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Literature summary for 1.1.99.18 extracted from
- Transformation of cellobiose during the interaction of cellobiose dehydrogenase and beta-glucosidase of Cerrena unicolor (2018), J. Basic Microbiol., 58, 322-330 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
cellobiose | pH 5.5, 30°C | Cerrena unicolor |  |
| 25.2 | - |
lactose | pH 5.5, 30°C | Cerrena unicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
gel filtration | Cerrena unicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cerrena unicolor | S5R9V1 | - |
- |
| Cerrena unicolor VKM F-3196 | S5R9V1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cerrena unicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.7 | - |
pH 5.5, 30°C | Cerrena unicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + 2,6-dichloroindophenol | with 2,6-dichloroindophenol as acceptor the enzyme catalyzes the oxidation of cellobiose more efficiently than lactose | Cerrena unicolor | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
| cellobiose + 2,6-dichloroindophenol | with 2,6-dichloroindophenol as acceptor the enzyme catalyzes the oxidation of cellobiose more efficiently than lactose | Cerrena unicolor VKM F-3196 | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
| lactose + 2,6-dichloroindophenol | - |
Cerrena unicolor | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
| lactose + 2,6-dichloroindophenol | - |
Cerrena unicolor VKM F-3196 | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 95000, SDS-PAGE | Cerrena unicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cdh | - |
Cerrena unicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Cerrena unicolor |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | 25°C: about 85% of maximal activity, 55°C: about 65% of maximal activity | Cerrena unicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
the enzyme maintains an activity at 70°C with k(app) = 0.163/min | Cerrena unicolor |
| 80 | - |
1 min, inactivated | Cerrena unicolor |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.8 | - |
lactose | pH 5.5, 30°C | Cerrena unicolor | |
| 15.2 | - |
cellobiose | pH 5.5, 30°C | Cerrena unicolor | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Cerrena unicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7 | pH 3.5: about 35% of maximal activity, pH 7.0: about 50% of maximal activity | Cerrena unicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | the enzyme retains more than 90% of activity at pH below 6 and more than 50% activity at pH 10 | Cerrena unicolor |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Cerrena unicolor | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the interaction of cellobiose dehydrogenase (CDH) and beta-GLU (beta-glucosidase) determines the conversion of cellobiose to cellobionolactone or glucose. The formation of cellobionolactone from cellobiose as a result of the action of CDH depends on the availability of quinones (for CDH), which are formed by the interaction of laccase with a phenolic compound. At low quinone concentrations, cellobiose is transformed by beta-GLU to glucose | Cerrena unicolor |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.25 | - |
lactose | pH 5.5, 30°C | Cerrena unicolor | |
| 49 | - |
cellobiose | pH 5.5, 30°C | Cerrena unicolor | |
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