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Literature summary for 1.1.5.2 extracted from
- High-level production of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase in Escherichia coli (2012), Eng. Life Sci., 12, 574-582.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | the enzyme is attractive for application in glucose detection in clinic diagnosis and industrial bioprocess controls | Acinetobacter calcoaceticus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant high-level production of soluble His-tagged pyrroloquinoline quinone-dependent glucose dehydrogenase in Escherichia coli strain BL21 (DE3), 6fold improvement improvement of sPQQGDH expression in MMBL medium | Acinetobacter calcoaceticus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | isozyme mPQQGDH | Acinetobacter calcoaceticus | 16020 | - |
| additional information | there are two types of PQQGDH, the membrane-bound glucose dehydrogenase and the soluble glucose dehydrogenase | Acinetobacter calcoaceticus | - |
- |
| periplasm | soluble isozyme sPQQGDH | Acinetobacter calcoaceticus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | three ions per enzyme subunit | Acinetobacter calcoaceticus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + ubiquinone | Acinetobacter calcoaceticus | preferred substrate | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| D-glucose + ubiquinone | Acinetobacter calcoaceticus L.M.D. 79.41 | preferred substrate | D-glucono-1,5-lactone + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
- |
- |
| Acinetobacter calcoaceticus L.M.D. 79.41 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble His-tagged enzyme 11.3fold from Escherichia coli strain BL21 (DE3)by nickel affinity chromatography | Acinetobacter calcoaceticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | effects of different carbon sources and glucose concentrations on the cell growth and sPQQGDH production, overview | Acinetobacter calcoaceticus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5811 | - |
purified recombinant His-tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + ubiquinone | preferred substrate | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| D-glucose + ubiquinone | preferred substrate, assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| D-glucose + ubiquinone | preferred substrate | Acinetobacter calcoaceticus L.M.D. 79.41 | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| D-glucose + ubiquinone | preferred substrate, assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol | Acinetobacter calcoaceticus L.M.D. 79.41 | D-glucono-1,5-lactone + ubiquinol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PQQGDH | - |
Acinetobacter calcoaceticus |
| pyrroloquinoline quinone-dependent glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Acinetobacter calcoaceticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
purified recombinant enzyme, 10 min, stable | Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | required for catalysis, one molecule per enzyme subunit | Acinetobacter calcoaceticus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the purified recombinant PQQGDH shows thermal stability and substrate specificity as the native enzyme | Acinetobacter calcoaceticus |
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Release 2023.1 (January 2023)
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