BRENDA - Enzyme Database
show all sequences of 1.1.99.20

Purification and characterization of constitutive polyethylene glycol (PEG) dehydrogenase of a PEG 4000-utilizing Flavobacterium sp. No. 203

Yamanaka, H.; Kawai, F.; J. Ferment. Bioeng. 67, 324-330 (1989)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Flavobacterium sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,4-benzoquinone
at 0.01-1 mM inhibitor; competitive to 2,6-dichlorophenolindophenol
Flavobacterium sp.
arsenite
-
Flavobacterium sp.
Cd2+
-
Flavobacterium sp.
Co2+
-
Flavobacterium sp.
Cu2+
at 1 mM; competitive
Flavobacterium sp.
Fe3+
-
Flavobacterium sp.
Hg2+
at 1 mM; competitive
Flavobacterium sp.
hydrazine
weak
Flavobacterium sp.
hydroxylamine
weak
Flavobacterium sp.
Mn2+
-
Flavobacterium sp.
monoiodoacetate
weak
Flavobacterium sp.
Ni2+
-
Flavobacterium sp.
p-chloromercuribenzoate
-
Flavobacterium sp.
Semicarbazide
weak
Flavobacterium sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
PEG-400
-
Flavobacterium sp.
1.7
-
PEG-1000
-
Flavobacterium sp.
2.8
-
PEG-4000
-
Flavobacterium sp.
5.9
-
PEG-6000
-
Flavobacterium sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Flavobacterium sp.
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
4 * 57000, Flavobacterium sp. No.203, SDS-PAGE
Flavobacterium sp.
220000
-
strain No.203, gel filtration
Flavobacterium sp.
Organism
Organism
UniProt
Commentary
Textmining
Flavobacterium sp.
-
strains No.202 to 205
-
Purification (Commentary)
Purification (Commentary)
Organism
strain No. 203, by precipitation with ammonium sulfate, solubilization with benzalkonium chloride, chromatography with DEAE-Toyopearl 650M and hydroxylapatite and gel filtration on Toyopearl HW-55
Flavobacterium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Flavobacterium sp.
2.59
-
-
Flavobacterium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-dodecanol + acceptor
-
389908
Flavobacterium sp.
n-dodecanal + reduced acceptor
-
389908
Flavobacterium sp.
?
1-hexanol + acceptor
-
389908
Flavobacterium sp.
n-hexanal + reduced acceptor
-
389908
Flavobacterium sp.
?
1-octanol + acceptor
-
389908
Flavobacterium sp.
n-octanal + reduced acceptor
-
389908
Flavobacterium sp.
?
2-carboxybenzaldehyde + acceptor
-
389908
Flavobacterium sp.
2-carboxybenzoic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
acetaldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
acetic acid + reduced acceptor
-
389908
Flavobacterium sp.
-
aliphatic aldehyde + acceptor + H2O
C-3 to C-7
389908
Flavobacterium sp.
carboxylic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
aromatic alcohol + acceptor
-
389908
Flavobacterium sp.
aromatic aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
aromatic aldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
aromatic carboxylic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
benzaldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
benzoic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
benzyl alcohol + acceptor
-
389908
Flavobacterium sp.
benzaldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
beta-phenethyl alcohol + acceptor
-
389908
Flavobacterium sp.
beta-phenethyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
cinnamyl alcohol + acceptor
-
389908
Flavobacterium sp.
cinnamyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
diethyleneglycol monoethylether
-
389908
Flavobacterium sp.
?
-
-
-
?
diols + acceptor
except ethylene glycol, 1,2-and 1,3-propenediol
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
diols + acceptor
diethylene glycol and triethylene glycol are only poor substrates
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
diols + acceptor
overview: with primary alcoholic groups
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
ethylene glycol monoethylether
-
389908
Flavobacterium sp.
?
-
-
-
?
ethyleneglycol monophenylether
-
389908
Flavobacterium sp.
?
-
-
-
?
ethyleneglycolmono-n-butylether
-
389908
Flavobacterium sp.
?
-
-
-
?
additional information
the enzyme shows no activity toward ethylene glycol, glycerol, secondary alcohols and sugar alcohols
389908
Flavobacterium sp.
?
-
-
-
-
PEG-1000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-200 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-20000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-300 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-400 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-4000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-6000 + acceptor
most active substrate of all PEGs
389908
Flavobacterium sp.
?
-
-
-
?
polyethylene glycol + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
primary alcohol + acceptor
C-3 to C-12
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
primary alcohol + acceptor
methanol, glycerol, secondary alcohols and sugar alcohols are no substrates
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
primary alcohol + acceptor
coenzyme Q1 and Q2 can act as acceptors
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
tetraethylene glycol + acceptor
-
389908
Flavobacterium sp.
tetraethylene glycol aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
trans-cinnamaldehyde + acceptor
-
389908
Flavobacterium sp.
trans-cinnamic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
vanillin aldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
vanillic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
vannilyl alcohol + acceptor
-
389908
Flavobacterium sp.
vannilyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 57000, Flavobacterium sp. No.203, SDS-PAGE
Flavobacterium sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Flavobacterium sp.
