Enantioselective oxidation of secondary alcohols by quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.005

-

1-Pentanol

pH 7.5, 25°C

Comamonas testosteroni

0.005

-

1-Octanol

pH 7.5, 25°C

Comamonas testosteroni

0.006

-

1-butanol

pH 7.5, 25°C

Comamonas testosteroni

0.05

-

2-Octanol

pH 7.5, 25°C

Comamonas testosteroni

0.06

-

1-propanol

pH 7.5, 25°C

Comamonas testosteroni

0.11

-

3-Methylcyclohexanol

pH 7.5, 25°C

Comamonas testosteroni

0.4

-

4-decanol

pH 7.5, 25°C

Comamonas testosteroni

0.6

-

2-Pentanol

pH 7.5, 25°C

Comamonas testosteroni

0.7

-

Ethanal

pH 7.5, 25°C

Comamonas testosteroni

0.7

-

4-heptanol

pH 7.5, 25°C

Comamonas testosteroni

0.8

-

3-nonanol

pH 7.5, 25°C

Comamonas testosteroni

0.83

-

Cyclohexanol

pH 7.5, 25°C

Comamonas testosteroni

0.9

-

3-octanol

pH 7.5, 25°C

Comamonas testosteroni

2.2

-

ethanol

pH 7.5, 25°C

Comamonas testosteroni

2.9

-

3-Heptanol

pH 7.5, 25°C

Comamonas testosteroni

5.3

-

3-Hexanol

pH 7.5, 25°C

Comamonas testosteroni

8

-

2-butanol

pH 7.5, 25°C

Comamonas testosteroni

30

-

2-propanol

pH 7.5, 25°C

Comamonas testosteroni

30

-

3-Pentanol

pH 7.5, 25°C

Comamonas testosteroni

50

-

1,3-cyclohexandiol

pH 7.5, 25°C

Comamonas testosteroni

81

-

2-Methylcyclohexanol

pH 7.5, 25°C

Comamonas testosteroni

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

1,2-cyclohexanediol + ferricyanide

-

705187

Comamonas testosteroni

2-hydroxycyclohexanone + ferrocyanide

-

-

-

?

1,3-cyclohexanediol + ferricyanide

-

705187

Comamonas testosteroni

3-hydroxycyclohexanone + ferrocyanide

-

-

-

?

1-butanol + ferricyanide

-

705187

Comamonas testosteroni

butanal + ferrocyanide

-

-

-

?

1-octanol + ferricyanide

-

705187

Comamonas testosteroni

octanal + ferrocyanide

-

-

-

?

1-pentanol + ferricyanide

-

705187

Comamonas testosteroni

pentanal + ferrocyanide

-

-

-

?

1-propanol + ferricyanide

-

705187

Comamonas testosteroni

propanal + ferrocyanide

-

-

-

?

2-butanol + ferricyanide

-

705187

Comamonas testosteroni

butanone + ferrocyanide

-

-

-

?

2-methylcyclohexanol + ferricyanide

-

705187

Comamonas testosteroni

2-methylcyclohexanone + ferrocyanide

-

-

-

?

2-octanol + ferricyanide

-

705187

Comamonas testosteroni

octan-2-one + ferrocyanide

-

-

-

?

2-pentanol + ferricyanide

-

705187

Comamonas testosteroni

pentan-2-one + ferrocyanide

-

-

-

?

2-propanol + ferricyanide

-

705187

Comamonas testosteroni

acetone + ferrocyanide

-

-

-

?

3-heptanol + ferricyanide

-

705187

Comamonas testosteroni

heptan-3-one + ferrocyanide

-

-

-

?

3-hexanol + ferricyanide

-

705187

Comamonas testosteroni

hexan-3-one + ferrocyanide

-

-

-

?

3-methylcyclohexanol + ferricyanide

-

705187

Comamonas testosteroni

3-methylcyclohexanone + ferrocyanide

-

-

-

?

3-nonanol + ferricyanide

-

705187

Comamonas testosteroni

nonan-3-one + ferrocyanide

-

-

-

?

3-octanol + ferricyanide

-

705187

Comamonas testosteroni

octan-3-one + ferrocyanide

-

-

-

?

3-pentanol + ferricyanide

-

705187

Comamonas testosteroni

pentan-3-one + ferrocyanide

-

-

-

?

