Any feedback?
Literature summary for 1.1.5.9 extracted from
- Subunit Analyses of a Novel Thermostable Glucose Dehydrogenase Showing Different Temperature Properties According to Its Quaternary Structure (1999), Appl. Biochem. Biotechnol., 77-79, 325-335.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Urea | urea causes a dose-dependent but reversible inhibition by dissoziation of the hetero-oligomeric enzyme comparable with the effect of heat-treatment, 3.5 M urea reduces GDH activity by 20%, 5 M urea by 80% and 8 M urea by ca. 90% | Burkholderia cepacia |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
D-glucose | at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia | |
| 1.4 | - |
phenazine methosulfate | at 40°C | Burkholderia cepacia | |
| 2.5 | - |
D-glucose | at 40°C | Burkholderia cepacia | |
| 2.7 | - |
phenazine methosulfate | at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses | Burkholderia cepacia |
| 67000 | - |
SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses | Burkholderia cepacia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
bacterial strain SM4 is used, isolated from a soil sample near a Japanese hot spring | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
290 micromol/min/mg Vmax of oxidation of glucose at 40°C | Burkholderia cepacia |
| additional information | - |
4.3 micromol/min/mg Vmax of oxidation of phenazine methosulfate at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia |
| additional information | - |
470 micromol/min/mg Vmax of oxidation of phenazine methosulfate at 40°C | Burkholderia cepacia |
| additional information | - |
66 micromol/min/mg Vmax of oxidation of glucose at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + 2,6-dichlorophenolindophenol | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + phenazine methosulfate | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heteromer | SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses | Burkholderia cepacia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDH | - |
Burkholderia cepacia |
| glucose dehydrogenase | - |
Burkholderia cepacia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
after preincubation with 5.8 M urea for 30 min at 25°C | Burkholderia cepacia |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | after preincubation with 5.8 M urea for 30 min at 25°C | Burkholderia cepacia |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
