Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Brij 35 | activates, maximal stimulation at 1.0% | Nocardia asteroides |
General Stability | Organism |
---|---|
Brij35 stabilizes | Nocardia asteroides |
some stabilization by glycerol and ethylene glycol | Nocardia asteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-pantolactone | - |
Nocardia asteroides | |
2-Dehydropantonolactone | 20 mM | Nocardia asteroides | |
5,5'-dithiobis(2-nitrobenzoate) | 1 mM, 47% inhibition | Nocardia asteroides | |
Atebrin | 1 mM, 32.6% inhibition | Nocardia asteroides | |
Barbital | 1 mM, 54.5% inhibition | Nocardia asteroides | |
CoCl2 | 1 mM, 24.4% inhibition | Nocardia asteroides | |
Diphenylhydantoin | 1 mM, 22.1% inhibition | Nocardia asteroides | |
iodoacetate | 1 mM, 17.4% inhibition | Nocardia asteroides | |
MnCl2 | 1 mM, 86.2% inhibition | Nocardia asteroides | |
NaAsO2 | 1 mM, 41% inhibition | Nocardia asteroides | |
NaCN | 1 mM, 51.9% inhibition | Nocardia asteroides | |
NaF | 1 mM, 27.3% inhibition | Nocardia asteroides | |
NaN3 | 1 mM, 9.1% inhibition | Nocardia asteroides | |
NEM | 1 mM, 33.8% inhibition | Nocardia asteroides | |
NH2OH*HCl | 1 mM, 71.9% inhibition | Nocardia asteroides | |
PCMB | 1 mM, 41.0% inhibition | Nocardia asteroides | |
riboflavin | 1 mM, 74.9% inhibition | Nocardia asteroides | |
Semicarbazide | 1 mM, 23.1% inhibition | Nocardia asteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.8 | - |
(R)-pantolactone | - |
Nocardia asteroides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Nocardia asteroides | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol | Nocardia asteroides |
600000 | - |
above 600000 Da, HPLC gel filtration | Nocardia asteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nocardia asteroides | 1,2-propanediol induces the enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia asteroides | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Nocardia asteroides |
Purification (Comment) | Organism |
---|---|
- |
Nocardia asteroides |
Storage Stability | Organism |
---|---|
4°C, pH 7.4, 0.1 U/ml enzyme, 0.01 M Tris-HCl, 0.1 mM dithiothreitol, 0.5% Brij35, 2.9% loss of activity after 2 weeks | Nocardia asteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-Pantolactone + (Fe(CN)6)3- | - |
Nocardia asteroides | 2-Dehydropantonolactone + Fe(CN)64- | - |
? | |
(R)-Pantolactone + 2,6-dichlorophenol-indophenol | - |
Nocardia asteroides | 2-Dehydropantonolactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
(R)-Pantolactone + indonitrotetrazolium | - |
Nocardia asteroides | 2-Dehydropantonolactone + reduced indonitrotetrazolium | - |
? | |
(R)-Pantolactone + medola blue | - |
Nocardia asteroides | 2-Dehydropantonolactone + reduced medola blue | - |
? | |
(R)-Pantolactone + nitrotetrazolium blue | - |
Nocardia asteroides | 2-Dehydropantonolactone + reduced nitrotetrazolium blue | - |
? | |
(R)-Pantolactone + phenazine methosulfate | highly specific for (R)-pantolactone | Nocardia asteroides | 2-Dehydropantonolactone + reduced phenazine methosulfate | - |
? | |
additional information | 1,2-propanediol induces the enzyme | Nocardia asteroides | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol | Nocardia asteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Nocardia asteroides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | about 20% of maximal activity at 25°C and 45°C | Nocardia asteroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 9.0-10.0, 30 min, stable | Nocardia asteroides |
35 | - |
pH 7.0, 10 min, stable below | Nocardia asteroides |
45 | - |
pH 7.0, 10 min, 43.1% loss of activity | Nocardia asteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | - |
Nocardia asteroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | dependent on, noncovalently bound | Nocardia asteroides |