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show all sequences of 1.1.9.1

Enantioselective oxidation of secondary alcohols at a quinohemoprotein alcohol dehydrogenase electrode

Somers, W.A.C.; Stigter, E.C.A.; Van Hartingsveldt, W.; Van Der Lugt, J.P.; Appl. Biochem. Biotechnol. 75, 151-161 (1999)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
co-immobilization of enzyme with redox polymer poly(vinylpyridine) complex functionalized with osmium bis(bipyridine) chloride on an electrode. The enzyme electrode readily oxidizes primary alcohols and secondary alcohols with maximum current densities varying between 0.43 and 0.98 A per m2 depending on the substrate and the operation temperature. The enzyme electrode is enantioselective in the oxidation of secondary alcohols. A strong preference is observed for the (S)-2-alcohols, the enantioselectivity increases with increasing chain length. The enantiomeric ratio E increases from 13 for (R,S)-2-butanol to approximately 80 for (R,S)-2-heptanol and (R,S)-2-octanol
Comamonas testosteroni
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
1-butanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.005
-
1-Octanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.005
-
1-Pentanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.006
-
1-butanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.006
-
1-Octanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.006
-
1-Pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.04
-
(S)-2-heptanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.05
-
2-Octanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.05
-
(S)-2-octanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.06
-
1-propanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.06
-
(S)-2-heptanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.09
-
(S)-2-hexanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.1
-
(R)-solketal
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.11
-
(R)-solketal
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.24
-
5-methyl-2-pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.3
-
1,3-butanediol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.4
-
2-Pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.4
-
4-decanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.4
-
(R)-2-octanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.6
-
2-Pentanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.6
-
(R)-2-hexanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.7
-
4-heptanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.7
-
(R)-2-heptanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.75
-
4-methyl-2-pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.8
-
3-nonanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.8
-
Cyclohexanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.83
-
Cyclohexanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.9
-
3-methyl-2-pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.9
-
3-octanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.99
-
(S)-solketal
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
1
-
(S)-solketal
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
1.1
-
(R)-2-heptanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
1.2
-
(R)-2-hexanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
2.2
-
ethanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
2.9
-
3-Heptanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
4.3
-
3-Heptanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
4.5
-
(S)-2-butanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
4.5
-
(S)-glycidol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
4.8
-
(R)-glycidol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
5
-
(S)-2-butanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
5.3
-
3-Hexanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
6.3
-
3-Hexanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
8
-
2-butanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
12
-
2-butanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
22
-
2-propanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
28
-
3-Pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
28
-
(R)-2-butanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
30
-
2-propanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
30
-
3-Pentanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
30
-
(R)-2-butanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Comamonas testosteroni
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-2-butanol + ferricyanide
enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively
701774
Comamonas testosteroni
butan-2-one + ferrocyanide
-
-
-
?
(R)-2-heptanol + ferricyanide
enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 5.5% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively
701774
Comamonas testosteroni
heptan-2-one + ferrocyanide
-
-
-
?
(R)-2-hexanol + ferrocyanide
enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively
701774
Comamonas testosteroni
hexan-2-one + ferricyanide
-
-
-
?
(R)-2-octanol + ferrocyanide
enzyme electrode, 8% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively
701774
Comamonas testosteroni
octan-2-one + ferricyanide
-
-
-
?
(R)-glycidol + ferrocyanide
enzyme electrode, 37% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no distinction
701774
Comamonas testosteroni
? + ferricyanide
-
-
-
?
(R)-solketal + ferrocyanide
enzyme electrode, 35% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively
701774
Comamonas testosteroni
? + ferricyanide
-
-
-
?
(S)-2-butanol + ferricyanide
enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 13% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively
701774
Comamonas testosteroni
butan-2-one + ferrocyanide
-
-
-
?
(S)-2-heptanol + ferricyanide
enzyme electrode, 70% of vmax with 1-butanol, soluble enzyme, 63% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively
701774
Comamonas testosteroni
heptan-2-one + ferrocyanide
-
-
-
?
