Data extracted from this reference:
Metals/Ions
Ca2+
holoenzyme contains equimolar amounts of pyrroloquinoline quinone, Ca2+ and covalently bound heme
Comamonas testosteroni
Molecular Weight [Da]
71000
PAGE
Comamonas testosteroni
73200
1 * 71000, SDS-PAGE, 1 * 73200, calculated
Comamonas testosteroni
Organism
Purification (Commentary)
from ethanol-grown cells
Comamonas testosteroni
Specific Activity [micromol/min/mg]
20.5
Comamonas testosteroni
Substrates and Products (Substrate)
n-butanol + ferricyanide
389960
Comamonas testosteroni
butanal + ferrocyanide
?
Subunits
monomer
1 * 71000, SDS-PAGE, 1 * 73200, calculated
Comamonas testosteroni
Cofactor
heme
holoenzyme contains equimolar amounts of pyrroloquinoline quinone, Ca2+ and covalently bound heme. Low-spin heme protein
Comamonas testosteroni
pyrroloquinoline quinone
holoenzyme contains equimolar amounts of pyrroloquinoline quinone, Ca2+ and covalently bound heme. Reconstitution of apoenzyme with pyrroloquinoline quinone analogues results in a decreased activity and enantioselectivity for the oxidation of chiral alcohols. Possession of the o-quinone or o-quinol moiety is not essential for binding but it is for activity
Comamonas testosteroni
Cofactor (protein specific)
heme
holoenzyme contains equimolar amounts of pyrroloquinoline quinone, Ca2+ and covalently bound heme. Low-spin heme protein
Comamonas testosteroni
pyrroloquinoline quinone
holoenzyme contains equimolar amounts of pyrroloquinoline quinone, Ca2+ and covalently bound heme. Reconstitution of apoenzyme with pyrroloquinoline quinone analogues results in a decreased activity and enantioselectivity for the oxidation of chiral alcohols. Possession of the o-quinone or o-quinol moiety is not essential for binding but it is for activity
Comamonas testosteroni
Metals/Ions (protein specific)
Ca2+
holoenzyme contains equimolar amounts of pyrroloquinoline quinone, Ca2+ and covalently bound heme
Comamonas testosteroni
Molecular Weight [Da] (protein specific)
71000
PAGE
Comamonas testosteroni
73200
1 * 71000, SDS-PAGE, 1 * 73200, calculated
Comamonas testosteroni
Purification (Commentary) (protein specific)
from ethanol-grown cells
Comamonas testosteroni
Specific Activity [micromol/min/mg] (protein specific)
20.5
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
n-butanol + ferricyanide
389960
Comamonas testosteroni
butanal + ferrocyanide
?
Subunits (protein specific)
monomer
1 * 71000, SDS-PAGE, 1 * 73200, calculated
Comamonas testosteroni
Other publictions for EC 1.1.9.1
654868
Jongejan
Deuterium isotope effect on en ...
Comamonas testosteroni
Biochim. Biophys. Acta
1647
297-302
2003
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1
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1
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1
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1
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1
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2
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1
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-
-
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-
656080
Oubrie
Crystal structure of quinohemo ...
Comamonas testosteroni
J. Biol. Chem.
277
3727-3732
2002
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-
1
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1
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1
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678768
Toyama
Azurin involved in alcohol oxi ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
65
1617-1626
2001
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-
1
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2
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1
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1
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1
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701480
Oubrie
Crystallization of quinohemopr ...
Comamonas testosteroni
Acta Crystallogr. Sect. D
D57
1732-1734
2001
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1
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1
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1
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1
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1
1
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-
-
-
701774
Somers
-
Enantioselective oxidation of ...
Comamonas testosteroni
Appl. Biochem. Biotechnol.
75
151-161
1999
-
1
-
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-
59
-
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1
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36
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1
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59
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36
-
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705187
Stigter
-
Enantioselective oxidation of ...
Comamonas testosteroni
J. Mol. Catal. B
2
291-297
1997
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-
-
-
-
-
-
21
-
-
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-
-
1
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-
-
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-
-
22
-
-
-
-
-
-
-
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-
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-
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-
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389956
De Jong
Characterization of the intera ...
Comamonas testosteroni
Biochemistry
34
9451-9458
1995
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-
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1
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2
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2
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-
-
-
-
-
-
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-
-
-
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-
-
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-
389960
De Jong
Quinohemoprotein ethanol dehyd ...
Comamonas testosteroni
Eur. J. Biochem.
230
899-905
1995
-
-
-
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
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-
1
-
1
1
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-
-
-
-
-
-
-
2
-
-
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-
-
-
2
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687362
Toyama
Three distinct quinoprotein al ...
Pseudomonas putida
J. Bacteriol.
177
2442-2450
1995
-
-
-
-
-
-
-
7
-
-
2
-
-
1
-
-
-
-
-
-
1
-
26
1
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
1
-
26
1
-
-
-
-
-
-
-
-
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1
1
-
-
-
703526
Geerlof
Description of the kinetic mec ...
Comamonas testosteroni
Eur. J. Biochem.
226
537-546
1994
-
-
-
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6
-
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1
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-
-
-
-
-
7
-
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-
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7
-
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-
-
-
-
-
-
-
-
-
6
-
-
-
-
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-
-
-
-
-
-
-
7
-
-
-
-
7
-
-
-
-
-
-
-
-
6
6
389953
Groen
Quinohaemoprotein alcohol dehy ...
Comamonas testosteroni
Biochem. J.
234
611-615
1986
-
-
-
-
-
-
-
12
-
1
2
-
-
1
-
-
-
-
-
-
-
2
15
1
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
12
-
1
2
-
-
-
-
-
-
-
-
2
15
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-