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Literature summary for 1.1.99.18 extracted from

  • Geiss, A.; Reichhart, T.; Pejker, B.; Plattner, E.; Herzog, P.; Schulz, C.; Ludwig, R.; Felice, A.; Haltrich, D.
    Engineering the turnover stability of cellobiose dehydrogenase toward long-term bioelectronic applications (2021), ACS Sust. Chem. Eng., 9, 7086-7100 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.18

Application

Application Comment Organism
biofuel production attractive oxidoreductase for bioelectrochemical applications. Its two-domain structure allows the flavoheme enzyme to establish direct electron transfer to biosensor and biofuel cell electrodes. The application of CDH in these devices is impeded by its limited stability under turnover conditions. Screening results in one CDH variant that exhibits improved turnover stability on a biosensor electrode, which is suitable for the application in implantable continuous glucose monitoring biosensors or biofuel cells Thermothelomyces myriococcoides

Protein Variants

Protein Variants Comment Organism
L324C the variant loses approximately half of their activities within 52 h at 37°C Thermothelomyces myriococcoides
L324C/M409S lower catalytic activity as compared to wild-type enzyme. The variant loses approximately half of their activities within 52 h at 37°C Thermothelomyces myriococcoides
M309K retains most of its initial activity among the single variants. Mutation improve the turnover stability of the enzyme Thermothelomyces myriococcoides
M309K/L324C the Tm-value of the variant is 1.8°C lower than the wild-type value Thermothelomyces myriococcoides
M309K/M409S variant with the highest residual activity after 4 h at 41 °C under turnover conditions. Lower catalytic activity as compared to wild-type enzyme Thermothelomyces myriococcoides
M309V the mutant enzyme shows significant stability improvements compared to the wild-type enzyme. Higher cytochrome c/2,6-dichloroindophenol activity ratio than the wild-type enzyme Thermothelomyces myriococcoides
M409S mutation improve the turnover stability of the enzyme. Most stable variant with an electrode half-life of 13.5 h Thermothelomyces myriococcoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 cytochrome c pH 7.4, 37°C, mutant enzyme M309K/L324C Thermothelomyces myriococcoides
9
-
 cytochrome c pH 7.4, 37°C, mutant enzyme M309K/M409S Thermothelomyces myriococcoides
11
-
 cytochrome c pH 7.4, 37°C, mutant enzyme L324C/M409S Thermothelomyces myriococcoides
41
-
 cytochrome c pH 7.4, 37°C, mutant enzyme M409S Thermothelomyces myriococcoides
44
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme M309K/M409S Thermothelomyces myriococcoides
72
-
 cytochrome c pH 7.4, 37°C, mutant enzyme M309K Thermothelomyces myriococcoides
81
-
 cytochrome c pH 7.4, 37°C, wild-type enzyme Thermothelomyces myriococcoides
84
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme M409S Thermothelomyces myriococcoides
118
-
 cytochrome c pH 7.4, 37°C, mutant enzyme L324C Thermothelomyces myriococcoides
130
-
 cytochrome c pH 7.4, 37°C, mutant enzyme M309V Thermothelomyces myriococcoides
138
-
 2,6-dichloroindophenol pH 7.4, 37°C, wild-type enzyme Thermothelomyces myriococcoides
196
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme M309V Thermothelomyces myriococcoides
200
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme M309K Thermothelomyces myriococcoides
205
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme L324C Thermothelomyces myriococcoides
219
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme L324C/M409S Thermothelomyces myriococcoides
350
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme M309K/L324C Thermothelomyces myriococcoides

Organism

Organism UniProt Comment Textmining
Thermothelomyces myriococcoides A9XK88
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucose + 2,6-dichloroindophenol
-
 Thermothelomyces myriococcoides ? + reduced 2,6-dichloroindophenol
-
 ?
glucose + cytochrome c
-
 Thermothelomyces myriococcoides ? + reduced cytochrome c
-
 ?

Synonyms

Synonyms Comment Organism
Cdh
-
 Thermothelomyces myriococcoides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 residual activity after 52 h when stored in 11 mM PBS pH 7.4 buffer: wild-type enzyme (106%), mutant enzyme M309V: (103%), mutant enzyme M309K: (94%), mutant enzyme M409S: (102%), mutant enzyme M309K/M409S: (116%), mutant enzyme L324C: (57%), mutant enzyme L324C/M409S: (58%), mutant enzyme M309K/L324C: (8%) Thermothelomyces myriococcoides
47.6
-
 Tm-value, mutant enzyme M309K/M409S Thermothelomyces myriococcoides
49.8
-
 Tm-value, mutant enzyme M409S Thermothelomyces myriococcoides
51.9
-
 Tm-value, mutant enzyme L324C/M409S Thermothelomyces myriococcoides
53.9
-
 Tm-value, mutant enzyme M309K Thermothelomyces myriococcoides
55
-
 Tm-value, mutant enzyme M309K/L324C Thermothelomyces myriococcoides
56.3
-
 Tm-value, mutant enzyme L324C Thermothelomyces myriococcoides
56.4
-
 Tm-value, mutant enzyme M309V Thermothelomyces myriococcoides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 cytochrome c pH 7.4, 37°C, mutant enzyme M309K/M409S Thermothelomyces myriococcoides
1.2
-
 cytochrome c pH 7.4, 37°C, mutant enzyme L324C/M409S Thermothelomyces myriococcoides
1.2
-
 cytochrome c pH 7.4, 37°C, mutant enzyme M309K/L324C Thermothelomyces myriococcoides
1.6
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme M309K/M409S Thermothelomyces myriococcoides
3
-
 cytochrome c pH 7.4, 37°C, mutant enzyme M409S Thermothelomyces myriococcoides
3.1
-
 cytochrome c pH 7.4, 37°C, mutant enzyme L324C Thermothelomyces myriococcoides
3.1
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme M309K/L324C Thermothelomyces myriococcoides
3.7
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme L324C/M409S Thermothelomyces myriococcoides
4.2
-
 cytochrome c pH 7.4, 37°C, mutant enzyme M309V Thermothelomyces myriococcoides
6.2
-
 cytochrome c pH 7.4, 37°C, mutant enzyme M309K Thermothelomyces myriococcoides
6.3
-
 cytochrome c pH 7.4, 37°C, wild-type enzyme Thermothelomyces myriococcoides
7.1
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme M409S Thermothelomyces myriococcoides
8.6
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme L324C Thermothelomyces myriococcoides
13
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme M309K Thermothelomyces myriococcoides
15.1
-
 2,6-dichloroindophenol pH 7.4, 37°C, mutant enzyme M309V Thermothelomyces myriococcoides
17.4
-
 2,6-dichloroindophenol pH 7.4, 37°C, wild-type enzyme Thermothelomyces myriococcoides