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Literature summary for 1.1.99.18 extracted from

  • Coudray, M.R.; Canevascini, G.; Meier, H.
    Characterization of a cellobiose dehydrogenase in the cellulolytic fungus Sporotrichum (Chrysosporium) thermophile (1982), Biochem. J., 203, 277-284.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0069
-
cellobiose
-
Thermothelomyces heterothallicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular bound to mycelium from which it is released into the extracellular medium Thermothelomyces heterothallicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates activity up to 2fold Thermothelomyces heterothallicus

Organism

Organism UniProt Comment Textmining
Thermothelomyces heterothallicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Thermothelomyces heterothallicus
-
culture medium bound to mycelium from which it is released into the extracellular medium Thermothelomyces heterothallicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-beta-glucosylmannose + acceptor 47% the rate of cellobiose oxidation Thermothelomyces heterothallicus ?
-
?
cellobiose + acceptor electron acceptors: phenol blue, Fe3+, methylene blue, 3,5-di-tert-butyl-1,2-benzoquinone, p-benzoquinone, potassium ferricyanide, cytochrome c Thermothelomyces heterothallicus cellobiono-1,5-lactone + reduced acceptor
-
?
cellotetraose + acceptor
-
Thermothelomyces heterothallicus cellotetrono-1,5-lactone + reduced acceptor
-
?
cellotriose + acceptor
-
Thermothelomyces heterothallicus cellotriono-1,5-lactone + reduced acceptor
-
?
lactose + acceptor
-
Thermothelomyces heterothallicus 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?
xylobiose + acceptor 38% the rate of cellobiose oxidation Thermothelomyces heterothallicus 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced acceptor
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4.5 acceptor: methylene blue Thermothelomyces heterothallicus
5 6 acceptor: 2,6-dichlorophenolindophenol Thermothelomyces heterothallicus
7
-
acceptor: 3,5-di-tert-butyl-1,2-benzoquinone Thermothelomyces heterothallicus
8 9 acceptor: phenazine methosulfate Thermothelomyces heterothallicus