4-decanol + ferricyanide

-

705187

Comamonas testosteroni

decan-4-one + ferrocyanide

-

-

-

?

4-heptanol + ferricyanide

-

705187

Comamonas testosteroni

heptan-4-one + ferrocyanide

-

-

-

?

cyclohexanol + ferricyanide

-

705187

Comamonas testosteroni

cyclohexanal + ferrocyanide

-

-

-

?

ethanal + ferrocyanide

-

705187

Comamonas testosteroni

ethanol + ferricyanide

-

-

-

r

ethanol + ferricyanide

-

705187

Comamonas testosteroni

ethanal + ferrocyanide

-

-

-

r

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.005

-

1-Pentanol

pH 7.5, 25°C

Comamonas testosteroni

0.005

-

1-Octanol

pH 7.5, 25°C

Comamonas testosteroni

0.006

-

1-butanol

pH 7.5, 25°C

Comamonas testosteroni

0.05

-

2-Octanol

pH 7.5, 25°C

Comamonas testosteroni

0.06

-

1-propanol

pH 7.5, 25°C

Comamonas testosteroni

0.11

-

3-Methylcyclohexanol

pH 7.5, 25°C

Comamonas testosteroni

0.4

-

4-decanol

pH 7.5, 25°C

Comamonas testosteroni

0.6

-

2-Pentanol

pH 7.5, 25°C

Comamonas testosteroni

0.7

-

Ethanal

pH 7.5, 25°C

Comamonas testosteroni

0.7

-

4-heptanol

pH 7.5, 25°C

Comamonas testosteroni

0.8

-

3-nonanol

pH 7.5, 25°C

Comamonas testosteroni

0.83

-

Cyclohexanol

pH 7.5, 25°C

Comamonas testosteroni

0.9

-

3-octanol

pH 7.5, 25°C

Comamonas testosteroni

2.2

-

ethanol

pH 7.5, 25°C

Comamonas testosteroni

2.9

-

3-Heptanol

pH 7.5, 25°C

Comamonas testosteroni

5.3

-

3-Hexanol

pH 7.5, 25°C

Comamonas testosteroni

8

-

2-butanol

pH 7.5, 25°C

Comamonas testosteroni

30

-

2-propanol

pH 7.5, 25°C

Comamonas testosteroni

30

-

3-Pentanol

pH 7.5, 25°C

Comamonas testosteroni

50

-

1,3-cyclohexandiol

pH 7.5, 25°C

Comamonas testosteroni

81

-

2-Methylcyclohexanol

pH 7.5, 25°C

Comamonas testosteroni

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

1,2-cyclohexanediol + ferricyanide

-

705187

Comamonas testosteroni

2-hydroxycyclohexanone + ferrocyanide

-

-

-

?

1,3-cyclohexanediol + ferricyanide

-

705187

Comamonas testosteroni

3-hydroxycyclohexanone + ferrocyanide

-

-

-

?

1-butanol + ferricyanide

-

705187

Comamonas testosteroni

butanal + ferrocyanide

-

-

-

?

1-octanol + ferricyanide

-

705187

Comamonas testosteroni

octanal + ferrocyanide

-

-

-

?

1-pentanol + ferricyanide

-

705187

Comamonas testosteroni

pentanal + ferrocyanide

-

-

-

?

1-propanol + ferricyanide

-

705187

Comamonas testosteroni

propanal + ferrocyanide

-

-

-

?

2-butanol + ferricyanide

-

705187

Comamonas testosteroni

butanone + ferrocyanide

-

-

-

?

2-methylcyclohexanol + ferricyanide

-

705187

Comamonas testosteroni

2-methylcyclohexanone + ferrocyanide

-

-

-

?

2-octanol + ferricyanide

-

705187

Comamonas testosteroni

octan-2-one + ferrocyanide

-

-

-

?

2-pentanol + ferricyanide

-

705187

Comamonas testosteroni

pentan-2-one + ferrocyanide

-

-

-

?

2-propanol + ferricyanide

-

705187

Comamonas testosteroni

acetone + ferrocyanide

-

-

-

?

3-heptanol + ferricyanide

-

705187

Comamonas testosteroni

heptan-3-one + ferrocyanide

-

-

-

?

3-hexanol + ferricyanide

-

705187

Comamonas testosteroni

hexan-3-one + ferrocyanide

-

-

-

?

