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Literature summary for 1.1.99.32 extracted from

  • Han, X.; Xiong, X.; Jiang, D.; Chen, S.; Huang, E.; Zhang, W.; Liu, X.
    Crystal structure of L-sorbose dehydrogenase, a pyrroloquinoline quinone-dependent enzyme with homodimeric assembly, from Ketogulonicigenium vulgare (2014), Biotechnol. Lett., 36, 1001-1008.
    View publication on PubMed
Search for more literature for 1.1.99.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli codon plus cells Ketogulonicigenium vulgare

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 1.7 M ammonium sulfate, 5 mM pyrroloquinoline quinone, 5 mM CaCl2, and 0.1 M pH 7.4 HEPES Ketogulonicigenium vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Ketogulonicigenium vulgare 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Ketogulonicigenium vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
117200
-
 calculated from amino acid sequence Ketogulonicigenium vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-sorbose + cytochrome c551 Ketogulonicigenium vulgare
-
 L-sorbosone + reduced cytochrome c551
-
 ?
L-sorbose + cytochrome c551 Ketogulonicigenium vulgare Y25
-
 L-sorbosone + reduced cytochrome c551
-
 ?

Organism

Organism UniProt Comment Textmining
Ketogulonicigenium vulgare
-
-
-
Ketogulonicigenium vulgare Y25
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, HiTrap Q column chromatography, and Superdex 200 gel filtration Ketogulonicigenium vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-sorbose + cytochrome c551
-
 Ketogulonicigenium vulgare L-sorbosone + reduced cytochrome c551
-
 ?
L-sorbose + cytochrome c551
-
 Ketogulonicigenium vulgare Y25 L-sorbosone + reduced cytochrome c551
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Ketogulonicigenium vulgare

Synonyms

Synonyms Comment Organism
L-sorbose dehydrogenase
-
 Ketogulonicigenium vulgare
SDH
-
 Ketogulonicigenium vulgare

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
 Ketogulonicigenium vulgare