(S)-2-hexanol + ferrocyanide
enzyme electrode, 74% of vmax with 1-butanol, soluble enzyme, 71% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively
701774
Comamonas testosteroni
hexan-2-one + ferricyanide
-
-
-
?
(S)-2-octanol + ferrocyanide
enzyme electrode, 82% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively
701774
Comamonas testosteroni
octan-2-one + ferricyanide
-
-
-
?
(S)-glycidol + ferrocyanide
enzyme electrode, 30% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no disticnction
701774
Comamonas testosteroni
? + ferricyanide
-
-
-
?
(S)-solketal + ferrocyanide
enzyme electrode, 9% of vmax with 1-butanol, 0.7% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively
701774
Comamonas testosteroni
? + ferricyanide
-
-
-
?
1,3-butanediol + ferricyanide
enzyme electrode, 91% of vmax with 1-butanol
701774
Comamonas testosteroni
? + ferrocyanide
-
-
-
?
1-butanol + ferricyanide
maximum velocity rate for both enzyme electrode and soluble enzyme
701774
Comamonas testosteroni
butanal + ferrocyanide
-
-
-
?
1-octanol + ferrocyanide
enzyme electrode, 95% of vmax with 1-butanol, soluble enzyme, 95% of vmax with 1-butanol
701774
Comamonas testosteroni
octanal + ferricyanide
-
-
-
?
1-pentanol + ferrocyanide
enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 100% of vmax with 1-butanol
701774
Comamonas testosteroni
pentanal + ferricyanide
-
-
-
?
1-propanol + ferricyanide
enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 90% of vmax with 1-butanol
701774
Comamonas testosteroni
propanal + ferrocyanide
-
-
-
?
2-butanol + ferrocyanide
enzyme electrode, 17% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol
701774
Comamonas testosteroni
butanone + ferricyanide
-
-
-
?
2-octanol + ferrocyanide
enzyme electrode, 84% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol
701774
Comamonas testosteroni
octan-2-one + ferricyanide
-
-
-
?
2-pentanol + ferrocyanide
enzyme electrode, 40% of vmax with 1-butanol, soluble enzyme, 34% of vmax with 1-butanol
701774
Comamonas testosteroni
pentan-2-one + ferricyanide
-
-
-
?
2-propanol + ferrocyanide
enzyme electrode, 9% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol
701774
Comamonas testosteroni
acetone + ferricyanide
-
-
-
?
3-heptanol + ferrocyanide
enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 1% of vmax with 1-butanol
701774
Comamonas testosteroni
heptan-3-one + ferricyanide
-
-
-
?
3-hexanol + ferrocyanide
enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol
701774
Comamonas testosteroni
hexan-3-one + ferricyanide
-
-
-
?
3-methyl-2-pentanol + ferricyanide
enzyme electrode, 3.7% of vmax with 1-butanol
701774
Comamonas testosteroni
3-methylpentan-2-one + ferrocyanide
-
-
-
?
3-methyl-2-pentanol + ferricyanide
enzyme electrode, 9.3% of vmax with 1-butanol
701774
Comamonas testosteroni
3-methylpentan-2-one + ferrocyanide
-
-
-
?
3-nonanol + ferricyanide
enzyme electrode, 4.7% of vmax with 1-butanol, soluble enzyme, 4.5% of vmax with 1-butanol
701774
Comamonas testosteroni
nonan-3-one + ferrocyanide
-
-
-
?
3-octanol + ferrocyanide
enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol
701774
Comamonas testosteroni
octan-3-one + ferricyanide
-
-
-
?
3-pentanol + ferrocyanide
enzyme electrode, 1% of vmax with 1-butanol, soluble enzyme, 1.4% of vmax with 1-butanol
701774
Comamonas testosteroni
pentan-3-one + ferricyanide
-
-
-
?
4-decanol + ferricyanide
enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 3.6% of vmax with 1-butanol
701774
Comamonas testosteroni
decan-4-one + ferrocyanide
-
-
-
?
