KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.175 | - |
cellobiose | with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor | |
0.285 | - |
cellobiose | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor | |
1.85 | - |
lactose | with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor | |
5.241 | - |
lactose | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
1 * 97000, SDS-PAGE | Cerrena unicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cerrena unicolor | S5R9V1 | - |
- |
Cerrena unicolor FCL139 | S5R9V1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme is glycosylated (8.2%) mainly with mannose and glucosamine residues | Cerrena unicolor |
Purification (Comment) | Organism |
---|---|
ammoinium sulfate precipitation, DEAE-Sepharose column chromatography, Sephadex G-50 gel filtration, and lactose-CPG column chromatography | Cerrena unicolor |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
culture filtrate, at pH 4.5 and 30°C | Cerrena unicolor |
14.4 | - |
after 73.41fold purification, at pH 4.5 and 30°C | Cerrena unicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenolindophenol | - |
Cerrena unicolor | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
cellobiose + 2,6-dichlorophenolindophenol | - |
Cerrena unicolor FCL139 | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
cellobiose + ferricytochrome c | - |
Cerrena unicolor | cellobiono-1,5-lactone + ferrocytochrome c | - |
? | |
cellobiose + ferricytochrome c | - |
Cerrena unicolor FCL139 | cellobiono-1,5-lactone + ferrocytochrome c | - |
? | |
lactose + 2,6-dichlorophenolindophenol | - |
Cerrena unicolor | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + 2,6-dichlorophenolindophenol | - |
Cerrena unicolor FCL139 | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + ferricytochrome c | - |
Cerrena unicolor | lactono-1,5-lactone + ferrocytochrome c | - |
? | |
lactose + ferricytochrome c | - |
Cerrena unicolor FCL139 | lactono-1,5-lactone + ferrocytochrome c | - |
? | |
additional information | the enzyme is unable to oxidize glucose | Cerrena unicolor | ? | - |
? | |
additional information | the enzyme is unable to oxidize glucose | Cerrena unicolor FCL139 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 97000, SDS-PAGE | Cerrena unicolor |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Cerrena unicolor |
CDH1 | - |
Cerrena unicolor |
cellobiose [acceptor] 1-oxidoreductase | - |
Cerrena unicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Cerrena unicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | complete loss of enzyme activity is recorded at 90°C after heat exposure for 30 min and at 50°C after 12 h | Cerrena unicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.86 | - |
cellobiose | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor | |
19.06 | - |
cellobiose | with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor | |
19.46 | - |
lactose | with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor | |
20.86 | - |
lactose | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Cerrena unicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6 | more than 50% activity between pH 3.4 and 6.0 | Cerrena unicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 9 | the enzyme is stable for 12 h at pH 3.0-6.0, losing 50% of its initial activity within approximately 3 h at pH 2.0 and 8.0. The enzyme is especially sensitive to pH values above 9.0 | Cerrena unicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Cerrena unicolor | |
heme b | - |
Cerrena unicolor |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cerrena unicolor | isoelectric focusing | - |
4.6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.98 | - |
lactose | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor | |
10.52 | - |
lactose | with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor | |
66.18 | - |
cellobiose | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor | |
108.9 | - |
cellobiose | with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C | Cerrena unicolor |