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Literature summary for 1.1.99.18 extracted from

  • Ludwig, R.; Salamon, A.; Varga, J.; Zamocky, M.; Peterbauer, C.K.; Kulbe, K.D.; Haltrich, D.
    Characterization of cellobiose dehydrogenase from the white rot fungi Trametes pubescens and Trametes villosa (2004), Appl. Microbiol. Biotechnol., 64, 213-222.
    View publication on PubMed
Search for more literature for 1.1.99.18

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00069
-
 cytochrome c pH 4.5, 30°C, reaction with lactose Trametes pubescens
0.00099
-
 ferricenium ion pH 4.5, 30°C, reaction with lactose Trametes pubescens
0.0012
-
 ferricenium ion pH 4.5, 30°C, reaction with lactose Trametes villosa
0.0018
-
 cytochrome c pH 4.5, 30°C, reaction with lactose Trametes villosa
0.0049
-
 2,6-dichlorophenol indophenol pH 4.5, 30°C, reaction with lactose Trametes pubescens
0.0055
-
 K3Fe(CN)6 pH 4.5, 30°C, reaction with lactose Trametes villosa
0.0086
-
 K3Fe(CN)6 pH 4.5, 30°C, reaction with lactose Trametes pubescens
0.0099
-
 2,6-dichlorophenol indophenol pH 4.5, 30°C, reaction with lactose Trametes villosa
0.011
-
 1,4-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes villosa
0.018
-
 3,5-di-tert-butyl-1,2-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes pubescens
0.019
-
 1,4-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes pubescens
0.041
-
 3,5-di-tert-butyl-1,2-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes villosa
0.21
-
 cellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
0.21
-
 cellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
0.42
-
 cellotetraose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
0.46
-
 cellopentaose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
0.47
-
 cellotetraose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
0.51
-
 cellopentaose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.8
-
 thiocellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
2.4
-
 lactose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
2.5
-
 thiocellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
3.7
-
 lactose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
7.3
-
 xylobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
7.7
-
 xylobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
350
-
 maltose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
390
-
 maltose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
890
-
 D-glucose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1300
-
 D-glucose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trametes villosa
-
-
extracellular
-
 Trametes pubescens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
 gel filtration Trametes villosa
90000
-
 gel filtration Trametes pubescens
98000
-
 1 * 98000, SDS-PAGE Trametes villosa
100000
-
 1 * 100000, SDS-PAGE Trametes pubescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trametes versicolor proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes hirsuta proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes ochracea proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes villosa proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes pubescens proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes maxima proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes gibbosa proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes cotonea proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes incerta proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes meyenii proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes suaveolens proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes villosa CBS 334.49 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes suaveolens MB 51 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes ochracea MB 49 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes versicolor MB 52 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes hirsuta MB 50 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes maxima CBS 250.69 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes gibbosa MB 187 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes cotonea CBS 352.80 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
additional information Trametes incerta CBS 455.76 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trametes cotonea
-
 CBS 352.80
-
Trametes cotonea CBS 352.80
-
 CBS 352.80
-
Trametes gibbosa
-
 MB 187
-
Trametes gibbosa MB 187
-
 MB 187
-
Trametes hirsuta
-
 MB 50
-
Trametes hirsuta MB 50
-
 MB 50
-
Trametes incerta
-
 CBS 455.76
-
Trametes incerta CBS 455.76
-
 CBS 455.76
-
Trametes maxima
-
 CBS 250.69
-
Trametes maxima CBS 250.69
-
 CBS 250.69
-
Trametes meyenii
-
 CBS 453.76, weak activity
-
Trametes ochracea
-
 MB 49
-
Trametes ochracea MB 49
-
 MB 49
-
Trametes pubescens
-
-
-
Trametes suaveolens
-
 MB 51
-
Trametes suaveolens MB 51
-
 MB 51
-
Trametes versicolor
-
 MB 52
-
Trametes versicolor MB 52
-
 MB 52
-
Trametes villosa
-
 CBS 334.49
-
Trametes villosa CBS 334.49
-
 CBS 334.49
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trametes villosa
-
 Trametes pubescens