3-methylcyclohexanol + ferricyanide

-

705187

Comamonas testosteroni

3-methylcyclohexanone + ferrocyanide

-

-

-

?

3-nonanol + ferricyanide

-

705187

Comamonas testosteroni

nonan-3-one + ferrocyanide

-

-

-

?

3-octanol + ferricyanide

-

705187

Comamonas testosteroni

octan-3-one + ferrocyanide

-

-

-

?

3-pentanol + ferricyanide

-

705187

Comamonas testosteroni

pentan-3-one + ferrocyanide

-

-

-

?

4-decanol + ferricyanide

-

705187

Comamonas testosteroni

decan-4-one + ferrocyanide

-

-

-

?

4-heptanol + ferricyanide

-

705187

Comamonas testosteroni

heptan-4-one + ferrocyanide

-

-

-

?

cyclohexanol + ferricyanide

-

705187

Comamonas testosteroni

cyclohexanal + ferrocyanide

-

-

-

?

ethanal + ferrocyanide

-

705187

Comamonas testosteroni

ethanol + ferricyanide

-

-

-

r

ethanol + ferricyanide

-

705187

Comamonas testosteroni

ethanal + ferrocyanide

-

-

-

r

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

654868

Jongejan

Deuterium isotope effect on en ...

Comamonas testosteroni

Biochim. Biophys. Acta

1647

297-302

2003

-

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

656080

Oubrie

Crystal structure of quinohemo ...

Comamonas testosteroni

J. Biol. Chem.

277

3727-3732

2002

-

-

-

1

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

678768

Toyama

Azurin involved in alcohol oxi ...

Pseudomonas putida

Biosci. Biotechnol. Biochem.

65

1617-1626

2001

-

-

1

-

-

-

-

-

-

-

2

-

-

1

-

-

-

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

701480

Oubrie

Crystallization of quinohemopr ...

Comamonas testosteroni

Acta Crystallogr. Sect. D

D57

1732-1734

2001

-

-

-

1

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

701774

Somers

-

Enantioselective oxidation of ...

Comamonas testosteroni

Appl. Biochem. Biotechnol.

75

151-161

1999

-

1

-

-

-

-

-

59

-

-

-

-

-

1

-

-

-

-

-

-

-

-

36

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

59

-

-

-

-

-

-

-

-

-

-

-

-

36

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

705187

Stigter

-

Enantioselective oxidation of ...

Comamonas testosteroni

J. Mol. Catal. B

2

291-297

1997

-

-

-

-

-

-

-

21

-

-

-

-

-

1

-

-

-

-

-

-

-

-

22

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

21

-

-

-

-

-

-

-

-

-

-

-

-

22

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

389956

De Jong

Characterization of the intera ...

Comamonas testosteroni

Biochemistry

34

9451-9458

1995

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

389960

De Jong

Quinohemoprotein ethanol dehyd ...

Comamonas testosteroni

Eur. J. Biochem.

230

899-905

1995

-

-

-

-

-

-

-

-

-

1

2

-

-

1

-

-

1

-

-

-

1

-

1

1

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

1

2

-

-

-

-

1

-

-

1

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

687362

Toyama

Three distinct quinoprotein al ...

Pseudomonas putida

J. Bacteriol.

177

2442-2450

1995

-

-

-

-

-

-

-

7

-

-

2

-

-

1

-

-

-

-

-

-

1

-

26

1

2

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

7

-

-

2

-

-

-

-

-

-

-

1

-

26

1

-

-

-

-

-

-

-

-

-

1

1

-

-

-

703526

Geerlof

Description of the kinetic mec ...

Comamonas testosteroni

Eur. J. Biochem.

226

537-546

1994

-

-

-

-

-

-

-

6

-

-

-

-

-

1

-

-

-

-

-

-

-

-

7

-

-

-

-

-

7

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

6

-

-

-

-

-

-

-

-

-

-

-

-

7

-

-

-

-

7

-

-

-

-

-

-

-

-

6

6

389953

Groen

Quinohaemoprotein alcohol dehy ...

Comamonas testosteroni

Biochem. J.

234

611-615

1986

-

-

-

-

-

-

-

12

-

1

2

-

-

1

-

-

-

-

-

-

-

2

15

1

1

-

-

-

-

1

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

12

-

1

2

-

-

-

-

-

-

-

-

2

15

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-