Application | Comment | Organism |
---|---|---|
biotechnology | engineering of the soluble enzyme GDH-B to enable the electron transfer to the electrode in absence of artificial electron mediator by mimicking the domain structure of the quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni, which is composed of a PQQ-containing catalytic domain and a cytochrome c domain | Acinetobacter calcoaceticus |
diagnostics | enzyme is industrially used as glucose sensor with high catalytic activity and insensitivity to oxygen | Acinetobacter calcoaceticus |
Cloned (Comment) | Organism |
---|---|
gene gdhB, expression of the enzyme GDH-B as fusion protein C-terminally fused to cytochrome c domain from Comamonas testosteroni in Escherichia coli DH5alpha | Acinetobacter calcoaceticus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the recombinant cytochrome c-fusion protein shows a highly increased sensitivity when immobilized to the electrode as D-glucose sensor compared to the wild-type enzyme, overview | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | D-glucose | recombinant cytochrome c-fusion protein, pH 7.0 | Acinetobacter calcoaceticus | |
15 | - |
maltose | recombinant cytochrome c-fusion protein, pH 7.0 | Acinetobacter calcoaceticus | |
19 | - |
lactose | recombinant cytochrome c-fusion protein, pH 7.0 | Acinetobacter calcoaceticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Acinetobacter calcoaceticus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for structure stabilization of the holoform | Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, recombinant cytochrome c-fusion protein, SDS-PAGE | Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ubiquinone | Acinetobacter calcoaceticus | - |
D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | Acinetobacter calcoaceticus LMD79.41 | - |
D-glucono-1,5-lactone + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Acinetobacter calcoaceticus LMD79.41 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant cytochrome c fusion protein from Escherichia coli | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus LMD79.41 | D-glucono-1,5-lactone + ? | - |
? | |
D-glucose + ubiquinone | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | - |
Acinetobacter calcoaceticus LMD79.41 | D-glucono-1,5-lactone + ubiquinol | - |
? | |
lactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
lactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus LMD79.41 | ? | - |
? | |
maltose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
maltose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus LMD79.41 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, recombinant cytochrome c-fusion protein, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms | Comment | Organism |
---|---|---|
GDH-B | - |
Acinetobacter calcoaceticus |
PQQ glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3178 | - |
D-glucose | recombinant cytochrome c-fusion protein, pH 7.0 | Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | i.e. PQQ, dependent on | Acinetobacter calcoaceticus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Acinetobacter calcoaceticus | calculated, recombinant cytochrome c-fusion protein | 4.7 | 4.6 |
Acinetobacter calcoaceticus | calculated, wild-type GDH-B | 4.7 | 4.6 |