4-heptanol + ferricyanide
enzyme electrode, 9.3% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol
701774
Comamonas testosteroni
heptan-4-one + ferrocyanide
-
-
-
?
4-methyl-2-pentanol + ferricyanide
enzyme electrode, 5.6% of vmax with 1-butanol
701774
Comamonas testosteroni
4-methylpentan-2-one + ferrocyanide
-
-
-
?
cyclohexanol + ferricyanide
enzyme electrode, 11% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol
701774
Comamonas testosteroni
cyclohexanone + ferrocyanide
-
-
-
?
ethanol + ferricyanide
86% of vmax with 1-butanol, soluble enzyme, 75% of vmax with 1-butanol
701774
Comamonas testosteroni
ethanal + ferrocyanide
-
-
-
?
additional information
no substrate: D-glucose, D-galactose, D-fructose, Alpha-D-methylglucoside, mannitol
701774
Comamonas testosteroni
?
-
-
-
-
additional information
The affinity of the enzyme for aliphatic alcohols increases with the chain length of the substrate. The same property is observed for secondary alcohols in the series 2-propanol to 2-octanol
701774
Comamonas testosteroni
?
-
-
-
-
Application (protein specific)
Application
Commentary
Organism
biotechnology
co-immobilization of enzyme with redox polymer poly(vinylpyridine) complex functionalized with osmium bis(bipyridine) chloride on an electrode. The enzyme electrode readily oxidizes primary alcohols and secondary alcohols with maximum current densities varying between 0.43 and 0.98 A per m2 depending on the substrate and the operation temperature. The enzyme electrode is enantioselective in the oxidation of secondary alcohols. A strong preference is observed for the (S)-2-alcohols, the enantioselectivity increases with increasing chain length. The enantiomeric ratio E increases from 13 for (R,S)-2-butanol to approximately 80 for (R,S)-2-heptanol and (R,S)-2-octanol
Comamonas testosteroni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
1-butanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.005
-
1-Octanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.005
-
1-Pentanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.006
-
1-butanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.006
-
1-Octanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.006
-
1-Pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.04
-
(S)-2-heptanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.05
-
2-Octanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.05
-
(S)-2-octanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.06
-
1-propanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.06
-
(S)-2-heptanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.09
-
(S)-2-hexanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.1
-
(R)-solketal
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.11
-
(R)-solketal
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.24
-
5-methyl-2-pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.3
-
1,3-butanediol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.4
-
2-Pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.4
-
4-decanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.4
-
(R)-2-octanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.6
-
2-Pentanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.6
-
(R)-2-hexanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.7
-
4-heptanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.7
-
(R)-2-heptanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.75
-
4-methyl-2-pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.8
-
3-nonanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.8
-
Cyclohexanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.83
-
Cyclohexanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.9
-
3-methyl-2-pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.9
-
3-octanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
0.99
-
(S)-solketal
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
1
-
(S)-solketal
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
1.1
-
(R)-2-heptanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
1.2
-
(R)-2-hexanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
2.2
-
ethanol
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
2.9
-
3-Heptanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
4.3
-
3-Heptanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
4.5
-
(S)-2-butanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
4.5
-
(S)-glycidol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
4.8
-
(R)-glycidol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
5
-
(S)-2-butanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
5.3
-
3-Hexanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
6.3
-
3-Hexanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
8
-
2-butanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
12
-
2-butanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
22
-
2-propanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
28
-
3-Pentanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
28
-
(R)-2-butanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
30
-
2-propanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
30
-
3-Pentanol
soluble enzyme, pH 7.7, presence of CaCl2
Comamonas testosteroni
30
-
(R)-2-butanol
enzyme electrode, pH 7.7, presence of CaCl2
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-2-butanol + ferricyanide
enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively
701774
Comamonas testosteroni
butan-2-one + ferrocyanide
-
-
-
?
