BRENDA - Enzyme Database show
show all sequences of 1.1.99.26

Enzyme reactions involved in anaerobic cyclohexanol metabolism by a denitrifying Pseudomonas species

Dangel, W.; Tschech, A.; Fuchs, G.; Arch. Microbiol. 152, 273-279 (1989)
No PubMed abstract available

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
enzyme is induced by growth on cyclohexanone under anaerobic conditions
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.27
-
2-cyclohexenone hydratase and 3-hydroxycyclohexanone dehydrogenase activity in cell extracts
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-20C, cell extract stable for several days
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxycyclohexanone + acceptor
methylene blue or 2,6-dichlorophenolindophenol can function as electron acceptor in vitro
12556
Pseudomonas sp.
1,3-cyclohexanedione + reduced acceptor
-
12556
Pseudomonas sp.
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.27
-
2-cyclohexenone hydratase and 3-hydroxycyclohexanone dehydrogenase activity in cell extracts
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20C, cell extract stable for several days
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxycyclohexanone + acceptor
methylene blue or 2,6-dichlorophenolindophenol can function as electron acceptor in vitro
12556
Pseudomonas sp.
1,3-cyclohexanedione + reduced acceptor
-
12556
Pseudomonas sp.
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Pseudomonas sp.
Other publictions for EC 1.1.99.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
12556
Dangel
-
Enzyme reactions involved in a ...
Pseudomonas sp.
Arch. Microbiol.
152
273-279
1989
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