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Literature summary for 1.1.98.2 extracted from

  • Bashiri, G.; Perkowski, E.; Turner, A.; Feltcher, M.; Braunstein, M.; Baker, E.
    Tat-dependent translocation of an F420-binding protein of Mycobacterium tuberculosis (2012), PLoS ONE, 7, e45003.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Rv0132c, sequence comparisons and homology modeling of Rv0132c, expression of Rv0132c-D38 construct lacking the predicted N-terminal signal sequence in Mycobacterium smegmatis by nickel affinity chromatography and gel filtration, co-expression with coenzyme F420 Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35200
-
2 * 35200, SDS-PAGE, recombinant Rv0132c-D38 construct lacking the predicted N-terminal signal sequence Mycobacterium tuberculosis
75000
-
gel filtration, recombinant Rv0132c-D38 construct lacking the predicted N-terminal signal sequence Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + oxidized coenzyme F420 Mycobacterium tuberculosis
-
6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420
-
?
D-glucose 6-phosphate + oxidized coenzyme F420 Mycobacterium tuberculosis H37Rv
-
6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + oxidized coenzyme F420
-
Mycobacterium tuberculosis 6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420
-
?
D-glucose 6-phosphate + oxidized coenzyme F420
-
Mycobacterium tuberculosis H37Rv 6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 35200, SDS-PAGE, recombinant Rv0132c-D38 construct lacking the predicted N-terminal signal sequence Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
F420-dependent glucose-6-phosphate dehydrogenase
-
Mycobacterium tuberculosis
FGD
-
Mycobacterium tuberculosis
FGD1
-
Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
coenzyme F420 a 5-deazaflavin derivative Mycobacterium tuberculosis

General Information

General Information Comment Organism
additional information Rv0132c is mis-annotated as an F420-dependent glucose-6-phosphate dehydrogenase, the protein encoded by gene Rv0132c does not show F420-dependent glucose-6-phosphate dehydrogenase activity. Three phosphate binding residues in FGD1, Lys198, Lys259, and Arg283, are not present in Rv0132c. Helix alpga9 in FGD1 is replaced by a smaller loop in Rv0132c, due to a deletion of four residues in this region. This expands the active site cavity for Rv0132c since this helix is part of the structure that caps the barrel to create the active site cavity. whereas Rv0132c, like FGD1, has the ability to bind F420, the two enzymes probably act on different substrates and catalyze different reactions. Structural comparison of Rv0132c with FGD1, overview. The Rv0132c-F420 complex is a substrate for the Tat pathway, which mediates translocation of the complex across the cytoplasmic membrane, where Rv0132c is anchored to the cell envelope Mycobacterium tuberculosis