(R)-2-heptanol + ferricyanide
enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 5.5% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively
701774
Comamonas testosteroni
heptan-2-one + ferrocyanide
-
-
-
?
(R)-2-hexanol + ferrocyanide
enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively
701774
Comamonas testosteroni
hexan-2-one + ferricyanide
-
-
-
?
(R)-2-octanol + ferrocyanide
enzyme electrode, 8% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively
701774
Comamonas testosteroni
octan-2-one + ferricyanide
-
-
-
?
(R)-glycidol + ferrocyanide
enzyme electrode, 37% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no distinction
701774
Comamonas testosteroni
? + ferricyanide
-
-
-
?
(R)-solketal + ferrocyanide
enzyme electrode, 35% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively
701774
Comamonas testosteroni
? + ferricyanide
-
-
-
?
(S)-2-butanol + ferricyanide
enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 13% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively
701774
Comamonas testosteroni
butan-2-one + ferrocyanide
-
-
-
?
(S)-2-heptanol + ferricyanide
enzyme electrode, 70% of vmax with 1-butanol, soluble enzyme, 63% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively
701774
Comamonas testosteroni
heptan-2-one + ferrocyanide
-
-
-
?
(S)-2-hexanol + ferrocyanide
enzyme electrode, 74% of vmax with 1-butanol, soluble enzyme, 71% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively
701774
Comamonas testosteroni
hexan-2-one + ferricyanide
-
-
-
?
(S)-2-octanol + ferrocyanide
enzyme electrode, 82% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively
701774
Comamonas testosteroni
octan-2-one + ferricyanide
-
-
-
?
(S)-glycidol + ferrocyanide
enzyme electrode, 30% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no disticnction
701774
Comamonas testosteroni
? + ferricyanide
-
-
-
?
(S)-solketal + ferrocyanide
enzyme electrode, 9% of vmax with 1-butanol, 0.7% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively
701774
Comamonas testosteroni
? + ferricyanide
-
-
-
?
1,3-butanediol + ferricyanide
enzyme electrode, 91% of vmax with 1-butanol
701774
Comamonas testosteroni
? + ferrocyanide
-
-
-
?
1-butanol + ferricyanide
maximum velocity rate for both enzyme electrode and soluble enzyme
701774
Comamonas testosteroni
butanal + ferrocyanide
-
-
-
?
1-octanol + ferrocyanide
enzyme electrode, 95% of vmax with 1-butanol, soluble enzyme, 95% of vmax with 1-butanol
701774
Comamonas testosteroni
octanal + ferricyanide
-
-
-
?
1-pentanol + ferrocyanide
enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 100% of vmax with 1-butanol
701774
Comamonas testosteroni
pentanal + ferricyanide
-
-
-
?
1-propanol + ferricyanide
enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 90% of vmax with 1-butanol
701774
Comamonas testosteroni
propanal + ferrocyanide
-
-
-
?
2-butanol + ferrocyanide
enzyme electrode, 17% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol
701774
Comamonas testosteroni
butanone + ferricyanide
-
-
-
?
2-octanol + ferrocyanide
enzyme electrode, 84% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol
701774
Comamonas testosteroni
octan-2-one + ferricyanide
-
-
-
?
2-pentanol + ferrocyanide
enzyme electrode, 40% of vmax with 1-butanol, soluble enzyme, 34% of vmax with 1-butanol
701774
Comamonas testosteroni
pentan-2-one + ferricyanide
-
-
-
?
2-propanol + ferrocyanide
enzyme electrode, 9% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol
701774
Comamonas testosteroni
acetone + ferricyanide
-
-
-
?
3-heptanol + ferrocyanide
enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 1% of vmax with 1-butanol
701774
Comamonas testosteroni
heptan-3-one + ferricyanide
-
-
-
?
3-hexanol + ferrocyanide
enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol
701774
Comamonas testosteroni
hexan-3-one + ferricyanide
-
-
-
?
