BRENDA - Enzyme Database show
show all sequences of 1.1.99.20

Inducible or constitutive polyethylene glycol dehydrogenase involved in the aerobic metabolism of polyethylene glycol

Yamanaka, H.; Kawai, F.; J. Ferment. Bioeng. 67, 300-302 (1989)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ethylamine
30% activation, Pseudomonas sp. strain No. 43
Pseudomonas sp.
methylamine
30% activation, Pseudomonas sp. strain No. 43
Pseudomonas sp.
phenazine methosulfate
activation, accelerates dichlorophenolindophenol reduction
Flavobacterium sp.
phenazine methosulfate
activation, accelerates dichlorophenolindophenol reduction
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,4-benzoquinone
at 0.02-0.05 mM, strong inhibitor; competitive to 2,6-dichlorophenolindophenol
Flavobacterium sp.
1,4-benzoquinone
at 0.02-0.05 mM, strong inhibitor; competitive to 2,6-dichlorophenolindophenol
Pseudomonas sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pseudomonas sp.
16020
-
membrane
-
Flavobacterium sp.
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
30% activation, Pseudomonas sp. strain No. 101
Pseudomonas sp.
additional information
no metal ion requirement
Flavobacterium sp.
additional information
no metal ion requirement
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Flavobacterium sp.
-
strains No.202 to 205; synergistic mixed culture with Pseudomonas sp.
-
Pseudomonas sp.
-
strains No.42, 43, 101, 103; synergistic mixed culture with Flavobacterium sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.028
-
strain No. 43
Pseudomonas sp.
0.035
-
strain No. 203, crude extract
Flavobacterium sp.
0.164
-
strain No. 103
Pseudomonas sp.
1.2
-
mixed culture
Flavobacterium sp.
1.2
-
mixed culture
Pseudomonas sp.
2.68
-
strain No. 203, purified enzyme
Flavobacterium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
PEG-1000 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-1000 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-200 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-200 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-20000 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-20000 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-300 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-300 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-400 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-400 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-4000 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-4000 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-6000 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-6000 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
polyethylene glycol + acceptor
constitutive quinoprotein enzyme, enzyme of Flavobacterium sp. No.203 inductive
389907
Flavobacterium sp.
?
-
-
-
?
polyethylene glycol + acceptor
first and main step in aerobic PEG-metabolism
389907
Pseudomonas sp.
?
-
-
-
?
polyethylene glycol + acceptor
first and main step in aerobic PEG-metabolism
389907
Flavobacterium sp.
?
-
-
-
?
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389907
Pseudomonas sp.
aldehyde + reduced acceptor
-
389907
Pseudomonas sp.
?
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389907
Flavobacterium sp.
aldehyde + reduced acceptor
-
389907
Flavobacterium sp.
?
Cofactor
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
electron acceptor
Flavobacterium sp.
2,6-dichlorophenolindophenol
electron acceptor
Pseudomonas sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ethylamine
30% activation, Pseudomonas sp. strain No. 43
Pseudomonas sp.
methylamine
30% activation, Pseudomonas sp. strain No. 43
Pseudomonas sp.
phenazine methosulfate
activation, accelerates dichlorophenolindophenol reduction
Flavobacterium sp.
phenazine methosulfate
activation, accelerates dichlorophenolindophenol reduction
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
electron acceptor
Flavobacterium sp.
2,6-dichlorophenolindophenol
electron acceptor
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,4-benzoquinone
at 0.02-0.05 mM, strong inhibitor; competitive to 2,6-dichlorophenolindophenol
Flavobacterium sp.
1,4-benzoquinone
at 0.02-0.05 mM, strong inhibitor; competitive to 2,6-dichlorophenolindophenol
Pseudomonas sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pseudomonas sp.
16020
-
membrane
-
Flavobacterium sp.
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
30% activation, Pseudomonas sp. strain No. 101
Pseudomonas sp.
additional information
no metal ion requirement
Flavobacterium sp.
additional information
no metal ion requirement
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.028
-
strain No. 43
Pseudomonas sp.
0.035
-
strain No. 203, crude extract
Flavobacterium sp.
0.164
-
strain No. 103
Pseudomonas sp.
1.2
-
mixed culture
Flavobacterium sp.
1.2
-
mixed culture
Pseudomonas sp.
