BRENDA - Enzyme Database show
show all sequences of 1.1.9.1

Enantioselective oxidation of secondary alcohols by quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni

Stigter, E.C.A.; van der Lugt, J.P.; Somers, W.A.C.; J. Mol. Catal. B 2, 291-297 (1997)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
1-Octanol
pH 7.5, 25°C
Comamonas testosteroni
0.005
-
1-Pentanol
pH 7.5, 25°C
Comamonas testosteroni
0.006
-
1-butanol
pH 7.5, 25°C
Comamonas testosteroni
0.05
-
2-Octanol
pH 7.5, 25°C
Comamonas testosteroni
0.06
-
1-propanol
pH 7.5, 25°C
Comamonas testosteroni
0.11
-
3-Methylcyclohexanol
pH 7.5, 25°C
Comamonas testosteroni
0.4
-
4-decanol
pH 7.5, 25°C
Comamonas testosteroni
0.6
-
2-Pentanol
pH 7.5, 25°C
Comamonas testosteroni
0.7
-
4-heptanol
pH 7.5, 25°C
Comamonas testosteroni
0.7
-
Ethanal
pH 7.5, 25°C
Comamonas testosteroni
0.8
-
3-nonanol
pH 7.5, 25°C
Comamonas testosteroni
0.83
-
Cyclohexanol
pH 7.5, 25°C
Comamonas testosteroni
0.9
-
3-octanol
pH 7.5, 25°C
Comamonas testosteroni
2.2
-
ethanol
pH 7.5, 25°C
Comamonas testosteroni
2.9
-
3-Heptanol
pH 7.5, 25°C
Comamonas testosteroni
5.3
-
3-Hexanol
pH 7.5, 25°C
Comamonas testosteroni
8
-
2-butanol
pH 7.5, 25°C
Comamonas testosteroni
30
-
2-propanol
pH 7.5, 25°C
Comamonas testosteroni
30
-
3-Pentanol
pH 7.5, 25°C
Comamonas testosteroni
50
-
1,3-cyclohexandiol
pH 7.5, 25°C
Comamonas testosteroni
81
-
2-Methylcyclohexanol
pH 7.5, 25°C
Comamonas testosteroni
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Comamonas testosteroni
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-cyclohexanediol + ferricyanide
-
705187
Comamonas testosteroni
2-hydroxycyclohexanone + ferrocyanide
-
-
-
?
1,3-cyclohexanediol + ferricyanide
-
705187
Comamonas testosteroni
3-hydroxycyclohexanone + ferrocyanide
-
-
-
?
1-butanol + ferricyanide
-
705187
Comamonas testosteroni
butanal + ferrocyanide
-
-
-
?
1-octanol + ferricyanide
-
705187
Comamonas testosteroni
octanal + ferrocyanide
-
-
-
?
1-pentanol + ferricyanide
-
705187
Comamonas testosteroni
pentanal + ferrocyanide
-
-
-
?
1-propanol + ferricyanide
-
705187
Comamonas testosteroni
propanal + ferrocyanide
-
-
-
?
2-butanol + ferricyanide
-
705187
Comamonas testosteroni
butanone + ferrocyanide
-
-
-
?
2-methylcyclohexanol + ferricyanide
-
705187
Comamonas testosteroni
2-methylcyclohexanone + ferrocyanide
-
-
-
?
2-octanol + ferricyanide
-
705187
Comamonas testosteroni
octan-2-one + ferrocyanide
-
-
-
?
2-pentanol + ferricyanide
-
705187
Comamonas testosteroni
pentan-2-one + ferrocyanide
-
-
-
?
2-propanol + ferricyanide
-
705187
Comamonas testosteroni
acetone + ferrocyanide
-
-
-
?
3-heptanol + ferricyanide
-
705187
Comamonas testosteroni
heptan-3-one + ferrocyanide
-
-
-
?
3-hexanol + ferricyanide
-
705187
Comamonas testosteroni
hexan-3-one + ferrocyanide
-
-
-
?
3-methylcyclohexanol + ferricyanide
-
705187
Comamonas testosteroni
3-methylcyclohexanone + ferrocyanide
-
-
-
?
