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Literature summary for 1.1.5.5 extracted from
- Effect of amines as activators on the alcohol-oxidizing activity of pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase (2014), Biosci. Biotechnol. Biochem., 78, 1195-1198.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ammonia | - |
Pseudomonas putida |  |
| Butylamine | - |
Pseudomonas putida | |
| Dimethylamine | - |
Pseudomonas putida | |
| dipropylamine | - |
Pseudomonas putida | |
| ethylamine | - |
Pseudomonas putida | |
| heptylamine | - |
Pseudomonas putida | |
| Hexylamine | second best activating amine | Pseudomonas putida | |
| Isobutylamine | - |
Pseudomonas putida | |
| methylamine | - |
Pseudomonas putida | |
| octylamine | - |
Pseudomonas putida | |
| pentylamine | best activating amine | Pseudomonas putida | |
| Propylamine | - |
Pseudomonas putida | |
| tert-Butylamine | - |
Pseudomonas putida | |
| triethylamine | - |
Pseudomonas putida | |
| trimethylamine | - |
Pseudomonas putida | |
| tripropylamine | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
ethanol | in the presence of pentylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
| 0.21 | - |
ethanol | in the presence of ethylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
| 0.35 | - |
1-propanol | in the presence of pentylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
| 0.43 | - |
1-propanol | in the presence of ethylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida KT 2240 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-propanol + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | propionaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1-propanol + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida KT 2240 | propionaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| ethanol + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | acetaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
| ethanol + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida KT 2240 | acetaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PQQ-ADH | - |
Pseudomonas putida |
| pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase | - |
Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 24.9 | - |
ethanol | in the presence of ethylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
| 32.6 | - |
1-propanol | in the presence of ethylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
| 56.6 | - |
ethanol | in the presence of pentylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
| 73.1 | - |
1-propanol | in the presence of pentylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 73 | - |
1-propanol | in the presence of ethylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
| 115 | - |
ethanol | in the presence of ethylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
| 209 | - |
1-propanol | in the presence of pentylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
| 395 | - |
ethanol | in the presence of pentylamine, at pH 9.0 and 25°C | Pseudomonas putida | |
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