Source Tissue

Source Tissue Comment Organism Textmining
culture medium cellulose-containing Trametes villosa
-
culture medium cellulose-containing Trametes pubescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62
-
 assay detects both intact and truncated enzyme Trametes pubescens
69
-
 assay detects both intact and truncated enzyme Trametes villosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenol indophenol
-
 Trametes villosa cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellobiose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellobiose + 2,6-dichlorophenol indophenol
-
 Trametes villosa CBS 334.49 cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellopentaose + 2,6-dichlorophenol indophenol
-
 Trametes villosa cellopentaono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellopentaose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens cellopentaono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellopentaose + 2,6-dichlorophenol indophenol
-
 Trametes villosa CBS 334.49 cellopentaono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellotetraose + 2,6-dichlorophenol indophenol
-
 Trametes villosa cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellotetraose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellotetraose + 2,6-dichlorophenol indophenol
-
 Trametes villosa CBS 334.49 cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
D-glucose + 2,6-dichlorophenol indophenol
-
 Trametes villosa D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
D-glucose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
D-glucose + 2,6-dichlorophenol indophenol
-
 Trametes villosa CBS 334.49 D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 1,4-benzoquinone
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + 1,4-benzoquinol
-
 ?
lactose + 1,4-benzoquinone
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + 1,4-benzoquinol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes versicolor 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes hirsuta 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes ochracea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes maxima 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes gibbosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes cotonea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes incerta 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes meyenii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes suaveolens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes suaveolens MB 51 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes ochracea MB 49 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes versicolor MB 52 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes hirsuta MB 50 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes maxima CBS 250.69 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes gibbosa MB 187 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes cotonea CBS 352.80 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Trametes incerta CBS 455.76 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 3,5-di-tert-butyl-1,2-benzoquinone
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
-
 ?
lactose + 3,5-di-tert-butyl-1,2-benzoquinone
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
-
 ?
lactose + cytochrome c
-
 Trametes versicolor 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes hirsuta 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes ochracea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes maxima 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes gibbosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes cotonea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes incerta 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes meyenii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes suaveolens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes suaveolens MB 51 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes ochracea MB 49 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes versicolor MB 52 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes hirsuta MB 50 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes maxima CBS 250.69 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes gibbosa MB 187 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes cotonea CBS 352.80 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Trametes incerta CBS 455.76 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + Fe(CN)63-
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + Fe(CN)64-
-
 ?
lactose + Fe(CN)63-
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + Fe(CN)64-
-
 ?
lactose + ferricenium ion
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
-
 ?
lactose + ferricenium ion
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
-
 ?
lactose + K3Fe(CN)6
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced K3Fe(CN)6
-
 r
lactose + K3Fe(CN)6
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced K3Fe(CN)6
-
 r
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes versicolor ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes hirsuta ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes ochracea ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes villosa ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes pubescens ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes maxima ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes gibbosa ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes cotonea ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes incerta ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes meyenii ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes suaveolens ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes villosa CBS 334.49 ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes suaveolens MB 51 ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes ochracea MB 49 ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes versicolor MB 52 ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes hirsuta MB 50 ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes maxima CBS 250.69 ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes gibbosa MB 187 ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes cotonea CBS 352.80 ?
-
 ?
additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes incerta CBS 455.76 ?
-
 ?
thiocellobiose + 2,6-dichlorophenol indophenol
-
 Trametes villosa thiocellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
thiocellobiose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens thiocellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
xylobiose + 2,6-dichlorophenol indophenol
-
 Trametes villosa 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
xylobiose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Trametes pubescens
monomer 1 * 98000, SDS-PAGE Trametes villosa