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
18
50
about half-maximal activity at 18°C and 50°C
Flavobacterium sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable below
Flavobacterium sp.
60
-
78% loss of activity
Flavobacterium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Flavobacterium sp.
8
-
-
Flavobacterium sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
about 75% of maximal activity at pH 6.5 and pH 8.5
Flavobacterium sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
8
stable
Flavobacterium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
electron acceptor
Flavobacterium sp.
coenzyme Q1
electron acceptor
Flavobacterium sp.
coenzyme Q2
electron acceptor
Flavobacterium sp.
additional information
nitroblue tetrazolium, cytochrome c, ferricyanide, NADP+, NAD+, and coenzyme Q6 and Q10 do not serve as electron acceptors
Flavobacterium sp.
pyrroloquinoline quinone
prosthetic group, noncovalently bound to the enzyme
Flavobacterium sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Flavobacterium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
electron acceptor
Flavobacterium sp.
coenzyme Q1
electron acceptor
Flavobacterium sp.
coenzyme Q2
electron acceptor
Flavobacterium sp.
additional information
nitroblue tetrazolium, cytochrome c, ferricyanide, NADP+, NAD+, and coenzyme Q6 and Q10 do not serve as electron acceptors
Flavobacterium sp.
pyrroloquinoline quinone
prosthetic group, noncovalently bound to the enzyme
Flavobacterium sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,4-benzoquinone
at 0.01-1 mM inhibitor; competitive to 2,6-dichlorophenolindophenol
Flavobacterium sp.
arsenite
-
Flavobacterium sp.
Cd2+
-
Flavobacterium sp.
Co2+
-
Flavobacterium sp.
Cu2+
at 1 mM; competitive
Flavobacterium sp.
Fe3+
-
Flavobacterium sp.
Hg2+
at 1 mM; competitive
Flavobacterium sp.
hydrazine
weak
Flavobacterium sp.
hydroxylamine
weak
Flavobacterium sp.
Mn2+
-
Flavobacterium sp.
monoiodoacetate
weak
Flavobacterium sp.
Ni2+
-
Flavobacterium sp.
p-chloromercuribenzoate
-
Flavobacterium sp.
Semicarbazide
weak
Flavobacterium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
PEG-400
-
Flavobacterium sp.
1.7
-
PEG-1000
-
Flavobacterium sp.
2.8
-
PEG-4000
-
Flavobacterium sp.
5.9
-
PEG-6000
-
Flavobacterium sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Flavobacterium sp.
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
4 * 57000, Flavobacterium sp. No.203, SDS-PAGE
Flavobacterium sp.
220000
-
strain No.203, gel filtration
Flavobacterium sp.
Purification (Commentary) (protein specific)
Commentary
Organism
strain No. 203, by precipitation with ammonium sulfate, solubilization with benzalkonium chloride, chromatography with DEAE-Toyopearl 650M and hydroxylapatite and gel filtration on Toyopearl HW-55
Flavobacterium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Flavobacterium sp.
2.59
-
-
Flavobacterium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-dodecanol + acceptor
-
389908
Flavobacterium sp.
n-dodecanal + reduced acceptor
-
389908
Flavobacterium sp.
?
1-hexanol + acceptor
-
389908
Flavobacterium sp.
n-hexanal + reduced acceptor
-
389908
Flavobacterium sp.
?
1-octanol + acceptor
-
389908
Flavobacterium sp.
n-octanal + reduced acceptor
-
389908
Flavobacterium sp.
?
2-carboxybenzaldehyde + acceptor
-
389908
Flavobacterium sp.
2-carboxybenzoic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
acetaldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
acetic acid + reduced acceptor
-
389908
Flavobacterium sp.
-
aliphatic aldehyde + acceptor + H2O
C-3 to C-7
389908
Flavobacterium sp.
carboxylic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
aromatic alcohol + acceptor
-
389908
Flavobacterium sp.
aromatic aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
aromatic aldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
aromatic carboxylic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
benzaldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
benzoic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
benzyl alcohol + acceptor
-
389908
Flavobacterium sp.
benzaldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
beta-phenethyl alcohol + acceptor
-
389908
Flavobacterium sp.
beta-phenethyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
cinnamyl alcohol + acceptor
-
389908
Flavobacterium sp.
cinnamyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
diethyleneglycol monoethylether
-
389908
Flavobacterium sp.
?
-
-
-
?
diols + acceptor
except ethylene glycol, 1,2-and 1,3-propenediol
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
diols + acceptor
diethylene glycol and triethylene glycol are only poor substrates
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
diols + acceptor
overview: with primary alcoholic groups
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
ethylene glycol monoethylether
-
389908
Flavobacterium sp.
