetobacter calcoaceticus |
83000 | - |
x * 83000, soluble enzyme, SDS-PAGE | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
soluble enzyme and membrane-bound enzyme | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenol-indophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
D-glucose + phenazine methosulfate | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, soluble enzyme SDS-PAGE | Acinetobacter calcoaceticus |
? | x * 83000, soluble enzyme, SDS-PAGE | Acinetobacter calcoaceticus |
dimer | - |
Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
soluble enzyme | Acinetobacter calcoaceticus |
8.5 | - |
membrane-bound enzyme | Acinetobacter calcoaceticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | pH 7.5: about 40% of maximal activity, pH 9.5: about 85% of maximal activity, membrane-bound enzyme, reaction with phenazine methosulfate, 2,6-dichlorophenol indophenol and pyrroloquinoline quinone | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | prosthetic group | Acinetobacter calcoaceticus |