Synonyms

Synonyms Comment Organism
Cdh
-
 Trametes versicolor
Cdh
-
 Trametes hirsuta
Cdh
-
 Trametes ochracea
Cdh
-
 Trametes maxima
Cdh
-
 Trametes gibbosa
Cdh
-
 Trametes cotonea
Cdh
-
 Trametes incerta
Cdh
-
 Trametes meyenii
Cdh
-
 Trametes suaveolens
TpCDH
-
 Trametes pubescens
TvCDH
-
 Trametes villosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
24
-
 stable Trametes villosa
24
-
 6 h, 23% loss of activity Trametes pubescens
30
-
 half-life: 160 min Trametes villosa
30
-
 half-life: 34 min Trametes pubescens
45
-
 half-life: 17 min Trametes pubescens
45
-
 half-life: 97 min Trametes villosa
60
-
 half-life: 2.5 min Trametes villosa
60
-
 half-life: less than 1 min Trametes pubescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.31
-
 D-glucose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.92
-
 maltose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.92
-
 glucose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
2.08
-
 maltose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
13.9
-
 cellopentaose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
14.2
-
 cellotetraose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
14.7
-
 cellopentaose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
15
-
 cellotetraose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
16.9
-
 3,5-di-tert-butyl-1,2-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes villosa
17.2
-
 xylobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
18.3
-
 xylobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
19.4
-
 thiocellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
20.8
-
 1,4-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes villosa
21.4
-
 thiocellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
21.9
-
 cellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
23.6
-
 cellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
24.2
-
 3,5-di-tert-butyl-1,2-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes pubescens
25.6
-
 2,6-dichlorophenol indophenol pH 4.5, 30°C, reaction with lactose Trametes pubescens
25.8
-
 lactose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
26.9
-
 lactose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
27.5
-
 1,4-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes pubescens
27.8
-
 K3Fe(CN)6 pH 4.5, 30°C, reaction with lactose Trametes pubescens
29.4
-
 cytochrome c pH 4.5, 30°C, reaction with lactose Trametes pubescens
32.5
-
 cytochrome c pH 4.5, 30°C, reaction with lactose Trametes villosa
34.7
-
 ferricenium ion pH 4.5, 30°C, reaction with lactose Trametes pubescens
35.6
-
 ferricenium ion pH 4.5, 30°C, reaction with lactose Trametes villosa
36.9
-
 K3Fe(CN)6 pH 4.5, 30°C, reaction with lactose Trametes villosa
38.1
-
 2,6-dichlorophenol indophenol pH 4.5, 30°C, reaction with lactose Trametes villosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 lactose + cytochrome c, in acetate buffer Trametes pubescens
3.5 4.5 lactose + cytochrome c, in acetate buffer Trametes villosa
4.5
-
 lactose + 2,6-dichlorophenol indophenol or cytochrome c, in citrate buffer Trametes pubescens
4.5
-
 lactose + cytochrome c, in citrate buffer Trametes villosa
5
-
 lactose + 2,6-dichlorophenol indophenol, in citrate buffer Trametes villosa

pH Range

pH Minimum pH Maximum Comment Organism
3 5 pH 3.0: about 60% of maximal activity, pH 5.0: about 70% of maximal activity, lactose + cytochrome c, in acetate buffer Trametes villosa
3 5 pH 3.0: about 70% of maximal activity, pH 5.0: about 40% of maximal activity, lactose + cytochrome c, in acetate buffer Trametes pubescens
3.5 5.5 pH 3.5: about 50% of maximal activity, pH 5.5: about 20% of maximal activity, lactose + 2,6-dichlorophenol indophenol, in citrate buffer Trametes pubescens
3.5 5.5 pH 3.5: about 50% of maximal activity, pH 5.5: about 20% of maximal activity, lactose + cytochrome c, in citrate buffer Trametes villosa
4 6 pH 4.0: about 70% of maximal activity, pH 6.0: about 40% of maximal activity, lactose + 2,6-dichlorophenol indophenol, in citrate buffer Trametes pubescens
4 6 pH 4.0: about 75% of maximal activity, pH 6.0: about 65% of maximal activity, lactose + 2,6-dichlorophenol indophenol, in citrate buffer Trametes villosa

Cofactor

Cofactor Comment Organism Structure
flavin haemoflavoprotein Trametes versicolor
flavin haemoflavoprotein Trametes hirsuta
flavin haemoflavoprotein Trametes ochracea
flavin haemoflavoprotein Trametes villosa
flavin haemoflavoprotein Trametes pubescens
flavin haemoflavoprotein Trametes maxima
flavin haemoflavoprotein Trametes gibbosa
flavin haemoflavoprotein Trametes cotonea
flavin haemoflavoprotein Trametes incerta
flavin haemoflavoprotein Trametes meyenii
flavin haemoflavoprotein Trametes suaveolens
heme haemoflavoprotein Trametes versicolor
heme haemoflavoprotein Trametes hirsuta
heme haemoflavoprotein Trametes ochracea
heme haemoflavoprotein Trametes villosa
heme haemoflavoprotein Trametes pubescens
heme haemoflavoprotein Trametes maxima
heme haemoflavoprotein Trametes gibbosa
heme haemoflavoprotein Trametes cotonea
heme haemoflavoprotein Trametes incerta
heme haemoflavoprotein Trametes meyenii
heme haemoflavoprotein Trametes suaveolens

pI Value

Organism Comment pI Value Maximum pI Value
Trametes villosa isoelectric focusing
-
 4.2
Trametes pubescens isoelectric focusing
-
 4.2