?
-
-
-
?
ethyleneglycol monophenylether
-
389908
Flavobacterium sp.
?
-
-
-
?
ethyleneglycolmono-n-butylether
-
389908
Flavobacterium sp.
?
-
-
-
?
additional information
the enzyme shows no activity toward ethylene glycol, glycerol, secondary alcohols and sugar alcohols
389908
Flavobacterium sp.
?
-
-
-
-
PEG-1000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-200 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-20000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-300 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-400 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-4000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
PEG-6000 + acceptor
most active substrate of all PEGs
389908
Flavobacterium sp.
?
-
-
-
?
polyethylene glycol + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
primary alcohol + acceptor
C-3 to C-12
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
primary alcohol + acceptor
methanol, glycerol, secondary alcohols and sugar alcohols are no substrates
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
primary alcohol + acceptor
coenzyme Q1 and Q2 can act as acceptors
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
tetraethylene glycol + acceptor
-
389908
Flavobacterium sp.
tetraethylene glycol aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
trans-cinnamaldehyde + acceptor
-
389908
Flavobacterium sp.
trans-cinnamic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
vanillin aldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
vanillic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
vannilyl alcohol + acceptor
-
389908
Flavobacterium sp.
vannilyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 57000, Flavobacterium sp. No.203, SDS-PAGE
Flavobacterium sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Flavobacterium sp.
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
18
50
about half-maximal activity at 18°C and 50°C
Flavobacterium sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable below
Flavobacterium sp.
60
-
78% loss of activity
Flavobacterium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Flavobacterium sp.
8
-
-
Flavobacterium sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
about 75% of maximal activity at pH 6.5 and pH 8.5
Flavobacterium sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
8
stable
Flavobacterium sp.
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Liu
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1
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5
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6
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-
-
4
-
-
1
-
-
-
-
-
3
-
3
-
-
-
6
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
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6
-
-
-
-
-
-
-
1
-
-
-
-
3
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-
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-
-
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6
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-
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-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688864
Tani
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2
2
-
-
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-
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5
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8
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6
-
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-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671461
Ohta
Analysis of amino acid residue ...
Sphingopyxis terrae
Appl. Environ. Microbiol.
72
4388-4396
2006
-
-
-
-
-
-
-
21
-
-
-
-
-
6
-
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389911
Sugimoto
The first step in polyethylene ...
Sphingopyxis terrae
J. Bacteriol.
183
6694-6698
2001
-
-
1
-
-
-
-
10
1
-
2
-
-
7
-
-
1
-
-
-
-
-
12
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
10
1
-
2
-
-
-
-
1
-
-
-
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389910
Yasuda
-
Polyethylene glycol dehydrogen ...
Rhodoblastus acidophilus, Rhodoblastus acidophilus M402
FEMS Microbiol. Lett.
138
23-28
1996
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389907
Yamanaka
-
Inducible or constitutive poly ...
Flavobacterium sp., Pseudomonas sp.
J. Ferment. Bioeng.
67
300-302
1989
4
-
-
-
-
-
2
-
2
3
-
-
-
2
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
-
2
-
-
-
4
-
-
2
-
-
-
-
2
-
-
2
3
-
-
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389908
Yamanaka
-
Purification and characterizat ...
Flavobacterium sp.
J. Ferment. Bioeng.
67
324-330
1989
1
-
-
-
-
-
14
4
1
-
2
-
-
1
-
-
1
-
-
-
2
-
36
1
-
1
1
2
-
2
1
1
5
-
-
-
1
-
-
5
-
-
-
-
14
-
4
1
-
2
-
-
-
-
1
-
-
2
-
36
1
1
1
2
-
2
1
1
-
-
-
-
-
-
-
389909
Kawai
Biodegradation of polyethylene ...
Flavobacterium sp., Pseudomonas sp.
Arch. Microbiol.
146
125-129
1986
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389904
Kawai
-
Identification of the prosthet ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
49
1071-1076
1985
4
-
-
-
-
-
8
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
-
10
-
-
-
4
-
-
10
-
-
-
-
8
-
4
2
-
-
-
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389905
Kawai
-
Identification of reaction pro ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
47
1669-1671
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389906
Kawai
Purification and characterizat ...
Flavobacterium sp., Pseudomonas sp.
Appl. Environ. Microbiol.
40
701-705
1980
-
-
-
-
-
-
22
4
-
4
4
-
-
3
-
-
2
-
-
-
2
-
28
2
-
2
2
4
-
2
2
6
2
-
-
-
-
-
-
2
-
-
-
-
22
-
4
-
4
4
-
-
-
-
2
-
-
2
-
28
2
2
2
4
-
2
2
6
-
-
-
-
-
-
-