Data extracted from this reference:
KM Value [mM]
2.2
ethanol
pH 7.5, 25°C
Comamonas testosteroni
Organism
Substrates and Products (Substrate)
ethanol + ferricyanide
654868
Comamonas testosteroni
ethanal + ferrocyanide
?
rac-2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane + ferricyanide
i.e. solketal. Enantiomeric ratio is 30 for solketal and 6 for rac-2,2-dimethyl-4-[1,1-2H]hydroxymethyl-1,3-[5,5,4-2H]dioxolane, d5-solketal. Isotopic substitution affects the relative kinetic weights of the initial hydron/deuteron transfer from substrate to cofactor and the subsequent proton/deuteron shift in the cofactor-product complex
654868
Comamonas testosteroni
? + ferrocyanide
?
Synonyms
quinohemoprotein alcohol dehydrogenase
Comamonas testosteroni
Turnover Number [1/s]
17.5
ethanol
pH 7.5, 25°C
Comamonas testosteroni
KM Value [mM] (protein specific)
2.2
ethanol
pH 7.5, 25°C
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
ethanol + ferricyanide
654868
Comamonas testosteroni
ethanal + ferrocyanide
?
rac-2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane + ferricyanide
i.e. solketal. Enantiomeric ratio is 30 for solketal and 6 for rac-2,2-dimethyl-4-[1,1-2H]hydroxymethyl-1,3-[5,5,4-2H]dioxolane, d5-solketal. Isotopic substitution affects the relative kinetic weights of the initial hydron/deuteron transfer from substrate to cofactor and the subsequent proton/deuteron shift in the cofactor-product complex
654868
Comamonas testosteroni
? + ferrocyanide
?
Turnover Number [1/s] (protein specific)
17.5
ethanol
pH 7.5, 25°C
Comamonas testosteroni
Other publictions for EC 1.1.9.1
654868
Jongejan
Deuterium isotope effect on en ...
Comamonas testosteroni
Biochim. Biophys. Acta
1647
297-302
2003
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656080
Oubrie
Crystal structure of quinohemo ...
Comamonas testosteroni
J. Biol. Chem.
277
3727-3732
2002
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678768
Toyama
Azurin involved in alcohol oxi ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
65
1617-1626
2001
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701480
Oubrie
Crystallization of quinohemopr ...
Comamonas testosteroni
Acta Crystallogr. Sect. D
D57
1732-1734
2001
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701774
Somers
-
Enantioselective oxidation of ...
Comamonas testosteroni
Appl. Biochem. Biotechnol.
75
151-161
1999
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1
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59
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705187
Stigter
-
Enantioselective oxidation of ...
Comamonas testosteroni
J. Mol. Catal. B
2
291-297
1997
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21
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21
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22
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389956
De Jong
Characterization of the intera ...
Comamonas testosteroni
Biochemistry
34
9451-9458
1995
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389960
De Jong
Quinohemoprotein ethanol dehyd ...
Comamonas testosteroni
Eur. J. Biochem.
230
899-905
1995
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687362
Toyama
Three distinct quinoprotein al ...
Pseudomonas putida
J. Bacteriol.
177
2442-2450
1995
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7
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2
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26
1
2
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7
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26
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703526
Geerlof
Description of the kinetic mec ...
Comamonas testosteroni
Eur. J. Biochem.
226
537-546
1994
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6
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7
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6
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7
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7
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6
389953
Groen
Quinohaemoprotein alcohol dehy ...
Comamonas testosteroni
Biochem. J.
234
611-615
1986
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12
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