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Literature summary for 1.1.99.29 extracted from

  • Volc, J.; Kubtov, E.; Wood, D.; Daniel, G.
    Pyranose 2-dehydrogenase, a novel sugar oxidoreductase from the basidiomycete fungus Agaricus bisporus (1997), Arch. Microbiol., 167, 119-125.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetate 50 mM: activates, 100 mM: 100% increase in activity at pH 4 Agaricus bisporus

General Stability

General Stability Organism
freezing in 50 mM sodium phosphate, 2.4 mg/ml, results in 90% loss of activity Agaricus bisporus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 10 mM, 100% inhibition Agaricus bisporus
CN- 10 mM, 83% inhibition Agaricus bisporus
Cu2+ 10 mM, 90% inhibition Agaricus bisporus
HgCl2 10 mM, 100% inhibition Agaricus bisporus
additional information not inhibited by Ca2+, Co2+, Fe2+, Fe3+, Mg2+, Mn2+, NH4+, Zn2+, F-, EDTA, NaN3, dithiothreitol, phenanthroline and H2O2, each at 10 mM Agaricus bisporus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular enzyme is actively secreted into the extracellular fluid Agaricus bisporus
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-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
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1 * 75000, SDS-PAGE Agaricus bisporus
79000
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gel filtration Agaricus bisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Agaricus bisporus oxidative carbohydrate metabolism, pyranose 2-dehydrogenase may play an important role in fungal lignocellulose decomposition by interconnecting ligninolysis with degradation of cell wall polysaccharide components ?
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?

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
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-
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Agaricus bisporus
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hybrid strain U3
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Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
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Agaricus bisporus

Purification (Commentary)

Purification (Comment) Organism
68.3fold Agaricus bisporus

Reaction

Reaction Comment Organism Reaction ID
a pyranoside + acceptor = a pyranosid-3-ulose (or a pyranosid-3,4-diulose) + reduced acceptor requires FAD, a number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as donor, 1,4-benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor, unlike EC 1.1.3.10, pyranose oxidase, this fungal enzyme does not interact with O2 and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars, D-glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3, the enzyme also acts on 1-4-alpha- and 1-4-beta-gluco-oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 1.1.3.10, sugars are oxidized in their pyranose but not in their furanose form Agaricus bisporus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium 17 days old Agaricus bisporus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.4
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pH 6.5, 25°C Agaricus bisporus

