Cloned (Comment) | Organism |
---|---|
- |
Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
H689A | more than 1000fold lower turnover value, Km-value for cellobiose and lactose is similar to that of the wild-type enzyme | Phanerodontia chrysosporium |
H689E | more than 1000fold lower turnover value, Km-value for cellobiose and lactose is similar to that of the wild-type enzyme | Phanerodontia chrysosporium |
H689N | more than 1000fold lower turnover value, Km-value for cellobiose and lactose is similar to that of the wild-type enzyme | Phanerodontia chrysosporium |
H689Q | more than 1000fold lower turnover value, Km-value for cellobiose and lactose is similar to that of the wild-type enzyme | Phanerodontia chrysosporium |
H689V | more than 1000fold lower turnover value, Km-value for cellobiose and lactose is similar to that of the wild-type enzyme | Phanerodontia chrysosporium |
N732A | the turnover-number for cellobiose is 38.8fold lower than the turnover-number of the wild-type enzyme, the Km-value for cellobiose is 1.1fold higher than the KM-value of the wild-type enzyme, the turnover-number for lactose is 20.4fold lower than the turnover-number of the wild-type enzyme, the Km-value for lactose is 4.4fold higher than the KM-value of the wild-type enzyme | Phanerodontia chrysosporium |
N732D | the turnover-number for cellobiose is 3875fold lower than the turnover-number of the wild-type enzyme, the Km-value for cellobiose is 10.6fold higher than the KM-value of the wild-type enzyme, the turnover-number for lactose is 2860fold lower than the turnover-number of the wild-type enzyme, the Km-value for lactose is 42.2fold higher than the KM-value of the wild-type enzyme. The pH optimum is shifted from pH 3-5 for the wild-type enzyme in the reaction with cellobiose and 2,6-dichlorophenol-indophenol to pH 6.5-7.0. The pH optimum is shifted from pH 3 for the wild-type enzyme in the reaction with cellobiose and cytochrome c to pH 6 | Phanerodontia chrysosporium |
N732E | the turnover-number for cellobiose is 73.8fold lower than the turnover-number of the wild-type enzyme, the Km-value for cellobiose is 14.4fold higher than the KM-value of the wild-type enzyme, the turnover-number for lactose is 47.7fold lower than the turnover-number of the wild-type enzyme, the Km-value for lactose is 61.5fold higher than the KM-value of the wild-type enzyme. The pH optimum is shifted from pH 3-5 for the wild-type enzyme in the reaction with cellobiose and 2,6-dichlorophenol-indophenol to pH 6.5-7.0.The pH optimum is shifted from pH 3 for the wild-type enzyme in the reaction with cellobiose and cytochrome c to pH 5 | Phanerodontia chrysosporium |
N732H | the turnover-number for cellobiose is 5.7fold lower than the turnover-number of the wild-type enzyme, the Km-value for cellobiose is 2.4fold higher than the KM-value of the wild-type enzyme, the turnover-number for lactose is 8.4fold lower than the turnover-number of the wild-type enzyme, the Km-value for lactose is 8.5fold higher than the KM-value of the wild-type enzyme | Phanerodontia chrysosporium |
N732Q | the turnover-number for cellobiose is 15.5fold lower than the turnover-number of the wild-type enzyme, the Km-value for cellobiose is 2.6fold higher than the KM-value of the wild-type enzyme, the turnover-number for lactose is 11.9fold lower than the turnover-number of the wild-type enzyme, the Km-value for lactose is 14.8fold higher than the KM-value of the wild-type enzyme | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
cellobiose | pH 4.5, 23°C, recombinant wild-type enzyme | Phanerodontia chrysosporium | |
0.017 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme N732A | Phanerodontia chrysosporium | |
0.019 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme H689N | Phanerodontia chrysosporium | |
0.021 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme H689Q or mutant enzyme H689A | Phanerodontia chrysosporium | |
0.022 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme H689E | Phanerodontia chrysosporium | |
0.031 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme H689V | Phanerodontia chrysosporium | |
0.04 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme N732H | Phanerodontia chrysosporium | |
0.041 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme N732Q | Phanerodontia chrysosporium | |
0.17 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme N732D | Phanerodontia chrysosporium | |
0.23 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme N732E | Phanerodontia chrysosporium | |
0.27 | - |
lactose | pH 4.5, 23°C, recombinant wild-type enzyme | Phanerodontia chrysosporium | |
0.28 | - |
