Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Thermothelomyces myriococcoides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
cellobiose | 2,6-dichloroindophenol as electron acceptor, at 40°C | Thermothelomyces myriococcoides | |
0.14 | - |
lactose | 2,6-diclorindophenol as electron acceptor, at 40°C | Thermothelomyces myriococcoides | |
8.86 | - |
maltose | 2,6-diclorindophenol as electron acceptor, at 40°C | Thermothelomyces myriococcoides | |
21.5 | - |
cytochrome c | lactose as electron donor, at 40°C | Thermothelomyces myriococcoides | |
140.4 | - |
2,6-dichloroindophenol | lactose as electron donor, at 40°C | Thermothelomyces myriococcoides | |
157.1 | - |
glucose | 2,6-diclorindophenol as electron acceptor, at 40°C | Thermothelomyces myriococcoides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
SDS-PAGE | Thermothelomyces myriococcoides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces myriococcoides | - |
- |
- |
Thermothelomyces myriococcoides CBS 208.89 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by hi-trap column and phenyl HIC column chromatography, deglycosylation | Thermothelomyces myriococcoides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.4 | - |
HIC-column purified | Thermothelomyces myriococcoides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
cellobiose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides CBS 208.89 | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
D-glucose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides | ? | - |
? | |
D-glucose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides CBS 208.89 | ? | - |
? | |
lactose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides | ? | - |
? | |
lactose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides CBS 208.89 | ? | - |
? | |
lactose + cytochrome c | - |
Thermothelomyces myriococcoides | ? | - |
? | |
lactose + cytochrome c | - |
Thermothelomyces myriococcoides CBS 208.89 | ? | - |
? | |
maltose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides | ? | - |
? | |
maltose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides CBS 208.89 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cellobiose dehydrogenase | CDH | Thermothelomyces myriococcoides |
MtCDH | - |
Thermothelomyces myriococcoides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
lactose as electron donor, 2,6-diclorindophenol as electron acceptor | Thermothelomyces myriococcoides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | stable for 50 h | Thermothelomyces myriococcoides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
lactose as electron donor, 2,6-diclorindophenol as electron acceptor | Thermothelomyces myriococcoides |
5 | - |
lactose as electron donor, cytochrome c as electron acceptor | Thermothelomyces myriococcoides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | lactose as electron donor, 2,6-diclorindophenol as electron acceptor | Thermothelomyces myriococcoides |