Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | at 0.1-1 M in 20 mM sodium phosphate buffer pH 7.0, not due to increased ionic strenth | Leucoagaricus meleagris |
Application | Comment | Organism |
---|---|---|
degradation | lignocellulose degradation | Leucoagaricus meleagris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha,alpha-dipyridyl | no activity | Leucoagaricus meleagris | |
CN- | no activity | Leucoagaricus meleagris | |
EDTA | no activity | Leucoagaricus meleagris | |
F- | no activity | Leucoagaricus meleagris | |
MoO42- | no activity | Leucoagaricus meleagris | |
NaN3 | no activity | Leucoagaricus meleagris | |
NH4+ | at 2 M in 20 mM sodium phosphate buffer pH 7.0 | Leucoagaricus meleagris | |
p-hydroxymercuribenzoate | no activity | Leucoagaricus meleagris | |
Phenanthroline | no activity | Leucoagaricus meleagris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
Fe3+ | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
0.18 | - |
methyl-1,4-benzoquinone | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
0.22 | - |
3,5-di-tert-butyl-1,4-benzoquinone | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
0.54 | - |
L-arabinose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
0.55 | - |
2-chloro-1,4-benzoquinone | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
0.82 | - |
D-glucose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
0.92 | - |
2,5-chloro-1,4-benzoquinone | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
1.05 | - |
D-galactose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.82 | - |
1,4-benzoquinone | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
1.93 | - |
D-xylose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
6.82 | - |
cellobiose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
8.61 | - |
D-maltose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
10.8 | - |
D-xylobiose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
59.7 | - |
D-ribose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
79.1 | - |
D-talose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
108 | - |
D-mannose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
134 | - |
lactose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
137 | - |
L-sorbose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
153 | - |
D-fructose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
156 | - |
maltotriose | Fe3+ as electron acceptor | Leucoagaricus meleagris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Leucoagaricus meleagris | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 37% inhibition of activity | Leucoagaricus meleagris | |
Al3+ | no activity | Leucoagaricus meleagris | |
Ca2+ | no activity | Leucoagaricus meleagris | |
Cu2+ | no activity | Leucoagaricus meleagris | |
Fe2+ | no activity | Leucoagaricus meleagris | |
Fe3+ | 39% inhibition of activity | Leucoagaricus meleagris | |
Hg2+ | 25% inhibition of activity | Leucoagaricus meleagris | |
Mg2+ | no activity | Leucoagaricus meleagris | |
Mn2+ | no activity | Leucoagaricus meleagris | |
Ni2+ | no activity | Leucoagaricus meleagris | |
Zn2+ | no activity | Leucoagaricus meleagris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
SDS-PAGE, PDHf, second band | Leucoagaricus meleagris |
66500 | - |
SDS-PAGE, gel filtration, MALDI MS | Leucoagaricus meleagris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucoagaricus meleagris | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content of 7% | Leucoagaricus meleagris |
Purification (Comment) | Organism |
---|---|
using DEAE-Sephacel anion exchange chromatography and FPLC on phenyl-superose HR 10/10, Mono Q HR 5/5 and superose 12 HR 30/10 chromatography | Leucoagaricus meleagris |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
stable for 3 months at 4°C, in sodium phosphate buffer pH 7.0 | Leucoagaricus meleagris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
D-fructose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
D-galactose + benzoquinone | - |
Leucoagaricus meleagris | 2-dehydro-D-galactose + hydroquinone | no 2,3-didehydro-d-galactose is detectable | ? | |
D-galactose + Fe3+ | - |
Leucoagaricus meleagris | 2-dehydro-D-galactose + Fe2+ + H+ | - |
? | |
D-glucose | pH 8.5 | Leucoagaricus meleagris | 3-dehydro-D-glucose + Fe2+ + H+ | - |
? | |
D-glucose + 2,5-chloro-1,4-benzoquinone | pH 5 | Leucoagaricus meleagris | ? | - |
? | |
D-glucose + 2,6-dichloroindophenol | pH 4 | Leucoagaricus meleagris | ? | - |
? | |
D-glucose + 2-chloro-1,4-benzoquinone | pH 5 | Leucoagaricus meleagris | ? | - |
? | |
D-glucose + 3,5-di-tert-butyl-1,4-benzoquinone | pH 6 | Leucoagaricus meleagris | ? | - |
? | |
D-glucose + benzoquinone | pH 3 | Leucoagaricus meleagris | 2,3-didehydro-D-glucose + hydroquinone | - |
? | |
D-glucose + methyl-1,4-benzoquinone | pH 8 | Leucoagaricus meleagris | ? | - |
? | |
D-maltose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
D-maltotriose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
D-mannose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
D-ribose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
D-talose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
D-xylobiose + ferricenium ion | - |
Leucoagaricus meleagris | ? | - |
r | |
D-xylose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
L-arabinose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
L-sorbose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
L-xylobiose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
lactose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
maltotriose + ferricenium ion | - |
Leucoagaricus meleagris | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration | Leucoagaricus meleagris |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Leucoagaricus meleagris |
pyranose dehydrogenase | - |
Leucoagaricus meleagris |
pyranose:acceptor oxidoreductase | - |
Leucoagaricus meleagris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
63 | - |
- |
Leucoagaricus meleagris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
50% activity loss after incubation at 50°C for 50 h | Leucoagaricus meleagris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
for glucose and 1,4-benzoquinone as substrates | Leucoagaricus meleagris |
9 | - |
Fe3+ as electron acceptor, with bisTris propane buffer | Leucoagaricus meleagris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 10 | depending on the electron acceptor and with d-glucose as electron donor, has 2 pH optima for some substrates | Leucoagaricus meleagris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
80% maximum stability at pH 7 | Leucoagaricus meleagris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 5 FAD covalently bound | Leucoagaricus meleagris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leucoagaricus meleagris | isoelectric focusing, 4 isoforms | - |
4.2 |
Leucoagaricus meleagris | isoelectric focusing, 4 isoforms | - |
4.25 |
Leucoagaricus meleagris | isoelectric focusing, 4 isoforms | - |
4.35 |
Leucoagaricus meleagris | isoelectric focusing, 4 isoforms | - |
4.45 |