Cloned (Comment) | Organism |
---|---|
gene Rv0132c, sequence comparisons and homology modeling of Rv0132c, expression of Rv0132c-D38 construct lacking the predicted N-terminal signal sequence in Mycobacterium smegmatis by nickel affinity chromatography and gel filtration, co-expression with coenzyme F420 | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35200 | - |
2 * 35200, SDS-PAGE, recombinant Rv0132c-D38 construct lacking the predicted N-terminal signal sequence | Mycobacterium tuberculosis |
75000 | - |
gel filtration, recombinant Rv0132c-D38 construct lacking the predicted N-terminal signal sequence | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + oxidized coenzyme F420 | Mycobacterium tuberculosis | - |
6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420 | - |
? | |
D-glucose 6-phosphate + oxidized coenzyme F420 | Mycobacterium tuberculosis H37Rv | - |
6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + oxidized coenzyme F420 | - |
Mycobacterium tuberculosis | 6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420 | - |
? | |
D-glucose 6-phosphate + oxidized coenzyme F420 | - |
Mycobacterium tuberculosis H37Rv | 6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35200, SDS-PAGE, recombinant Rv0132c-D38 construct lacking the predicted N-terminal signal sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
F420-dependent glucose-6-phosphate dehydrogenase | - |
Mycobacterium tuberculosis |
FGD | - |
Mycobacterium tuberculosis |
FGD1 | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F420 | a 5-deazaflavin derivative | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
additional information | Rv0132c is mis-annotated as an F420-dependent glucose-6-phosphate dehydrogenase, the protein encoded by gene Rv0132c does not show F420-dependent glucose-6-phosphate dehydrogenase activity. Three phosphate binding residues in FGD1, Lys198, Lys259, and Arg283, are not present in Rv0132c. Helix alpga9 in FGD1 is replaced by a smaller loop in Rv0132c, due to a deletion of four residues in this region. This expands the active site cavity for Rv0132c since this helix is part of the structure that caps the barrel to create the active site cavity. whereas Rv0132c, like FGD1, has the ability to bind F420, the two enzymes probably act on different substrates and catalyze different reactions. Structural comparison of Rv0132c with FGD1, overview. The Rv0132c-F420 complex is a substrate for the Tat pathway, which mediates translocation of the complex across the cytoplasmic membrane, where Rv0132c is anchored to the cell envelope | Mycobacterium tuberculosis |