3-nonanol + ferricyanide
-
705187
Comamonas testosteroni
nonan-3-one + ferrocyanide
-
-
-
?
3-octanol + ferricyanide
-
705187
Comamonas testosteroni
octan-3-one + ferrocyanide
-
-
-
?
3-pentanol + ferricyanide
-
705187
Comamonas testosteroni
pentan-3-one + ferrocyanide
-
-
-
?
4-decanol + ferricyanide
-
705187
Comamonas testosteroni
decan-4-one + ferrocyanide
-
-
-
?
4-heptanol + ferricyanide
-
705187
Comamonas testosteroni
heptan-4-one + ferrocyanide
-
-
-
?
cyclohexanol + ferricyanide
-
705187
Comamonas testosteroni
cyclohexanal + ferrocyanide
-
-
-
?
ethanal + ferrocyanide
-
705187
Comamonas testosteroni
ethanol + ferricyanide
-
-
-
r
ethanol + ferricyanide
-
705187
Comamonas testosteroni
ethanal + ferrocyanide
-
-
-
r
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
1-Octanol
pH 7.5, 25°C
Comamonas testosteroni
0.005
-
1-Pentanol
pH 7.5, 25°C
Comamonas testosteroni
0.006
-
1-butanol
pH 7.5, 25°C
Comamonas testosteroni
0.05
-
2-Octanol
pH 7.5, 25°C
Comamonas testosteroni
0.06
-
1-propanol
pH 7.5, 25°C
Comamonas testosteroni
0.11
-
3-Methylcyclohexanol
pH 7.5, 25°C
Comamonas testosteroni
0.4
-
4-decanol
pH 7.5, 25°C
Comamonas testosteroni
0.6
-
2-Pentanol
pH 7.5, 25°C
Comamonas testosteroni
0.7
-
4-heptanol
pH 7.5, 25°C
Comamonas testosteroni
0.7
-
Ethanal
pH 7.5, 25°C
Comamonas testosteroni
0.8
-
3-nonanol
pH 7.5, 25°C
Comamonas testosteroni
0.83
-
Cyclohexanol
pH 7.5, 25°C
Comamonas testosteroni
0.9
-
3-octanol
pH 7.5, 25°C
Comamonas testosteroni
2.2
-
ethanol
pH 7.5, 25°C
Comamonas testosteroni
2.9
-
3-Heptanol
pH 7.5, 25°C
Comamonas testosteroni
5.3
-
3-Hexanol
pH 7.5, 25°C
Comamonas testosteroni
8
-
2-butanol
pH 7.5, 25°C
Comamonas testosteroni
30
-
2-propanol
pH 7.5, 25°C
Comamonas testosteroni
30
-
3-Pentanol
pH 7.5, 25°C
Comamonas testosteroni
50
-
1,3-cyclohexandiol
pH 7.5, 25°C
Comamonas testosteroni
81
-
2-Methylcyclohexanol
pH 7.5, 25°C
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-cyclohexanediol + ferricyanide
-
705187
Comamonas testosteroni
2-hydroxycyclohexanone + ferrocyanide
-
-
-
?
1,3-cyclohexanediol + ferricyanide
-
705187
Comamonas testosteroni
3-hydroxycyclohexanone + ferrocyanide
-
-
-
?
1-butanol + ferricyanide
-
705187
Comamonas testosteroni
butanal + ferrocyanide
-
-
-
?
1-octanol + ferricyanide
-
705187
Comamonas testosteroni
octanal + ferrocyanide
-
-
-
?
1-pentanol + ferricyanide
-
705187
Comamonas testosteroni
pentanal + ferrocyanide
-
-
-
?
1-propanol + ferricyanide
-
705187
Comamonas testosteroni
propanal + ferrocyanide
-
-
-
?
2-butanol + ferricyanide
-
705187
Comamonas testosteroni
butanone + ferrocyanide
-
-
-
?
2-methylcyclohexanol + ferricyanide
-
705187
Comamonas testosteroni
2-methylcyclohexanone + ferrocyanide
-
-
-
?
