BRENDA - Enzyme Database show
show all sequences of 1.1.99.20

Structure and conservation of a polyethylene glycol-degradative operon in sphingomonads

Tani, A.; Charoenpanich, J.; Mori, T.; Takeichi, M.; Kimbara, K.; Kawai, F.; Microbiology 153, 338-346 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
industry
microbiological degradation appears to be the only means to decompose this group of polymers
Sphingopyxis macrogoltabida
industry
microbiological degradation appears to be the only means to decompose this group of polymers
Sphingopyxis terrae
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli for sequencing, gene is located a large plasmid, PEG genes have evolved in a plasmid-mediated manner
Sphingopyxis macrogoltabida
expression in Escherichia coli for sequencing, gene is located a large plasmid, PEG genes have evolved in a plasmid-mediated manner
Sphingopyxis terrae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingopyxis macrogoltabida
-
-
-
Sphingopyxis macrogoltabida 103
-
-
-
Sphingopyxis macrogoltabida 203
-
-
-
Sphingopyxis terrae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
PEG-4000 + acceptor
-
688864
Sphingopyxis terrae
?
-
-
-
?
PEG-4000 + acceptor
enzyme is constitutively expressed
688864
Sphingopyxis macrogoltabida
?
-
-
-
?
PEG-4000 + acceptor
can not grow on PEG-6000
688864
Sphingopyxis macrogoltabida
?
-
-
-
?
PEG-4000 + acceptor
enzyme is constitutively expressed
688864
Sphingopyxis macrogoltabida 103
?
-
-
-
?
PEG-4000 + acceptor
can not grow on PEG-6000
688864
Sphingopyxis macrogoltabida 103
?
-
-
-
?
PEG-4000 + acceptor
enzyme is constitutively expressed
688864
Sphingopyxis macrogoltabida 203
?
-
-
-
?
PEG-4000 + acceptor
can not grow on PEG-6000
688864
Sphingopyxis macrogoltabida 203
?
-
-
-
?
PEG-6000 + acceptor
Sphingopyxis terrae can grow on PEG-20000
688864
Sphingopyxis terrae
?
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
industry
microbiological degradation appears to be the only means to decompose this group of polymers
Sphingopyxis macrogoltabida
industry
microbiological degradation appears to be the only means to decompose this group of polymers
Sphingopyxis terrae
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli for sequencing, gene is located a large plasmid, PEG genes have evolved in a plasmid-mediated manner
Sphingopyxis macrogoltabida
expression in Escherichia coli for sequencing, gene is located a large plasmid, PEG genes have evolved in a plasmid-mediated manner
Sphingopyxis terrae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
PEG-4000 + acceptor
-
688864
Sphingopyxis terrae
?
-
-
-
?
PEG-4000 + acceptor
enzyme is constitutively expressed
688864
Sphingopyxis macrogoltabida
?
-
-
-
?
PEG-4000 + acceptor
can not grow on PEG-6000
688864
Sphingopyxis macrogoltabida
?
-
-
-
?
PEG-4000 + acceptor
enzyme is constitutively expressed
688864
Sphingopyxis macrogoltabida 103
?
-
-
-
?
PEG-4000 + acceptor
can not grow on PEG-6000
688864
Sphingopyxis macrogoltabida 103
?
-
-
-
?
PEG-4000 + acceptor
enzyme is constitutively expressed
688864
Sphingopyxis macrogoltabida 203
?
-
-
-
?
PEG-4000 + acceptor
can not grow on PEG-6000
688864
Sphingopyxis macrogoltabida 203
?
-
-
-
?
PEG-6000 + acceptor
Sphingopyxis terrae can grow on PEG-20000
688864
Sphingopyxis terrae
?
-
-
-
?
Other publictions for EC 1.1.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725161
Liu
Catalytic mechanism of short e ...
Ensifer sp. AS08
Int. J. Mol. Sci.
14
1218-1231
2013
-
-
1
-
5
-
-
6
-
-
-
-
-
3
-
-
1
-
-
-
-
-
3
-
-
-
-
6
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
671546
Hu
Isolation of bacteria able to ...
Sphingopyxis macrogoltabida
Appl. Microbiol. Biotechnol.
73
1407-1413
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688864
Tani
Structure and conservation of ...
Sphingopyxis macrogoltabida 103, Sphingopyxis macrogoltabida, Sphingopyxis macrogoltabida 203, Sphingopyxis terrae
Microbiology
153
338-346
2007
-
2
2
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671461
Ohta
Analysis of amino acid residue ...
Sphingopyxis terrae
Appl. Environ. Microbiol.
72
4388-4396
2006
-
-
-
-
-
-
-
21
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389911
Sugimoto
The first step in polyethylene ...
Sphingopyxis terrae
J. Bacteriol.
183
6694-6698
2001
-
-
1
-
-
-
-
10
1
-
2
-
-
7
-
-
1
-
-
-
-
-
12
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
10
1
-
2
-
-
-
-
1
-
-
-
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389910
Yasuda
-
Polyethylene glycol dehydrogen ...
Rhodoblastus acidophilus, Rhodoblastus acidophilus M402
FEMS Microbiol. Lett.
138
23-28
1996
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389907
Yamanaka
-
Inducible or constitutive poly ...
Flavobacterium sp., Pseudomonas sp.
J. Ferment. Bioeng.
67
300-302
1989
4
-
-
-
-
-
2
-
2
3
-
-
-
2
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
2
-
-
-
4
-
-
2
-
-
-
-
2
-
-
2
3
-
-
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389908
Yamanaka
-
Purification and characterizat ...
Flavobacterium sp.
J. Ferment. Bioeng.
67
324-330
1989
1
-
-
-
-
-
14
4
1
-
2
-
-
1
-
-
1
-
-
-
2
-
36
1
1
1
2
-
2
1
1
5
-
-
-
1
-
-
5
-
-
-
-
14
-
4
1
-
2
-
-
-
-
1
-
-
2
-
36
1
1
1
2
-
2
1
1
-
-
-
-
-
-
-
389909
Kawai
Biodegradation of polyethylene ...
Flavobacterium sp., Pseudomonas sp.
Arch. Microbiol.
146
125-129
1986
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389904
Kawai
Identification of the prosthet ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
49
1071-1076
1985
4
-
-
-
-
-
8
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
10
-
-
-
4
-
-
10
-
-
-
-
8
-
4
2
-
-
-
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389905
Kawai
-
Identification of reaction pro ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
47
1669-1671
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389906
Kawai
Purification and characterizat ...
Flavobacterium sp., Pseudomonas sp.
Appl. Environ. Microbiol.
40
701-705
1980
-
-
-
-
-
-
22
4
-
4
4
-
-
3
-
-
2
-
-
-
2
-
28
2
2
2
4
-
2
2
6
2
-
-
-
-
-
-
2
-
-
-
-
22
-
4
-
4
4
-
-
-
-
2
-
-
2
-
28
2
2
2
4
-
2
2
6
-
-
-
-
-
-
-