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Literature summary for 1.1.99.3 extracted from
- Membrane-bound D-gluconate dehydrogenase of Serratia marcescens: purification and properties (1978), Agric. Biol. Chem., 42, 2355-2361.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oxamate | 1 mM, 91% loss of activity | Serratia marcescens |  |
| pyruvate | - |
Serratia marcescens | |
| Quinacrine hydrochloride | 1 mM, 10% loss of activity | Serratia marcescens | |
| Quinine hydrochloride | 1 mM, 42% loss of activity | Serratia marcescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
D-gluconate | - |
Serratia marcescens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Serratia marcescens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15500 | - |
1 * 15500 + 1 * 52500 + 1 * 68000, SDS-PAGE | Serratia marcescens |
| 52500 | - |
1 * 15500 + 1 * 52500 + 1 * 68000, SDS-PAGE | Serratia marcescens |
| 68000 | - |
1 * 15500 + 1 * 52500 + 1 * 68000, SDS-PAGE | Serratia marcescens |
| 136000 | - |
gel filtration | Serratia marcescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia marcescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Serratia marcescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 502 | - |
- |
Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-gluconate + 2,6-dichlorophenolindophenol | - |
Serratia marcescens | 2-oxogluconate + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 15500 + 1 * 52500 + 1 * 68000, SDS-PAGE | Serratia marcescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Serratia marcescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
10 min, stable below | Serratia marcescens |
| 50 | - |
10 min, about 50% loss of activity | Serratia marcescens |
| 60 | - |
10 min, about 90% loss of activity | Serratia marcescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Serratia marcescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | pH 4.0: about 40% of maximal activity, pH 6.0: about 60% of maximal activity | Serratia marcescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | 5°C, 16 h, stable | Serratia marcescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c1 | dehydrogenase protein is tightly bound to cytochrome c1 component | Serratia marcescens | |
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