Protein Variants | Comment | Organism |
---|---|---|
D275E | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 11%, D-allose 44%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 7%, D-lactose 52%, D-maltose 44%, respectively | Acinetobacter calcoaceticus |
D276E | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 13%, D-mannose 19%, D-allose 54%, 3-O-methyl-D-glucose 57%, D-galactose 26%, D-xylose 20%, D-lactose 75%, D-maltose 66%, respectively | Acinetobacter calcoaceticus |
E277A | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 11%, D-mannose 33%, D-allose 142%, 3-O-methyl-D-glucose 91%, D-galactose 46%, D-xylose 23%, D-lactose 83%, D-maltose 39%, respectively | Acinetobacter calcoaceticus |
E277D | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 9%, D-mannose 19%, D-allose 68%, 3-O-methyl-D-glucose 58%, D-galactose 21%, D-xylose 15%, D-lactose 87%, D-maltose 73%, respectively | Acinetobacter calcoaceticus |
E277G | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 15%, D-mannose 38%, D-allose 189%, 3-O-methyl-D-glucose 113%, D-galactose 59%, D-xylose 28%, D-lactose 100%, D-maltose 52%, respectively | Acinetobacter calcoaceticus |
E277H | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 11%, D-allose 67%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 9%, D-lactose 57%, D-maltose 39%, respectively | Acinetobacter calcoaceticus |
E277K | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 7%, D-mannose 18%, D-allose 116%, 3-O-methyl-D-glucose 84%, D-galactose 28%, D-xylose 18%, D-lactose 79%, D-maltose 74%, respectively | Acinetobacter calcoaceticus |
E277N | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 148%, 3-O-methyl-D-glucose 91%, D-galactose 43%, D-xylose 18%, D-lactose 86%, D-maltose 59%, respectively | Acinetobacter calcoaceticus |
E277Q | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 10%, D-mannose 31%, D-allose 134%, 3-O-methyl-D-glucose 80%, D-galactose 35%, D-xylose 25%, D-lactose 68%, D-maltose 54%, respectively | Acinetobacter calcoaceticus |
E277V | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 150%, 3-O-methyl-D-glucose 101%, D-galactose 25%, D-xylose 19%, D-lactose 114%, D-maltose 65%, respectively | Acinetobacter calcoaceticus |
I278F | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 4%, D-mannose 14%, D-allose 54%, 3-O-methyl-D-glucose 47%, D-galactose 15%, D-xylose 12%, D-lactose 64%, D-maltose 49%, respectively | Acinetobacter calcoaceticus |
N279H | substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 6%, D-allose 49%, 3-O-methyl-D-glucose 50%, D-galactose 13%, D-xylose 8%, D-lactose 64%, D-maltose 61%, respectively | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
D-galactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
6.8 | - |
D-galactose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
7.5 | - |
D-xylose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
8.8 | - |
D-glucose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
14.3 | - |
D-xylose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
14.3 | - |
D-lactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
14.3 | - |
D-maltose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
21 | - |
D-allose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
22 | - |
D-mannose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
22 | - |
D-mannose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
26.8 | - |
D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
27 | - |
3-O-methyl-D-glucose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
29 | - |
D-allose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
30.9 | - |
D-maltose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
34 | - |
D-lactose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
46 | - |
3-O-methyl-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
88 | - |
2-deoxy-D-glucose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
90 | - |
2-deoxy-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol | 4% of the activity with D-glucose | Acinetobacter calcoaceticus | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol | 81% of the activity with D-glucose | Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-allose + oxidized 2,6-dichlorophenolindophenol | 47% of the activity with D-glucose | Acinetobacter calcoaceticus | D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-galactose + oxidized 2,6-dichlorophenolindophenol | 11% of the activity with D-glucose | Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-mannose + oxidized 2,6-dichlorophenolindophenol | 13% of the activity with D-glucose | Acinetobacter calcoaceticus | D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + oxidized 2,6-dichlorophenolindophenol | 7% of the activity with D-glucose | Acinetobacter calcoaceticus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + oxidized 2,6-dichlorophenolindophenol | 61% of the activity with D-glucose | Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + oxidized 2,6-dichlorophenolindophenol | 61% of the activity with D-glucose | Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenolindophenol | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
121 | - |
D-galactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
201 | - |
D-lactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
267 | - |
D-mannose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
331 | - |
2-deoxy-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
630 | - |
D-galactose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
669 | - |
D-xylose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
678 | - |
D-lactose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
785 | - |
D-maltose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
861 | - |
D-mannose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
1015 | - |
D-maltose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
1063 | - |
2-deoxy-D-glucose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
1443 | - |
D-allose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1448 | - |
3-O-methyl-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
1795 | - |
D-xylose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
3071 | - |
D-glucose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
3198 | - |
3-O-methyl-D-glucose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
3436 | - |
D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
4563 | - |
D-allose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
2-deoxy-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
7 | - |
D-lactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
12 | - |
2-deoxy-D-glucose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
12 | - |
D-mannose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
18 | - |
D-galactose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
20 | - |
D-lactose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
25 | - |
D-maltose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
31 | - |
3-O-methyl-D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
39 | - |
D-mannose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
47 | - |
D-xylose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
50 | - |
D-allose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
71 | - |
D-maltose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
78 | - |
D-galactose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
118 | - |
3-O-methyl-D-glucose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
128 | - |
D-glucose | wild-type, pH 7.0 | Acinetobacter calcoaceticus | |
239 | - |
D-xylose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
287 | - |
D-allose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus | |
349 | - |
D-glucose | mutant E277K, pH 7.0 | Acinetobacter calcoaceticus |