Cloned (Comment) | Organism |
---|---|
into the pET M-11 vector for transformation of DH5alpha and BL21DE3 cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
apo- and holoenzyme at a resolution of 1.5 A | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
150 | - |
maltotriose | - |
Escherichia coli | |
170 | - |
maltose | - |
Escherichia coli | |
400 | - |
D-glucose | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
determined by SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ubiquinone | Escherichia coli | - |
D-glucono-1,5-lactone + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Ni-NTA column, the hexahistidine tag is removed by cleavage with tobacco edge virus protease, cleaved protein is purified by gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ubiquinone | - |
Escherichia coli | D-glucono-1,5-lactone + ubiquinol | - |
? | |
maltose + ubiquinone | - |
Escherichia coli | ? + ubiquinol | - |
? | |
maltotriose + 2,6-dichlorophenolindophenol | - |
Escherichia coli | ? | - |
r |
Synonyms | Comment | Organism |
---|---|---|
aldose sugar dehydrogenase | - |
Escherichia coli |
Asd | - |
Escherichia coli |
quinoprotein glucose dehydrogenase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2270 | - |
maltotriose | - |
Escherichia coli | |
2870 | - |
maltose | - |
Escherichia coli | |
3360 | - |
D-glucose | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
activity assay | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | PQQ | Escherichia coli |