Any feedback?
Literature summary for 1.1.98.6 extracted from
- Allosteric regulation of the class III anaerobic ribonucleotide reductase from bacteriophage T4 (2000), J. Biol. Chem., 275, 19443-19448.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | positive effector for CTP reduction | Tequatrovirus T4 |  |
| dATP | positive effector for CTP reduction | Tequatrovirus T4 | |
| dGTP | positive effector for ATP reduction | Tequatrovirus T4 | |
| dTTP | positive effector for GTP reduction | Tequatrovirus T4 | |
| additional information | enzyme has a single type of allosteric site to which dGTP, dTTP, dATP, and ATP bind competitively | Tequatrovirus T4 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N311A | about 20% of wild-type activtiy | Tequatrovirus T4 |
| N311C | less than 1% of wild-type activtiy | Tequatrovirus T4 |
| N78A | less than 10% of wild-type activtiy | Tequatrovirus T4 |
| N78A/N311A | less than 1% of wild-type activtiy | Tequatrovirus T4 |
| N78C | 1% of wild-type activtiy | Tequatrovirus T4 |
| N78D | 1% of wild-type activtiy | Tequatrovirus T4 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | P07071 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.6 | - |
mutant N78A/N311A, pH 8.0, temperature not specified in the publication | Tequatrovirus T4 |
| 5.6 | - |
mutant N311C, pH 8.0, temperature not specified in the publication | Tequatrovirus T4 |
| 10.5 | - |
mutant N78D, pH 8.0, temperature not specified in the publication | Tequatrovirus T4 |
| 11.8 | - |
mutant N78C, pH 8.0, temperature not specified in the publication | Tequatrovirus T4 |
| 87.6 | - |
mutant N78A, pH 8.0, temperature not specified in the publication | Tequatrovirus T4 |
| 207 | - |
mutant N311A, pH 8.0, temperature not specified in the publication | Tequatrovirus T4 |
| 370 | - |
substrate CTP, pH not specified in the publication, temperature not specified in the publication | Tequatrovirus T4 |
| 575 | - |
substrate CTP, presence of ATP, pH not specified in the publication, temperature not specified in the publication | Tequatrovirus T4 |
| 730 | - |
substrate CTP, presence of dATP, pH not specified in the publication, temperature not specified in the publication | Tequatrovirus T4 |
| 1155 | - |
wild-type, pH 8.0, temperature not specified in the publication | Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + formate | - |
Tequatrovirus T4 | dATP + CO2 + H2O | - |
? | |
| CTP + formate | - |
Tequatrovirus T4 | dCTP + CO2 + H2O | - |
? | |
| GTP + formate | - |
Tequatrovirus T4 | dGTP + CO2 + H2O | - |
? | |
| additional information | no significant UTP reduction under the conditions used | Tequatrovirus T4 | ? | - |
? | |
| TTP + formate | - |
Tequatrovirus T4 | dTTP + CO2 + H2O | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
