Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.5.2 extracted from

  • Southall, S.M.; Doel, J.J.; Richardson, D.J.; Oubrie, A.
    Soluble aldose sugar dehydrogenase from Escherichia coli: a highly exposed active site conferring broad substrate specificity (2006), J. Biol. Chem., 281, 30650-30659.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pET M-11 vector for transformation of DH5alpha and BL21DE3 cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
apo- and holoenzyme at a resolution of 1.5 A Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
150
-
maltotriose
-
Escherichia coli
170
-
maltose
-
Escherichia coli
400
-
D-glucose
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
determined by SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ubiquinone Escherichia coli
-
D-glucono-1,5-lactone + ubiquinol
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Ni-NTA column, the hexahistidine tag is removed by cleavage with tobacco edge virus protease, cleaved protein is purified by gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + ubiquinone
-
Escherichia coli D-glucono-1,5-lactone + ubiquinol
-
?
maltose + ubiquinone
-
Escherichia coli ? + ubiquinol
-
?
maltotriose + 2,6-dichlorophenolindophenol
-
Escherichia coli ?
-
r

Synonyms

Synonyms Comment Organism
aldose sugar dehydrogenase
-
Escherichia coli
Asd
-
Escherichia coli
quinoprotein glucose dehydrogenase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
activity assay Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2270
-
maltotriose
-
Escherichia coli
2870
-
maltose
-
Escherichia coli
3360
-
D-glucose
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
activity assay Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone PQQ Escherichia coli