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Literature summary for 1.1.99.31 extracted from

  • Lehoux, I.E.; Mitra, B.
    Role of arginine 277 in (S)-mandelate dehydrogenase from Pseudomonas putida in substrate binding and transition state stabilization (2000), Biochemistry, 39, 10055-10065.
    View publication on PubMed
Search for more literature for 1.1.99.31

Protein Variants

Protein Variants Comment Organism
R277G kcat for (S)-mandelate is 178fold lower than wild-type value, KM-value for (S)-mandelate is 160fold higher than wild-type value, KM-value for {(S)-3-phenyllactate is 1.6fold lower than wild-type value Pseudomonas putida
R277H kcat for (S)-mandelate is 1000fold lower than wild-type value, KM-value for (S)-mandelate is 608fold higher than wild-type value Pseudomonas putida
R277K kcat for (S)-mandelate is 3.7fold lower than wild-type value, KM-value for (S)-mandelate is 36.7fold higher than wild-type value Pseudomonas putida
R277L kcat for (S)-mandelate is 421fold lower fold than wild-type value, KM-value for (S)-mandelate is 392fold higher than wild-type value Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
 (S)-3-phenyllactate pH 7.5, 20°C, mutant enzyme R277G Pseudomonas putida
0.12
-
 (S)-Mandelate pH 7.5, 20°C, wild-type enzyme Pseudomonas putida
4.3
-
 (S)-3-phenyllactate pH 7.5, 20°C, wild-type enzyme Pseudomonas putida
4.4
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277K Pseudomonas putida
19.2
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277H Pseudomonas putida
47
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277G Pseudomonas putida
73
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277L Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzymes R277K, R277G, R277H and R277L Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-phenyllactate + acceptor
-
 Pseudomonas putida ?
-
 ?
(S)-mandelate + acceptor
-
 Pseudomonas putida 2-oxo-2-phenylacetate + reduced acceptor
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277L Pseudomonas putida
0.64
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277G Pseudomonas putida
1.52
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277H Pseudomonas putida
73
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277K Pseudomonas putida
201
-
 (S)-3-phenyllactate pH 7.5, 20°C, mutant enzyme R277G Pseudomonas putida
270
-
 (S)-Mandelate pH 7.5, 20°C, wild-type enzyme Pseudomonas putida
270
-
 (S)-3-phenyllactate pH 7.5, 20°C, wild-type enzyme Pseudomonas putida