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Literature summary for 1.1.5.5 extracted from
- PQQ-dependent alcohol dehydrogenase (QEDH) of Pseudomonas aeruginosa is involved in catabolism of acyclic terpenes (2010), J. Basic Microbiol., 50, 119-124.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene exaA, expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) pLysS RARE | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
ethanol | pH 9.0, 30°C | Pseudomonas aeruginosa |  |
| 0.0073 | - |
citronellol | pH 9.0, 30°C | Pseudomonas aeruginosa | |
| 0.11 | - |
geraniol | pH 9.0, 30°C | Pseudomonas aeruginosa | |
| 1.3 | - |
citral | pH 9.0, 30°C | Pseudomonas aeruginosa | |
| 2.4 | - |
citronellal | pH 9.0, 30°C | Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + ubiquinone | Pseudomonas aeruginosa | - |
acetaldehyde + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
gene PA1982 or exaA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS RARE by nickel affinity chromatography | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 1,3-butanediol | Pseudomonas aeruginosa |
| 1.3 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 1,3-propandiol | Pseudomonas aeruginosa |
| 3.1 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate geraniol | Pseudomonas aeruginosa |
| 3.3 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate citronellal | Pseudomonas aeruginosa |
| 3.8 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate citral | Pseudomonas aeruginosa |
| 4 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 3-methyl-1--butanol | Pseudomonas aeruginosa |
| 4.4 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-octanol in DMSO | Pseudomonas aeruginosa |
| 4.4 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 2-propanol | Pseudomonas aeruginosa |
| 4.5 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-hexanol in DMSO | Pseudomonas aeruginosa |
| 5.4 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 2-butanol | Pseudomonas aeruginosa |
| 5.6 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-octanol in H2O | Pseudomonas aeruginosa |
| 6.3 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate citronellol | Pseudomonas aeruginosa |
| 7.5 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-butanol | Pseudomonas aeruginosa |
| 7.7 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-propanol | Pseudomonas aeruginosa |
| 7.9 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-hexanol in H2O | Pseudomonas aeruginosa |
| 8.2 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-pentanol | Pseudomonas aeruginosa |
| 8.5 | - |
purified recombinant enzyme, pH 9.0, 30°C, substrate ethanol | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-butandiol + ubiquinone | very low activity, 0.94% of the activity with ethanol | Pseudomonas aeruginosa | ? + ubiquinol | - |
? | |
| 1,3-propandiol + ubiquinone | 15% of the activity with ethanol | Pseudomonas aeruginosa | ? + ubiquinol | - |
? | |
| 1-butanol + ubiquinone | 88% of the activity with ethanol | Pseudomonas aeruginosa | butanal + ubiquinol | - |
? | |
| 1-hexanol + ubiquinone | 93% of the activity with ethanol | Pseudomonas aeruginosa | hexanal + ubiquinol | - |
? | |
| 1-octanol + ubiquinone | 66% of the activity with ethanol | Pseudomonas aeruginosa | octanal + ubiquinol | - |
? | |
| 1-pentanol + ubiquinone | 97% of the activity with ethanol | Pseudomonas aeruginosa | pentanal + ubiquinol | - |
? | |
| 1-propanol + ubiquinone | 90% of the activity with ethanol | Pseudomonas aeruginosa | propanal + ubiquinol | - |
? | |
| 2-butanol + ubiquinone | 64% of the activity with ethanol | Pseudomonas aeruginosa | 2-butanone + ubiquinol | - |
? | |
| 2-propanol + ubiquinone | 51% of the activity with ethanol | Pseudomonas aeruginosa | acetone + ubiquinol | - |
? | |
| 3-methyl-1-butanol + ubiquinone | 48% of the activity with ethanol | Pseudomonas aeruginosa | 3-methyl-1-butanal + ubiquinol | - |
? | |
| citral + ubiquinol | 39% of the activity with ethanol | Pseudomonas aeruginosa | ? + ubiquinone | - |
? | |
| citronellal + ubiquinol | 45% of the activity with ethanol | Pseudomonas aeruginosa | citronellol + ubiquinone | - |
? | |
| citronellol + ubiquinone | 74% of the activity with ethanol | Pseudomonas aeruginosa | citronellal + ubiquinol | - |
? | |
| ethanol + ubiquinone | - |
Pseudomonas aeruginosa | acetaldehyde + ubiquinol | - |
? | |
| ethanol + ubiquinone | best substrate | Pseudomonas aeruginosa | acetaldehyde + ubiquinol | - |
? | |
| geraniol + ubiquinone | 37% of the activity with ethanol | Pseudomonas aeruginosa | geranial + ubiquinol | - |
? | |
| additional information | substrate specificity, assayed with dichlorophenolindophenol and phenazinemethosulfate as electron acceptors, overview | Pseudomonas aeruginosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PQQ-dependent alcohol dehydrogenase | - |
Pseudomonas aeruginosa |
| PQQ-dependent ethanol dehydrogenase | - |
Pseudomonas aeruginosa |
| QEDH | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | dependent on, enzyme bound | Pseudomonas aeruginosa | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | inactivation of PA1982 by insertion mutagenesis results in inability of the mutant to utilise ethanol and in reduced growth on geraniol. Growth on ethanol is restored by transferring an intact copy of the PA1982 gene into the mutant | Pseudomonas aeruginosa |
| physiological function | the PQQ-dependent alcohol dehydrogenase of Pseudomonas aeruginosa functions in ethanol metabolism and is involved in catabolism of acyclic terpenes, overview | Pseudomonas aeruginosa |
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