Application | Comment | Organism |
---|---|---|
biotechnology | engineering PQQ glucose dehydrogenase with improved substrate specificity | Acinetobacter calcoaceticus |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant isozyme PQQGDH-B | Acinetobacter calcoaceticus |
Protein Variants | Comment | Organism |
---|---|---|
D167A | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167C | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167E | site-directed mutagenesis, substrate binding residue mutation, slightly reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167E/N452T | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167G | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167H | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167K | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167N | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167Q | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167R | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167S | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167V | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167W | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
D167Y | site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
H168C | site-directed mutagenesis, catalytic residue mutation, highly reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
H168Q | site-directed mutagenesis, catalytic residue mutation, nearly inactive mutant | Acinetobacter calcoaceticus |
K166E | site-directed mutagenesis, substrate binding residue mutation, altered substrate specificty compared to the wild-type enzyme | Acinetobacter calcoaceticus |
K166G | site-directed mutagenesis, substrate binding residue mutation, altered substrate specificty compared to the wild-type enzyme | Acinetobacter calcoaceticus |
K166I | site-directed mutagenesis, substrate binding residue mutation, altered substrate specificty compared to the wild-type enzyme | Acinetobacter calcoaceticus |
additional information | engineering PQQ glucose dehydrogenase with improved substrate specificity | Acinetobacter calcoaceticus |
N452T | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Acinetobacter calcoaceticus |
Q169E | site-directed mutagenesis, substrate binding residue mutation, altered substrate specificty compared to the wild-type enzyme | Acinetobacter calcoaceticus |
Q169K | site-directed mutagenesis, substrate binding residue mutation, altered substrate specificty compared to the wild-type enzyme | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
D-galactose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
5.3 | - |
D-galactose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
12.5 | - |
D-glucose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
14 | - |
cellobiose | recombinant wild-type isozyme PQQGDH-B and mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
16 | - |
cellobiose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
16 | - |
maltose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
17 | - |
cellobiose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
18.9 | - |
lactose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
25 | - |
D-glucose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
26 | - |
maltose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
27.6 | - |
3-O-methyl-D-glucose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
28.7 | - |
3-O-methyl-D-glucose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
33.6 | - |
lactose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
35.5 | - |
allose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
38.7 | - |
allose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
46.5 | - |
maltose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
48 | - |
D-glucose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
55 | - |
D-glucose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
55 | - |
lactose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
77 | - |
lactose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
99 | - |
3-O-methyl-D-glucose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
145 | - |
D-galactose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
154 | - |
D-glucose | recombinant mutant H168Q, pH 7.0 | Acinetobacter calcoaceticus | |
156 | - |
maltose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
182 | - |
allose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
193 | - |
D-glucose | recombinant mutant H168C, pH 7.0 | Acinetobacter calcoaceticus | |
198 | - |
3-O-methyl-D-glucose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
199 | - |
allose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | isozyme PQQGDH-B | Acinetobacter calcoaceticus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | P13650 | isozyme PQQGDH-B | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | 2-deoxy-D-glucono-1,5-lactone + ? | - |
? | |
3-O-methyl-D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + ? | - |
? | |
allose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
cellobiose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
D-galactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + ? | - |
? | |
D-glucose + 2,6-dichlorophenolindolphenol | best substrate | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
D-glucose + ubiquinone | best substrate | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-mannose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
D-xylose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-xylono-1,5-lactone + ? | - |
? | |
lactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
maltose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
additional information | substrate specificities of recombinant wild-type and mutant enzymes, overview | Acinetobacter calcoaceticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PQQ glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
PQQGDH-B | - |
Acinetobacter calcoaceticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
thermal stability of wild-type and mutant isozymes PQQGDH-B, overview | Acinetobacter calcoaceticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
D-glucose | recombinant mutant H168Q, pH 7.0 | Acinetobacter calcoaceticus | |
2.5 | - |
D-glucose | recombinant mutant H168C, pH 7.0 | Acinetobacter calcoaceticus | |
65 | - |
maltose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
72 | - |
D-galactose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
73 | - |
allose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
89 | - |
D-galactose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
167 | - |
lactose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
215 | - |
3-O-methyl-D-glucose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
226 | - |
cellobiose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
232 | - |
D-galactose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
436 | - |
maltose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
478 | - |
lactose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
541 | - |
3-O-methyl-D-glucose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
558 | - |
allose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
949 | - |
allose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1002 | - |
maltose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1038 | - |
lactose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1060 | - |
cellobiose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
1073 | - |
cellobiose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1193 | - |
D-glucose | recombinant mutant D167E/N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1253 | - |
3-O-methyl-D-glucose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1355 | - |
cellobiose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
1659 | - |
lactose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
1724 | - |
D-glucose | recombinant mutant D167E, pH 7.0 | Acinetobacter calcoaceticus | |
1791 | - |
D-glucose | recombinant mutant N452T, pH 7.0 | Acinetobacter calcoaceticus | |
1930 | - |
maltose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
2509 | - |
allose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
3011 | - |
3-O-methyl-D-glucose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus | |
3860 | - |
D-glucose | recombinant wild-type isozyme PQQGDH-B, pH 7.0 | Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | i.e. PQQ, dependent on | Acinetobacter calcoaceticus |