BRENDA - Enzyme Database show
show all sequences of 1.1.99.20

The first step in polyethylene glycol degradation by Sphingomonads proceeds via a flavoprotein alcohol dehydrogenase containing flavin adenine dinucleotide

Sugimoto, M.; Tanabe, M.; Hataya, M.; Enokibara, S.; Duine, J.A.; Kawai, F.; J. Bacteriol. 183, 6694-6698 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene cloned, sequenced and expressed in Escherichia coli
Sphingopyxis terrae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
1-Heptanol
-
Sphingopyxis terrae
0.5
-
1-Hexanol
-
Sphingopyxis terrae
0.6
-
benzyl alcohol
-
Sphingopyxis terrae
0.7
-
PEG-400
-
Sphingopyxis terrae
0.8
-
PEG-300
-
Sphingopyxis terrae
1.6
-
PEG-1000
-
Sphingopyxis terrae
2.4
-
PEG-6000
-
Sphingopyxis terrae
4.2
-
tetraethylene glycol-monoethyl ether
-
Sphingopyxis terrae
6.6
-
1-propanol
-
Sphingopyxis terrae
14
-
Tetraethylene glycol
-
Sphingopyxis terrae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Sphingopyxis terrae
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58800
-
2 * 58800, SDS-PAGE
Sphingopyxis terrae
120000
-
gel filtration
Sphingopyxis terrae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingopyxis terrae
-
-
-
Purification (Commentary)
Commentary
Organism
from a symbiotic mixed culture E-1, consisting of Sphingomonas terrae and a Rhizobium sp.
Sphingopyxis terrae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-heptanol + acceptor
-
389911
Sphingopyxis terrae
n-heptanal + reduced acceptor
-
389911
Sphingopyxis terrae
?
1-hexanol + acceptor
-
389911
Sphingopyxis terrae
n-hexanal + reduced acceptor
-
389911
Sphingopyxis terrae
?
aromatic alcohol + acceptor
-
389911
Sphingopyxis terrae
aromatic aldehyde + reduced acceptor
-
389911
Sphingopyxis terrae
?
benzyl alcohol + acceptor
benzyl alcohol is the best substrate
389911
Sphingopyxis terrae
benzaldehyde + reduced acceptor
-
389911
Sphingopyxis terrae
?
PEG-1000 + acceptor
-
389911
Sphingopyxis terrae
?
-
-
-
?
PEG-300 + acceptor
-
389911
Sphingopyxis terrae
?
-
-
-
?
PEG-400 + acceptor
-
389911
Sphingopyxis terrae
?
-
-
-
?
PEG-6000 + acceptor
-
389911
Sphingopyxis terrae
?
-
-
-
?
polyethylene glycol + acceptor
-
389911
Sphingopyxis terrae
?
-
-
-
?
primary alcohol + acceptor
-
389911
Sphingopyxis terrae
aldehyde + reduced acceptor
-
389911
Sphingopyxis terrae
?
tetraethylene glycol + acceptor
-
389911
Sphingopyxis terrae
tetraethylene glycol aldehyde + reduced acceptor
-
389911
Sphingopyxis terrae
?
tetraethyleneglycol monoethyl ether
-
389911
Sphingopyxis terrae
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 58800, SDS-PAGE
Sphingopyxis terrae
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Sphingopyxis terrae
Cloned(Commentary) (protein specific)
Commentary
Organism
gene cloned, sequenced and expressed in Escherichia coli
Sphingopyxis terrae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Sphingopyxis terrae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
1-Heptanol
-
Sphingopyxis terrae
0.5
-
1-Hexanol
-
Sphingopyxis terrae
0.6
-
benzyl alcohol
-
Sphingopyxis terrae
0.7
-
PEG-400
-
Sphingopyxis terrae
0.8
-
PEG-300
-
Sphingopyxis terrae
1.6
-
PEG-1000
-
Sphingopyxis terrae
2.4
-
PEG-6000
-
Sphingopyxis terrae
4.2
-
tetraethylene glycol-monoethyl ether
-
Sphingopyxis terrae
6.6
-
1-propanol
-
Sphingopyxis terrae
14
-
Tetraethylene glycol
-
Sphingopyxis terrae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Sphingopyxis terrae
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58800
-
2 * 58800, SDS-PAGE
Sphingopyxis terrae
120000
-
gel filtration
Sphingopyxis terrae
Purification (Commentary) (protein specific)
Commentary
Organism
from a symbiotic mixed culture E-1, consisting of Sphingomonas terrae and a Rhizobium sp.
Sphingopyxis terrae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-heptanol + acceptor
-
389911
Sphingopyxis terrae
n-heptanal + reduced acceptor
-
389911
Sphingopyxis terrae
?