2.68
-
strain No. 203, purified enzyme
Flavobacterium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
PEG-1000 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-1000 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-200 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-200 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-20000 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-20000 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-300 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-300 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-400 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-400 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-4000 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-4000 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
PEG-6000 + acceptor
-
389907
Pseudomonas sp.
?
-
-
-
?
PEG-6000 + acceptor
-
389907
Flavobacterium sp.
?
-
-
-
?
polyethylene glycol + acceptor
constitutive quinoprotein enzyme, enzyme of Flavobacterium sp. No.203 inductive
389907
Flavobacterium sp.
?
-
-
-
?
polyethylene glycol + acceptor
first and main step in aerobic PEG-metabolism
389907
Pseudomonas sp.
?
-
-
-
?
polyethylene glycol + acceptor
first and main step in aerobic PEG-metabolism
389907
Flavobacterium sp.
?
-
-
-
?
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389907
Pseudomonas sp.
aldehyde + reduced acceptor
-
389907
Pseudomonas sp.
?
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389907
Flavobacterium sp.
aldehyde + reduced acceptor
-
389907
Flavobacterium sp.
?
Other publictions for EC 1.1.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725161
Liu
Catalytic mechanism of short e ...
Ensifer sp. AS08
Int. J. Mol. Sci.
14
1218-1231
2013
-
-
1
-
5
-
-
6
-
-
-
-
-
3
-
-
1
-
-
-
-
-
3
-
-
-
-
6
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
671546
Hu
Isolation of bacteria able to ...
Sphingopyxis macrogoltabida
Appl. Microbiol. Biotechnol.
73
1407-1413
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688864
Tani
Structure and conservation of ...
Sphingopyxis macrogoltabida 103, Sphingopyxis macrogoltabida, Sphingopyxis macrogoltabida 203, Sphingopyxis terrae
Microbiology
153
338-346
2007
-
2
2
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671461
Ohta
Analysis of amino acid residue ...
Sphingopyxis terrae
Appl. Environ. Microbiol.
72
4388-4396
2006
-
-
-
-
-
-
-
21
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389911
Sugimoto
The first step in polyethylene ...
Sphingopyxis terrae
J. Bacteriol.
183
6694-6698
2001
-
-
1
-
-
-
-
10
1
-
2
-
-
7
-
-
1
-
-
-
-
-
12
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
10
1
-
2
-
-
-
-
1
-
-
-
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389910
Yasuda
-
Polyethylene glycol dehydrogen ...
Rhodoblastus acidophilus, Rhodoblastus acidophilus M402
FEMS Microbiol. Lett.
138
23-28
1996
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389907
Yamanaka
-
Inducible or constitutive poly ...
Flavobacterium sp., Pseudomonas sp.
J. Ferment. Bioeng.
67
300-302
1989
4
-
-
-
-
-
2
-
2
3
-
-
-
2
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
2
-
-
-
4
-
-
2
-
-
-
-
2
-
-
2
3
-
-
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389908
Yamanaka
-
Purification and characterizat ...
Flavobacterium sp.
J. Ferment. Bioeng.
67
324-330
1989
1
-
-
-
-
-
14
4
1
-
2
-
-
1
-
-
1
-
-
-
2
-
36
1
1
1
2
-
2
1
1
5
-
-
-
1
-
-
5
-
-
-
-
14
-
4
1
-
2
-
-
-
-
1
-
-
2
-
36
1
1
1
2
-
2
1
1
-
-
-
-
-
-
-
389909
Kawai
Biodegradation of polyethylene ...
Flavobacterium sp., Pseudomonas sp.
Arch. Microbiol.
146
125-129
1986
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389904
Kawai
Identification of the prosthet ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
49
1071-1076
1985
4
-
-
-
-
-
8
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
10
-
-
-
4
-
-
10
-
-
-
-
8
-
4
2
-
-
-
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389905
Kawai
-
Identification of reaction pro ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
47
1669-1671
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389906
Kawai
Purification and characterizat ...
Flavobacterium sp., Pseudomonas sp.
Appl. Environ. Microbiol.
40
701-705
1980
-
-
-
-
-
-
22
4
-
4
4
-
-
3
-
-
2
-
-
-
2
-
28
2
2
2
4
-
2
2
6
2
-
-
-
-
-
-
2
-
-
-
-
22
-
4
-
4
4
-
-
-
-
2
-
-
2
-
28
2
2
2
4
-
2
2
6
-
-
-
-
-
-
-