Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phospholipid | activates | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Sodium amytal | 1 mM, competitive with respect to phosphatidylethanolamine, noncompetitive with respect to FAD | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
ubiquinone-9 | - |
Pseudomonas putida | |
0.45 | - |
(S)-malate | - |
Pseudomonas putida | |
3 | - |
vitamin K3 | - |
Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | firmly bound to | Pseudomonas putida | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
Chester | - |
Pseudomonas putida Chester | - |
Chester | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-15°C, 24 h, 70% loss of activity | Pseudomonas putida |
0°C, 24 h, 50% loss of activity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + oxidized 2,6-dichlorophenol indophenol | - |
Pseudomonas putida | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
(S)-malate + oxidized 2,6-dichlorophenol indophenol | - |
Pseudomonas putida Chester | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
(S)-malate + ubiquinone-0 | - |
Pseudomonas putida | oxaloacetate + ubiquinol-0 | - |
? | |
(S)-malate + ubiquinone-0 | - |
Pseudomonas putida Chester | oxaloacetate + ubiquinol-0 | - |
? | |
(S)-malate + ubiquinone-6 | - |
Pseudomonas putida | oxaloacetate + ubiquinol-6 | - |
? | |
(S)-malate + ubiquinone-6 | - |
Pseudomonas putida Chester | oxaloacetate + ubiquinol-6 | - |
? | |
(S)-malate + ubiquinone-9 | - |
Pseudomonas putida | oxaloacetate + ubiquinol-9 | - |
? | |
(S)-malate + ubiquinone-9 | - |
Pseudomonas putida Chester | oxaloacetate + ubiquinol-9 | - |
? | |
(S)-malate + vitamin K1 | - |
Pseudomonas putida | oxaloacetate + reduced vitamin K1 | - |
? | |
(S)-malate + vitamin K1 | - |
Pseudomonas putida Chester | oxaloacetate + reduced vitamin K1 | - |
? | |
(S)-malate + vitamin K3 | - |
Pseudomonas putida | oxaloacetate + reduced vitamin K3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
24 h, 85% loss of activity | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | Km: 0.0004 mM | Pseudomonas putida |