1-hexanol + acceptor
-
389911
Sphingopyxis terrae
n-hexanal + reduced acceptor
-
389911
Sphingopyxis terrae
?
aromatic alcohol + acceptor
-
389911
Sphingopyxis terrae
aromatic aldehyde + reduced acceptor
-
389911
Sphingopyxis terrae
?
benzyl alcohol + acceptor
benzyl alcohol is the best substrate
389911
Sphingopyxis terrae
benzaldehyde + reduced acceptor
-
389911
Sphingopyxis terrae
?
PEG-1000 + acceptor
-
389911
Sphingopyxis terrae
?
-
-
-
?
PEG-300 + acceptor
-
389911
Sphingopyxis terrae
?
-
-
-
?
PEG-400 + acceptor
-
389911
Sphingopyxis terrae
?
-
-
-
?
PEG-6000 + acceptor
-
389911
Sphingopyxis terrae
?
-
-
-
?
polyethylene glycol + acceptor
-
389911
Sphingopyxis terrae
?
-
-
-
?
primary alcohol + acceptor
-
389911
Sphingopyxis terrae
aldehyde + reduced acceptor
-
389911
Sphingopyxis terrae
?
tetraethylene glycol + acceptor
-
389911
Sphingopyxis terrae
tetraethylene glycol aldehyde + reduced acceptor
-
389911
Sphingopyxis terrae
?
tetraethyleneglycol monoethyl ether
-
389911
Sphingopyxis terrae
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 58800, SDS-PAGE
Sphingopyxis terrae
Other publictions for EC 1.1.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725161
Liu
Catalytic mechanism of short e ...
Ensifer sp. AS08
Int. J. Mol. Sci.
14
1218-1231
2013
-
-
1
-
5
-
-
6
-
-
-
-
-
3
-
-
1
-
-
-
-
-
3
-
-
-
-
6
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
671546
Hu
Isolation of bacteria able to ...
Sphingopyxis macrogoltabida
Appl. Microbiol. Biotechnol.
73
1407-1413
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688864
Tani
Structure and conservation of ...
Sphingopyxis macrogoltabida 103, Sphingopyxis macrogoltabida, Sphingopyxis macrogoltabida 203, Sphingopyxis terrae
Microbiology
153
338-346
2007
-
2
2
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671461
Ohta
Analysis of amino acid residue ...
Sphingopyxis terrae
Appl. Environ. Microbiol.
72
4388-4396
2006
-
-
-
-
-
-
-
21
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389911
Sugimoto
The first step in polyethylene ...
Sphingopyxis terrae
J. Bacteriol.
183
6694-6698
2001
-
-
1
-
-
-
-
10
1
-
2
-
-
7
-
-
1
-
-
-
-
-
12
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
10
1
-
2
-
-
-
-
1
-
-
-
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389910
Yasuda
-
Polyethylene glycol dehydrogen ...
Rhodoblastus acidophilus, Rhodoblastus acidophilus M402
FEMS Microbiol. Lett.
138
23-28
1996
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389907
Yamanaka
-
Inducible or constitutive poly ...
Flavobacterium sp., Pseudomonas sp.
J. Ferment. Bioeng.
67
300-302
1989
4
-
-
-
-
-
2
-
2
3
-
-
-
2
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
2
-
-
-
4
-
-
2
-
-
-
-
2
-
-
2
3
-
-
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389908
Yamanaka
-
Purification and characterizat ...
Flavobacterium sp.
J. Ferment. Bioeng.
67
324-330
1989
1
-
-
-
-
-
14
4
1
-
2
-
-
1
-
-
1
-
-
-
2
-
36
1
1
1
2
-
2
1
1
5
-
-
-
1
-
-
5
-
-
-
-
14
-
4
1
-
2
-
-
-
-
1
-
-
2
-
36
1
1
1
2
-
2
1
1
-
-
-
-
-
-
-
389909
Kawai
Biodegradation of polyethylene ...
Flavobacterium sp., Pseudomonas sp.
Arch. Microbiol.
146
125-129
1986
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389904
Kawai
Identification of the prosthet ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
49
1071-1076
1985
4
-
-
-
-
-
8
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
10
-
-
-
4
-
-
10
-
-
-
-
8
-
4
2
-
-
-
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389905
Kawai
-
Identification of reaction pro ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
47
1669-1671
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389906
Kawai
Purification and characterizat ...
Flavobacterium sp., Pseudomonas sp.
Appl. Environ. Microbiol.
40
701-705
1980
-
-
-
-
-
-
22
4
-
4
4
-
-
3
-
-
2
-
-
-
2
-
28
2
2
2
4
-
2
2
6
2
-
-
-
-
-
-
2
-
-
-
-
22
-
4
-
4
4
-
-
-
-
2
-
-
2
-
28
2
2
2
4
-
2
2
6
-
-
-
-
-
-
-