3-methyl-2-pentanol + ferricyanide
enzyme electrode, 3.7% of vmax with 1-butanol
701774
Comamonas testosteroni
3-methylpentan-2-one + ferrocyanide
-
-
-
?
3-methyl-2-pentanol + ferricyanide
enzyme electrode, 9.3% of vmax with 1-butanol
701774
Comamonas testosteroni
3-methylpentan-2-one + ferrocyanide
-
-
-
?
3-nonanol + ferricyanide
enzyme electrode, 4.7% of vmax with 1-butanol, soluble enzyme, 4.5% of vmax with 1-butanol
701774
Comamonas testosteroni
nonan-3-one + ferrocyanide
-
-
-
?
3-octanol + ferrocyanide
enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol
701774
Comamonas testosteroni
octan-3-one + ferricyanide
-
-
-
?
3-pentanol + ferrocyanide
enzyme electrode, 1% of vmax with 1-butanol, soluble enzyme, 1.4% of vmax with 1-butanol
701774
Comamonas testosteroni
pentan-3-one + ferricyanide
-
-
-
?
4-decanol + ferricyanide
enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 3.6% of vmax with 1-butanol
701774
Comamonas testosteroni
decan-4-one + ferrocyanide
-
-
-
?
4-heptanol + ferricyanide
enzyme electrode, 9.3% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol
701774
Comamonas testosteroni
heptan-4-one + ferrocyanide
-
-
-
?
4-methyl-2-pentanol + ferricyanide
enzyme electrode, 5.6% of vmax with 1-butanol
701774
Comamonas testosteroni
4-methylpentan-2-one + ferrocyanide
-
-
-
?
cyclohexanol + ferricyanide
enzyme electrode, 11% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol
701774
Comamonas testosteroni
cyclohexanone + ferrocyanide
-
-
-
?
ethanol + ferricyanide
86% of vmax with 1-butanol, soluble enzyme, 75% of vmax with 1-butanol
701774
Comamonas testosteroni
ethanal + ferrocyanide
-
-
-
?
additional information
no substrate: D-glucose, D-galactose, D-fructose, Alpha-D-methylglucoside, mannitol
701774
Comamonas testosteroni
?
-
-
-
-
additional information
The affinity of the enzyme for aliphatic alcohols increases with the chain length of the substrate. The same property is observed for secondary alcohols in the series 2-propanol to 2-octanol
701774
Comamonas testosteroni
?
-
-
-
-
Other publictions for EC 1.1.9.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
654868
Jongejan
Deuterium isotope effect on en ...
Comamonas testosteroni
Biochim. Biophys. Acta
1647
297-302
2003
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Oubrie
Crystal structure of quinohemo ...
Comamonas testosteroni
J. Biol. Chem.
277
3727-3732
2002
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678768
Toyama
Azurin involved in alcohol oxi ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
65
1617-1626
2001
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701480
Oubrie
Crystallization of quinohemopr ...
Comamonas testosteroni
Acta Crystallogr. Sect. D
D57
1732-1734
2001
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701774
Somers
-
Enantioselective oxidation of ...
Comamonas testosteroni
Appl. Biochem. Biotechnol.
75
151-161
1999
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50
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705187
Stigter
-
Enantioselective oxidation of ...
Comamonas testosteroni
J. Mol. Catal. B
2
291-297
1997
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389956
De Jong
Characterization of the intera ...
Comamonas testosteroni
Biochemistry
34
9451-9458
1995
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389960
De Jong
Quinohemoprotein ethanol dehyd ...
Comamonas testosteroni
Eur. J. Biochem.
230
899-905
1995
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687362
Toyama
Three distinct quinoprotein al ...
Pseudomonas putida
J. Bacteriol.
177
2442-2450
1995
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703526
Geerlof
Description of the kinetic mec ...
Comamonas testosteroni
Eur. J. Biochem.
226
537-546
1994
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389953
Groen
Quinohaemoprotein alcohol dehy ...
Comamonas testosteroni
Biochem. J.
234
611-615
1986
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