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Literature summary for 1.1.99.35 extracted from

  • Olsthoorn, A.J.; Duine, J.A.
    On the mechanism and specificity of soluble, quinoprotein glucose dehydrogenase in the oxidation of aldose sugars (1998), Biochemistry, 37, 13854-13861.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
cellobiose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-fucose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-fucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-lyxose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-lyxono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-ribose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
L-arabinose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus L-arabinono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
melibiose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
?
additional information kinetic mechanism of sGDH consists of (a) step(s) in which a fluorescing intermediate is formed, and a subsequent, irreversible step, determining the overall rate of the reductive half-reaction. The pH optimum determined for glucose oxidation appears to be 7.0, implying that reoxidation of sGDHred is rate-limiting with those electron acceptors displaying a different value under steady-state conditions. Acinetobacter calcoaceticus ?
-
?

Synonyms

Synonyms Comment Organism
sGDH
-
Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
pH optimum determined for glucose oxidation appears to be 7.0, implying that reoxidation of sGDHred is rate-limiting with those electron acceptors displaying a different value under steady-state conditions Acinetobacter calcoaceticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.24
-
D-Lyxose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
11
-
D-ribose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
11
-
melibiose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
45
-
D-xylose steady-state kinetics, 20┬░C Acinetobacter calcoaceticus
46
-
D-xylose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
50
-
D-mannose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
150
-
D-galactose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
240
-
D-galactose steady-state kinetics, 20┬░C Acinetobacter calcoaceticus
440
-
D-fucose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
790
-
lactose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
800
-
maltose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
960
-
lactose steady-state kinetics, 20┬░C Acinetobacter calcoaceticus
1080
-
cellobiose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
1450
-
L-arabinose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
1470
-
D-allose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
1700
-
D-glucose stopped-flow measurement of reductive half-reaction, 15┬░C Acinetobacter calcoaceticus
2100
-
D-glucose steady-state kinetics, 20┬░C Acinetobacter calcoaceticus