Cloned (Comment) | Organism |
---|---|
mutants are expressed in Escherichia coli YU423 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D354N | mutant retains a conformation almost unaltered compared to the wild type mGDH and strongly reduced activity | Escherichia coli |
D466E | mutant shows no significant difference in molecular structure from that of the wild type mGDH but has remarkably reduced content of bound ubiquinone and less than 0.04% activity compared to the wild type enzyme | Escherichia coli |
D466N | mutant shows no significant difference in molecular structure from that of the wild type mGDH but has remarkably reduced content of bound ubiquinone and less than 0.04% activity compared to the wild type enzyme | Escherichia coli |
K493A | mutant shows no significant difference in molecular structure from that of the wild type mGDH but has remarkably reduced content of bound ubiquinone and less than 0.04% activity compared to the wild type enzyme | Escherichia coli |
K493R | mutant retains a conformation almost unaltered compared to the wild type mGDH, the rate of ubiquinone to pyrroloquinoline electron transfer is about 4fold slower than that of the wild type enzyme, shows less than 0.04% activity compared to the wild type enzyme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli YU423 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography and hydroxyapatite column chromatography | Escherichia coli |
using two column chromatographies of DEAE-Toyopearl and ceramic hydroxyapatite | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + pyrroloquinoline quinone | - |
Escherichia coli | D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
D-glucose + pyrroloquinoline quinone | - |
Escherichia coli YU423 | D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
phenazine methosulfate + 2,6-dichlorophenol indophenol | - |
Escherichia coli | ? | - |
? | |
phenazine methosulfate + 2,6-dichlorophenol indophenol | - |
Escherichia coli YU423 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucose dehydrogenase | - |
Escherichia coli |
membrane-bound glucose dehydrogenase | - |
Escherichia coli |
mGDH | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Escherichia coli | |
pyrroloquinoline quinone | coenzyme | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | enzymatic analysis of purified mGDH from cells defective in synthesis of ubiquinone and/or menaquinone reveal that quinone-free mGDH has very low levels of activity of glucose dehydrogenase and UQ2 reductase compared with those of ubiquinone-bearing mGDH, both activities are increased by reconstitution with ubuquinone1. mGDH utilizes both menaquinone and ubiquinone as a bound quinine. Bound menaquinone occurs in a fashion similar to that of bound ubiquinone in the mGDH molecule and functions as an electron acceptor from pyrrolo quinoline quinone | Escherichia coli |