Application | Comment | Organism |
---|---|---|
biotechnology | the Pichia expression system is well suited for high-level production of recombinant enzyme | Phanerodontia chrysosporium |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris, enzyme retains catalytic and cellulose-binding properties of the wild-type enzyme | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00126 | - |
cytochrome c | pH 4.0, 30°C, wild-type enzyme | Phanerodontia chrysosporium | |
0.00144 | - |
cytochrome c | pH 4.0, 30°C, recombinant enzyme | Phanerodontia chrysosporium | |
0.0432 | - |
cellobiose | pH 4.0, 30°C, wild-type enzyme | Phanerodontia chrysosporium | |
0.0581 | - |
cellobiose | pH 4.0, 30°C, recombinant enzyme | Phanerodontia chrysosporium | |
0.327 | - |
ubiquinone | pH 4.0, 30°C, recombinant enzyme | Phanerodontia chrysosporium | |
0.333 | - |
ubiquinone | pH 4.0, 30°C, wild-type enzyme | Phanerodontia chrysosporium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
x * 97000, wild-type enzyme, SDS-PAGE | Phanerodontia chrysosporium |
98000 | - |
x * 98000, recombinant enzyme, SDS-PAGE | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | MW after deglycosylation is 92000 for the recombinant enzyme and 90000 for wild-type enzyme | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + cytochrome c | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced cytochrome c | - |
? | |
cellobiose + ubiquinone | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced ubiquinone | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 97000, wild-type enzyme, SDS-PAGE | Phanerodontia chrysosporium |
? | x * 98000, recombinant enzyme, SDS-PAGE | Phanerodontia chrysosporium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35.4 | - |
cytochrome c | pH 4.0, 30°C, wild-type enzyme | Phanerodontia chrysosporium | |
37.3 | - |
cytochrome c | pH 4.0, 30°C, recombinant enzyme | Phanerodontia chrysosporium | |
38.6 | - |
cellobiose | pH 4.0, 30°C, wild-type enzyme | Phanerodontia chrysosporium | |
40 | - |
cellobiose | pH 4.0, 30°C, recombinant enzyme | Phanerodontia chrysosporium | |
43.5 | - |
ubiquinone | pH 4.0, 30°C, wild-type enzyme | Phanerodontia chrysosporium | |
44.2 | - |
ubiquinone | pH 4.0, 30°C, recombinant enzyme | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | recombinant enzyme contains 0.96 mol of flavin per mol of enzyme, wild-type enzyme contains 0.97 mol of flavin per mol of enzyme | Phanerodontia chrysosporium | |
heme | contains one heme cofactor per protein molecule | Phanerodontia chrysosporium |