lactose | pH 4.5, 23°C, mutant enzyme H689A | Phanerodontia chrysosporium | |
0.29 | - |
lactose | pH 4.5, 23°C, mutant enzyme H689Q | Phanerodontia chrysosporium | |
1.2 | - |
lactose | pH 4.5, 23°C, mutant enzyme N732A | Phanerodontia chrysosporium | |
2.3 | - |
lactose | pH 4.5, 23°C, mutant enzyme N732H | Phanerodontia chrysosporium | |
4 | - |
lactose | pH 4.5, 23°C, mutant enzyme N732Q | Phanerodontia chrysosporium | |
11.4 | - |
lactose | pH 4.5, 23°C, mutant enzyme N732D | Phanerodontia chrysosporium | |
16.6 | - |
lactose | pH 4.5, 23°C, mutant enzyme N732E | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenol-indophenol | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellobiose + cytochrome c | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced cytochrome c | - |
? | |
lactose + cytochrome c | lactose binds considerably more weakly than cellobiose | Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Phanerodontia chrysosporium |
cellobiose dehydrogenase | - |
Phanerodontia chrysosporium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme H689V | Phanerodontia chrysosporium | |
0.003 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme H689A | Phanerodontia chrysosporium | |
0.003 | - |
lactose | pH 4.5, 23°C, mutant enzyme H689Q or mutant H689A | Phanerodontia chrysosporium | |
0.004 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme H689N or mutant H689Q | Phanerodontia chrysosporium | |
0.004 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme N732D | Phanerodontia chrysosporium | |
0.005 | - |
lactose | pH 4.5, 23°C, mutant enzyme N732D | Phanerodontia chrysosporium | |
0.009 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme H689E | Phanerodontia chrysosporium | |
0.21 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme N732E | Phanerodontia chrysosporium | |
0.3 | - |
lactose | pH 4.5, 23°C, mutant enzyme N732E | Phanerodontia chrysosporium | |
0.4 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme N732A | Phanerodontia chrysosporium | |
0.7 | - |
lactose | pH 4.5, 23°C, mutant enzyme N732A | Phanerodontia chrysosporium | |
1 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme N732Q | Phanerodontia chrysosporium | |
1.2 | - |
lactose | pH 4.5, 23°C, mutant enzyme N732Q | Phanerodontia chrysosporium | |
1.7 | - |
lactose | pH 4.5, 23°C, mutant enzyme N732H | Phanerodontia chrysosporium | |
2.7 | - |
cellobiose | pH 4.5, 23°C, mutant enzyme N732H | Phanerodontia chrysosporium | |
14.3 | - |
lactose | pH 4.5, 23°C, recombinant wild-type enzyme | Phanerodontia chrysosporium | |
15.5 | - |
cellobiose | pH 4.5, 23°C, recombinant wild-type enzyme | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
cellobiose + cytochrome c, highest activity at pH 3, decreasing as the pH increases, recombinant wild-type enzyme, mutant enzymes N732H, N732Q and N732A | Phanerodontia chrysosporium |
3 | 5 | cellobiose + 2,6-dichlorophenol-indophenol, wild-type enzyme | Phanerodontia chrysosporium |
5 | - |
cellobiose + cytochrome c, mutant enzyme N732E | Phanerodontia chrysosporium |
6 | - |
cellobiose + cytochrome c, mutant enzyme N732D | Phanerodontia chrysosporium |
6.5 | 7 | cellobiose + 2,6-dichlorophenol-indophenol, mutant enzyme N732E and N732D | Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | highest activity at pH 3, decreases when the pH increases to pH 7.0, cellobiose + 2,6-dichlorophenol-indophenol, to about 10% with recombinant wild-type enzyme, to about 25% with mutant enzymes N732Q, to about 40% with mutant enzyme N732A and to about 50% with mutant enzyme N732H | Phanerodontia chrysosporium |
3 | 5.5 | highest activity at pH 3, decreases to 10-20% of the activity when the pH increases to pH 5.5, cellobiose + cytochrome c recombinant wild-type enzyme, mutant enzymes N732H, N732Q and N732A | Phanerodontia chrysosporium |
4 | 6 | pH 5.0: about 35% of maximal activity, pH 7.0: about 20% of maximal activity, cellobiose + cytochrome c, mutant enzyme N732E | Phanerodontia chrysosporium |
5 | 7 | pH 5.0: about 40% of maximal activity, pH 7.0: about 20% of maximal activity, cellobiose + cytochrome c, mutant enzyme N732D | Phanerodontia chrysosporium |
5.5 | 7.5 | pH 5.0: about 45% of maximal activity, pH 7.0: about 65% of maximal activity, cellobiose + 2,6-dichlorophenol-indophenol, mutant enzyme N732E | Phanerodontia chrysosporium |
5.5 | 8 | pH 5.5: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, cellobiose + 2,6-dichlorophenol-indophenol, mutant enzyme N732D | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | the enzyme is an extracellular flavocytochrome | Phanerodontia chrysosporium | |
flavin | the enzyme is an extracellular flavocytochrome | Phanerodontia chrysosporium |