2-octanol + ferricyanide
-
705187
Comamonas testosteroni
octan-2-one + ferrocyanide
-
-
-
?
2-pentanol + ferricyanide
-
705187
Comamonas testosteroni
pentan-2-one + ferrocyanide
-
-
-
?
2-propanol + ferricyanide
-
705187
Comamonas testosteroni
acetone + ferrocyanide
-
-
-
?
3-heptanol + ferricyanide
-
705187
Comamonas testosteroni
heptan-3-one + ferrocyanide
-
-
-
?
3-hexanol + ferricyanide
-
705187
Comamonas testosteroni
hexan-3-one + ferrocyanide
-
-
-
?
3-methylcyclohexanol + ferricyanide
-
705187
Comamonas testosteroni
3-methylcyclohexanone + ferrocyanide
-
-
-
?
3-nonanol + ferricyanide
-
705187
Comamonas testosteroni
nonan-3-one + ferrocyanide
-
-
-
?
3-octanol + ferricyanide
-
705187
Comamonas testosteroni
octan-3-one + ferrocyanide
-
-
-
?
3-pentanol + ferricyanide
-
705187
Comamonas testosteroni
pentan-3-one + ferrocyanide
-
-
-
?
4-decanol + ferricyanide
-
705187
Comamonas testosteroni
decan-4-one + ferrocyanide
-
-
-
?
4-heptanol + ferricyanide
-
705187
Comamonas testosteroni
heptan-4-one + ferrocyanide
-
-
-
?
cyclohexanol + ferricyanide
-
705187
Comamonas testosteroni
cyclohexanal + ferrocyanide
-
-
-
?
ethanal + ferrocyanide
-
705187
Comamonas testosteroni
ethanol + ferricyanide
-
-
-
r
ethanol + ferricyanide
-
705187
Comamonas testosteroni
ethanal + ferrocyanide
-
-
-
r
Other publictions for EC 1.1.9.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
654868
Jongejan
Deuterium isotope effect on en ...
Comamonas testosteroni
Biochim. Biophys. Acta
1647
297-302
2003
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
656080
Oubrie
Crystal structure of quinohemo ...
Comamonas testosteroni
J. Biol. Chem.
277
3727-3732
2002
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678768
Toyama
Azurin involved in alcohol oxi ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
65
1617-1626
2001
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701480
Oubrie
Crystallization of quinohemopr ...
Comamonas testosteroni
Acta Crystallogr. Sect. D
D57
1732-1734
2001
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701774
Somers
-
Enantioselective oxidation of ...
Comamonas testosteroni
Appl. Biochem. Biotechnol.
75
151-161
1999
-
1
-
-
-
-
-
50
-
-
-
-
-
1
-
-
-
-
-
-
-
-
35
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
50
-
-
-
-
-
-
-
-
-
-
-
-
35
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705187
Stigter
-
Enantioselective oxidation of ...
Comamonas testosteroni
J. Mol. Catal. B
2
291-297
1997
-
-
-
-
-
-
-
21
-
-
-
-
-
1
-
-
-
-
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389956
De Jong
Characterization of the intera ...
Comamonas testosteroni
Biochemistry
34
9451-9458
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389960
De Jong
Quinohemoprotein ethanol dehyd ...
Comamonas testosteroni
Eur. J. Biochem.
230
899-905
1995
-
-
-
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687362
Toyama
Three distinct quinoprotein al ...
Pseudomonas putida
J. Bacteriol.
177
2442-2450
1995
-
-
-
-
-
-
-
7
-
-
2
-
-
1
-
-
-
-
-
-
1
-
26
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
1
-
26
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
703526
Geerlof
Description of the kinetic mec ...
Comamonas testosteroni
Eur. J. Biochem.
226
537-546
1994
-
-
-
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
7
-
-
-
-
-
-
-
-
6
6
389953
Groen
Quinohaemoprotein alcohol dehy ...
Comamonas testosteroni
Biochem. J.
234
611-615
1986
-
-
-
-
-
-
-
12
-
1
2
-
-
1
-
-
-
-
-
-
-
2
15
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
12
-
1
2
-
-
-
-
-
-
-
-
2
15
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-