Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells | Pyrobaculum aerophilum |
Crystallization (Comment) | Organism |
---|---|
apoform and pyrroloquinoline quinone-bound holoenzyme, sitting drop vapor diffusion method, using 1.5 M ammonium sulfate and 0.1 M Tris/HCl buffer (pH 8.4), at 25°C | Pyrobaculum aerophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methylhydrazine | - |
Pyrobaculum aerophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
390 | - |
D-galactose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
390 | - |
D-fructose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
500 | - |
D-xylose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
600 | - |
maltose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
680 | - |
D-glucose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
910 | - |
D-mannose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
gel filtration | Pyrobaculum aerophilum |
32000 | - |
1 * 32000, SDS-PAGE | Pyrobaculum aerophilum |
38452 | - |
1 * 38452, calculated from amino acid sequence | Pyrobaculum aerophilum |
40000 | - |
dynamic light scattering | Pyrobaculum aerophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + pyrroloquinoline quinone | Pyrobaculum aerophilum | - |
D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum aerophilum | Q8ZUN8 | - |
- |
Purification (Comment) | Organism |
---|---|
HiPrep 16/10 SP XL column chromatography | Pyrobaculum aerophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.77 | - |
purified enzyme,in 50 mM BisTris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-cellobiose + 2,6-dichloroindophenol | - |
Pyrobaculum aerophilum | D-cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-fructose + 2,6-dichloroindophenol | - |
Pyrobaculum aerophilum | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-galactose + 2,6-dichloroindophenol | - |
Pyrobaculum aerophilum | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichloroindophenol | - |
Pyrobaculum aerophilum | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + pyrroloquinoline quinone | - |
Pyrobaculum aerophilum | D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
D-mannose + 2,6-dichloroindophenol | - |
Pyrobaculum aerophilum | D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-ribose + 2,6-dichloroindophenol | - |
Pyrobaculum aerophilum | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichloroindophenol | - |
Pyrobaculum aerophilum | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-arabinose + 2,6-dichloroindophenol | - |
Pyrobaculum aerophilum | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + 2,6-dichloroindophenol | - |
Pyrobaculum aerophilum | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, SDS-PAGE | Pyrobaculum aerophilum |
monomer | 1 * 38452, calculated from amino acid sequence | Pyrobaculum aerophilum |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Pyrobaculum aerophilum |
quinoprotein aldose sugar dehydrogenase | - |
Pyrobaculum aerophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
above 75°C | Pyrobaculum aerophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
the enzyme is extremely thermostable, and the activity of the pyrroloquinoline quinone-bound holoenzyme is not lost after incubation at 100 °C for 10 min, while the apoenzyme retains full activity at up to 80°C under the same conditions | Pyrobaculum aerophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
maltose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
3.1 | - |
D-mannose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
3.2 | - |
D-xylose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
4.1 | - |
D-glucose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
4.3 | - |
D-galactose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
the optimum pH for D-glucose oxidation at 50°C is around pH 8.0 | Pyrobaculum aerophilum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | when heated at 70°C for 30 min, the apo- and holoenzymes do not lose activity at pHs in the ranges of 4-11 and 3-11, respectively | Pyrobaculum aerophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Pyrobaculum aerophilum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
400 | - |
D-fructose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
1700 | - |
D-ribose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
2900 | - |
D-cellobiose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
3400 | - |
D-mannose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
3900 | - |
maltose | in 50 mM Bis-ris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
4100 | - |
L-arabinose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
6100 | - |
D-glucose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
6500 | - |
D-xylose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum | |
11000 | - |
D-galactose | in 50 mM Bis-Tris propane (pH 8.0), at 50°C | Pyrobaculum aerophilum |