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Literature summary for 1.1.99.27 extracted from
- Purification and characterization of a novel FMN-dependent enzyme. Membrane-bound L-(+)-pantoyl lactone dehydrogenase from Nocardia asteroides (1992), Eur. J. Biochem., 204, 799-806.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Brij 35 | activates, maximal stimulation at 1.0% | Nocardia asteroides |  |
General Stability
| General Stability | Organism |
|---|---|
| Brij35 stabilizes | Nocardia asteroides |
| some stabilization by glycerol and ethylene glycol | Nocardia asteroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (R)-pantolactone | - |
Nocardia asteroides | |
| 2-Dehydropantonolactone | 20 mM | Nocardia asteroides | |
| 5,5'-dithiobis(2-nitrobenzoate) | 1 mM, 47% inhibition | Nocardia asteroides | |
| Atebrin | 1 mM, 32.6% inhibition | Nocardia asteroides | |
| Barbital | 1 mM, 54.5% inhibition | Nocardia asteroides | |
| CoCl2 | 1 mM, 24.4% inhibition | Nocardia asteroides | |
| Diphenylhydantoin | 1 mM, 22.1% inhibition | Nocardia asteroides | |
| iodoacetate | 1 mM, 17.4% inhibition | Nocardia asteroides | |
| MnCl2 | 1 mM, 86.2% inhibition | Nocardia asteroides | |
| NaAsO2 | 1 mM, 41% inhibition | Nocardia asteroides | |
| NaCN | 1 mM, 51.9% inhibition | Nocardia asteroides | |
| NaF | 1 mM, 27.3% inhibition | Nocardia asteroides | |
| NaN3 | 1 mM, 9.1% inhibition | Nocardia asteroides | |
| NEM | 1 mM, 33.8% inhibition | Nocardia asteroides | |
| NH2OH*HCl | 1 mM, 71.9% inhibition | Nocardia asteroides | |
| PCMB | 1 mM, 41.0% inhibition | Nocardia asteroides | |
| riboflavin | 1 mM, 74.9% inhibition | Nocardia asteroides | |
| Semicarbazide | 1 mM, 23.1% inhibition | Nocardia asteroides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.8 | - |
(R)-pantolactone | - |
Nocardia asteroides | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Nocardia asteroides | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol | Nocardia asteroides |
| 600000 | - |
above 600000 Da, HPLC gel filtration | Nocardia asteroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Nocardia asteroides | 1,2-propanediol induces the enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nocardia asteroides | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Nocardia asteroides |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Nocardia asteroides |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 7.4, 0.1 U/ml enzyme, 0.01 M Tris-HCl, 0.1 mM dithiothreitol, 0.5% Brij35, 2.9% loss of activity after 2 weeks | Nocardia asteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-Pantolactone + (Fe(CN)6)3- | - |
Nocardia asteroides | 2-Dehydropantonolactone + Fe(CN)64- | - |
? | |
| (R)-Pantolactone + 2,6-dichlorophenol-indophenol | - |
Nocardia asteroides | 2-Dehydropantonolactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| (R)-Pantolactone + indonitrotetrazolium | - |
Nocardia asteroides | 2-Dehydropantonolactone + reduced indonitrotetrazolium | - |
? | |
| (R)-Pantolactone + medola blue | - |
Nocardia asteroides | 2-Dehydropantonolactone + reduced medola blue | - |
? | |
| (R)-Pantolactone + nitrotetrazolium blue | - |
Nocardia asteroides | 2-Dehydropantonolactone + reduced nitrotetrazolium blue | - |
? | |
| (R)-Pantolactone + phenazine methosulfate | highly specific for (R)-pantolactone | Nocardia asteroides | 2-Dehydropantonolactone + reduced phenazine methosulfate | - |
? | |
| additional information | 1,2-propanediol induces the enzyme | Nocardia asteroides | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol | Nocardia asteroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Nocardia asteroides |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | about 20% of maximal activity at 25°C and 45°C | Nocardia asteroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 9.0-10.0, 30 min, stable | Nocardia asteroides |
| 35 | - |
pH 7.0, 10 min, stable below | Nocardia asteroides |
| 45 | - |
pH 7.0, 10 min, 43.1% loss of activity | Nocardia asteroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.5 | - |
Nocardia asteroides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | dependent on, noncovalently bound | Nocardia asteroides | |
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