Storage Stability

Storage Stability Organism
4°C, purified enzyme, 100 mM sodium phosphate, pH 6, 7, 8 or 9, 100 days, stable Agaricus bisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a pyranoside + acceptor
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Agaricus bisporus a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
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?
aldopyranose + 1,4-benzoquinone more active as electron acceptor than 2,6-dichlorophenolindophenol or ferricyanide, less active than 3,5-di-tert-butyl-1,2-benzoquinone, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses Agaricus bisporus 2-aldoketose + hydroquinone
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?
aldopyranose + 2,6-dichlorophenolindophenol 65.9% of the activity with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses Agaricus bisporus 2-aldoketose + reduced 2,6-dichlorophenolindophenol
-
?
aldopyranose + 3,5-di-tert-butyl-1,2-benzoquinone 4.3fold higher activity than with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses Agaricus bisporus 2-aldoketose + reduced 3,5-di-tert-butyl-1,2-benzoquinone
-
?
aldopyranose + ferricyanide 10.4% of the activity with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses Agaricus bisporus 2-aldoketose + ferrocyanide
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?
aldopyranose + quinone C-2 specific sugar oxidoreductase, highly nonspecific, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses Agaricus bisporus 2-aldoketose + hydroquinone
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?
cellobiose + 1,4-benzoquinone 94% of the activity with D-glucose Agaricus bisporus ?
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?
cellotriose + 1,4-benzoquinone 56% of the activity with D-glucose Agaricus bisporus ?
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?
D-allose + 1,4-benzoquinone 30% of the activity with D-glucose Agaricus bisporus 2-dehydro-D-allose + hydroquinone
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?
D-arabino-2-hexosulose + 1,4-benzoquinone 59% of the activity with D-glucose Agaricus bisporus ?
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?
D-arabinose + 1,4-benzoquinone 18% of the activity with D-glucose Agaricus bisporus 2-dehydro-D-arabinose + hydroquinone
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?
D-galactose + 1,4-benzoquinone 88% of the activity with D-glucose Agaricus bisporus 2-dehydro-D-galactose + hydroquinone
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?
D-glucono-1,5-lactone + 1,4-benzoquinone 118% of the activity with D-glucose Agaricus bisporus ?
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?
D-glucose + 1,4-benzoquinone
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Agaricus bisporus 2-dehydro-D-glucose + hydroquinone D-arabino-2-hexosulose, 2-ketoglucose ?
D-ribose + 1,4-benzoquinone 34% of the activity with D-glucose Agaricus bisporus 2-dehydro-D-ribose + hydroquinone
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?
D-xylose + 1,4-benzoquinone 116% of the activity with D-glucose Agaricus bisporus 2-dehydro-D-xylose + hydroquinone
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?
L-arabinose + 1,4-benzoquinone 113% of the activity with D-glucose Agaricus bisporus 2-dehydro-L-arabinose + hydroquinone
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?
L-glucose + 1,4-benzoquinone 61% of the activity with D-glucose Agaricus bisporus 2-dehydro-L-glucose + hydroquinone
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?
L-sorbose + 1,4-benzoquinone 10% of the activity with D-glucose Agaricus bisporus 2-dehydro-L-sorbose + hydroquinone
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?
lactose + 1,4-benzoquinone 21% of the activity with D-glucose Agaricus bisporus ?
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?
maltose + 1,4-benzoquinone 93% of the activity with D-glucose Agaricus bisporus ?
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?
maltotriose + 1,4-benzoquinone 70% of the activity with D-glucose Agaricus bisporus ?
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?
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone 23% of the activity with D-glucose Agaricus bisporus methyl-alpha-3-dehydro-D-galactopyranoside + hydroquinone
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?
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone 117% of the activity with D-glucose Agaricus bisporus methyl-alpha-3-dehydro-D-glucopyranoside + hydroquinone
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?
methyl-beta-D-glucopyranoside + 1,4-benzoquinone 69% of the activity with D-glucose Agaricus bisporus methyl-beta-3-dehydro-D-glucopyranoside + hydroquinone
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?
additional information oxidative carbohydrate metabolism, pyranose 2-dehydrogenase may play an important role in fungal lignocellulose decomposition by interconnecting ligninolysis with degradation of cell wall polysaccharide components Agaricus bisporus ?
-
?
additional information enzyme is incapable of reducing O2 to H2O2, not: cytochrome c, NAD(P)+, nitroblue tetrazolium, low or no activity with D-altrose, D-fructose, D-lyxose, D-mannose, D-arabinitol, D-glucitol, D-mannitol or erythritol Agaricus bisporus ?
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?
pyranose + acceptor
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Agaricus bisporus 2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor
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?
sucrose + 1,4-benzoquinone 63% of the activity with D-glucose Agaricus bisporus ?
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?
trehalose + 1,4-benzoquinone 54% of the activity with D-glucose Agaricus bisporus ?
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?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Agaricus bisporus

Synonyms

Synonyms Comment Organism
dehydrogenase, pyranose 2/3-
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Agaricus bisporus
pyranose 2-dehydrogenase
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Agaricus bisporus
pyranose 2/3-dehydrogenase
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Agaricus bisporus
pyranose 3-dehydrogenase
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Agaricus bisporus
pyranose:quinone acceptor 2-oxidoreductase
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Agaricus bisporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
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assay at Agaricus bisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
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acetate buffer, two pH-optima Agaricus bisporus
9
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phosphate buffer, two pH-optima Agaricus bisporus

pH Range

pH Minimum pH Maximum Comment Organism
3 9.5 activity in the range is higher than 50% of the rate at the acidic optimum at pH 4 Agaricus bisporus

Cofactor

Cofactor Comment Organism Structure
flavin contains a flavin prosthetic group Agaricus bisporus

pI Value

Organism Comment pI Value Maximum pI Value
Agaricus bisporus analytical isoelectric focusing
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4.2