Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.9.1 extracted from

  • Groen, B.W.; van Kleef, M.A.G.; Duine, J.A.
    Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni (1986), Biochem. J., 234, 611-615.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.9.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 benzyl alcohol pH 7.0, 20°C Comamonas testosteroni
0.005
-
 1-butanol pH 7.0, 20°C Comamonas testosteroni
0.005
-
 1-Pentanol pH 7.0, 20°C Comamonas testosteroni
0.005
-
 1-Octanol pH 7.0, 20°C Comamonas testosteroni
0.04
-
 octanal pH 7.0, 20°C Comamonas testosteroni
0.06
-
 1-propanol pH 7.0, 20°C Comamonas testosteroni
0.1
-
 Butanal pH 7.0, 20°C Comamonas testosteroni
0.2
-
 6-aminohexan-1-ol pH 7.0, 20°C Comamonas testosteroni
0.28
-
 Butane-1,3-diol pH 7.0, 20°C Comamonas testosteroni
0.9
-
 acetaldehyde pH 7.0, 20°C Comamonas testosteroni
3
-
 formaldehyde pH 7.0, 20°C Comamonas testosteroni
5
-
 ethanol pH 7.0, 20°C Comamonas testosteroni

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ presence is essential for activity Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 1 * 65000, SDS-PAGE Comamonas testosteroni
70000
-
 gel filtration Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-

Storage Stability

Storage Stability Organism
-70°C, storage for prolonged periods without loss of activity Comamonas testosteroni
4°C, 0.15 M potassium phosphate buffer, pH 7.0, storage for routine purposes Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni butanal + ?
-
 r
1-octanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni octanal + ?
-
 r
1-pentanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni pentanal + ?
-
 r
1-propanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni propanal + ?
-
 r
6-aminohexan-1-ol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni 6-aminohexanal + ?
-
 r
acetaldehyde + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 r
benzyl alcohol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni benzaldehyde + ?
-
 r
butanal + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni 1-butanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 r
butane-1,3-diol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni ?
-
 r
ethanol + cytochrome c 5% of the activity with Wurster's Blue. In presence of phenazine methosulfate, 60% of the activity with Wurster's Blue Comamonas testosteroni ethanal + reduced cytochrome c
-
 r
ethanol + ferricyanide 100% of the activity with Wurster's Blue Comamonas testosteroni ethanal + ferrocyanide
-
 r
ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine i.e. Wurster's Blue Comamonas testosteroni ethanal + ?
-
 ?
ethanol + oxidized 2,6-dichlorophenolindophenol 10% of the activity with Wurster's Blue. In presence of phenazine methosulfate, 75% of the activity with Wurster's Blue Comamonas testosteroni ethanal + reduced 2,6-dichlorophenolindophenol
-
 r
formaldehyde + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni methanol + ?
-
 r
octanal + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Comamonas testosteroni 1-octanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Comamonas testosteroni

Synonyms

Synonyms Comment Organism
quinohaemoprotein alcohol dehydrogenase
-
 Comamonas testosteroni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
heme heme group participates in enzymatic mechanism Comamonas testosteroni
pyrroloquinoline quinone
-
 Comamonas testosteroni