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Literature summary for 1.1.99.22 extracted from

  • Ameyama, M.; Shinagawa, E.; Matsushita, K.; Adachi, O.
    Solubilization, purification and properties of membrane-bound glycerol dehydrogenase from Gluconobacter industrius (1985), Agric. Biol. Chem., 49, 1001-1010.
No PubMed abstract available

General Stability

General Stability Organism
amphoteric detergents, such as dimethyldodecylamineoxide, lauroylsarcosine, dodecylsulfobetaine, lauroylbetaine are effective solubilizers Gluconobacter oxydans
dimethyldodecylamineoxide, 0.5%, stabilizes Gluconobacter oxydans
glycerol stabilizes Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
glycerol membrane fraction Gluconobacter oxydans
19
-
meso-erythritol
-
Gluconobacter oxydans
22
-
D-arabitol
-
Gluconobacter oxydans
34
-
glycerol solubilized enzyme Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Gluconobacter oxydans 16020
-

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
maximum activity at the end of exponential growth phase, activity occurs in various Gluconobacter species, such as Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter suboxydans and Gluconobacter albidus
-
Gluconobacter oxydans IFO 3260
-
maximum activity at the end of exponential growth phase, activity occurs in various Gluconobacter species, such as Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter suboxydans and Gluconobacter albidus
-

Purification (Commentary)

Purification (Comment) Organism
-
Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
52
-
-
Gluconobacter oxydans

Storage Stability

Storage Stability Organism
-20┬░C, solubilized, stable for 6 months Gluconobacter oxydans
2-4┬░C, diluted enzyme solution, stable for 2 weeks Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adonitol + acceptor 42% of activity with glycerol Gluconobacter oxydans ?
-
?
adonitol + acceptor 42% of activity with glycerol Gluconobacter oxydans IFO 3260 ?
-
?
D-arabitol + acceptor 136% of activity with glycerol oxidation Gluconobacter oxydans ?
-
?
D-arabitol + acceptor 136% of activity with glycerol oxidation Gluconobacter oxydans IFO 3260 ?
-
?
D-mannitol + acceptor 26% of activity with glycerol Gluconobacter oxydans D-fructose + reduced acceptor
-
?
D-sorbitol + acceptor 45% of acticvity with glycerol Gluconobacter oxydans L-sorbose + reduced acceptor
-
?
D-sorbitol + acceptor 45% of acticvity with glycerol Gluconobacter oxydans IFO 3260 L-sorbose + reduced acceptor
-
?
galactitol + acceptor i.e. dulcitol, 29% of activity with glycerol Gluconobacter oxydans ?
-
?
glycerol + acceptor 2,6-dichlorophenolindophenol/phenazine methosulfate act as in vitro acceptor, broad specificity, no substrates are: xylitol, D-fructose, D-glucose, D-gluconate, 2-keto-D-gluconate, ethanol and acetaldehyde Gluconobacter oxydans glycerone + reduced acceptor i.e. dihydroxyacetone ?
glycerol + acceptor 2,6-dichlorophenolindophenol/phenazine methosulfate act as in vitro acceptor, broad specificity, no substrates are: xylitol, D-fructose, D-glucose, D-gluconate, 2-keto-D-gluconate, ethanol and acetaldehyde Gluconobacter oxydans IFO 3260 glycerone + reduced acceptor i.e. dihydroxyacetone ?
meso-erythritol + acceptor 111% of activity with glycerol Gluconobacter oxydans ?
-
?
meso-erythritol + acceptor 111% of activity with glycerol Gluconobacter oxydans IFO 3260 ?
-
?
propylene glycol + acceptor 54% of activity with glycerol Gluconobacter oxydans ?
-
?

Synonyms

Synonyms Comment Organism
More could be identical with non-purified EC 1.1.2.2 or D-arabitol dehydrogenase Gluconobacter oxydans

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
-
Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5 10 stable in the presence of glycerol and detergent Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
2,6-dichlorophenolindophenol acts in vitro as acceptor together with phenazine methosulfate Gluconobacter oxydans
additional information NAD+, NADP+ or molecular oxygen do not act as acceptors Gluconobacter oxydans
phenazine methosulfate acts in vitro as acceptor together with 2,6-dichlorophenolindophenol, linked to electron-transport chain of the organism Gluconobacter oxydans
pyrroloquinoline quinone quinoprotein Gluconobacter oxydans