Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.99.18 extracted from

  • Yoshida, M.; Ohira, T.; Igarashi, K.; Nagasawa, H.; Aida, K.; Hallberg, B.M.; Divne, C.; Nishino, T.; samejima, M.
    Production and characterization of recombinant Phanerochaete chrysosporium cellobiose dehydrogenase in the methylotrophic yeast Pichia pastoris (2001), Biosci. Biotechnol. Biochem., 65, 2050-2057.
    View publication on PubMed

Application

Application Comment Organism
biotechnology the Pichia expression system is well suited for high-level production of recombinant enzyme Phanerodontia chrysosporium

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris, enzyme retains catalytic and cellulose-binding properties of the wild-type enzyme Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00126
-
cytochrome c pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium
0.00144
-
cytochrome c pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium
0.0432
-
cellobiose pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium
0.0581
-
cellobiose pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium
0.327
-
ubiquinone pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium
0.333
-
ubiquinone pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
x * 97000, wild-type enzyme, SDS-PAGE Phanerodontia chrysosporium
98000
-
x * 98000, recombinant enzyme, SDS-PAGE Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein MW after deglycosylation is 92000 for the recombinant enzyme and 90000 for wild-type enzyme Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + cytochrome c
-
Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced cytochrome c
-
?
cellobiose + ubiquinone
-
Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced ubiquinone
-
?

Subunits

Subunits Comment Organism
? x * 97000, wild-type enzyme, SDS-PAGE Phanerodontia chrysosporium
? x * 98000, recombinant enzyme, SDS-PAGE Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
35.4
-
cytochrome c pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium
37.3
-
cytochrome c pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium
38.6
-
cellobiose pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium
40
-
cellobiose pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium
43.5
-
ubiquinone pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium
44.2
-
ubiquinone pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
flavin recombinant enzyme contains 0.96 mol of flavin per mol of enzyme, wild-type enzyme contains 0.97 mol of flavin per mol of enzyme Phanerodontia chrysosporium
heme contains one heme cofactor per protein molecule Phanerodontia chrysosporium