BRENDA - Enzyme Database show
show all sequences of 1.1.99.35

Construction and characterization of mutant water-soluble PQQ glucose dehydrogenases with altered K(m) values--site-directed mutagenesis studies on the putative active site

Igarashi, S.; Ohtera, T.; Yoshida, H.; Witarto, A.B.; Sode, K.; Biochem. Biophys. Res. Commun. 264, 820-824 (1999)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D275E
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 11%, D-allose 44%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 7%, D-lactose 52%, D-maltose 44%, respectively
Acinetobacter calcoaceticus
D276E
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 13%, D-mannose 19%, D-allose 54%, 3-O-methyl-D-glucose 57%, D-galactose 26%, D-xylose 20%, D-lactose 75%, D-maltose 66%, respectively
Acinetobacter calcoaceticus
E277A
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 11%, D-mannose 33%, D-allose 142%, 3-O-methyl-D-glucose 91%, D-galactose 46%, D-xylose 23%, D-lactose 83%, D-maltose 39%, respectively
Acinetobacter calcoaceticus
E277D
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 9%, D-mannose 19%, D-allose 68%, 3-O-methyl-D-glucose 58%, D-galactose 21%, D-xylose 15%, D-lactose 87%, D-maltose 73%, respectively
Acinetobacter calcoaceticus
E277G
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 15%, D-mannose 38%, D-allose 189%, 3-O-methyl-D-glucose 113%, D-galactose 59%, D-xylose 28%, D-lactose 100%, D-maltose 52%, respectively
Acinetobacter calcoaceticus
E277H
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 11%, D-allose 67%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 9%, D-lactose 57%, D-maltose 39%, respectively
Acinetobacter calcoaceticus
E277K
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 7%, D-mannose 18%, D-allose 116%, 3-O-methyl-D-glucose 84%, D-galactose 28%, D-xylose 18%, D-lactose 79%, D-maltose 74%, respectively
Acinetobacter calcoaceticus
E277N
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 148%, 3-O-methyl-D-glucose 91%, D-galactose 43%, D-xylose 18%, D-lactose 86%, D-maltose 59%, respectively
Acinetobacter calcoaceticus
E277Q
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 10%, D-mannose 31%, D-allose 134%, 3-O-methyl-D-glucose 80%, D-galactose 35%, D-xylose 25%, D-lactose 68%, D-maltose 54%, respectively
Acinetobacter calcoaceticus
E277V
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 150%, 3-O-methyl-D-glucose 101%, D-galactose 25%, D-xylose 19%, D-lactose 114%, D-maltose 65%, respectively
Acinetobacter calcoaceticus
I278F
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 4%, D-mannose 14%, D-allose 54%, 3-O-methyl-D-glucose 47%, D-galactose 15%, D-xylose 12%, D-lactose 64%, D-maltose 49%, respectively
Acinetobacter calcoaceticus
N279H
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 6%, D-allose 49%, 3-O-methyl-D-glucose 50%, D-galactose 13%, D-xylose 8%, D-lactose 64%, D-maltose 61%, respectively
Acinetobacter calcoaceticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.8
-
D-galactose
mutant E277K, pH 7.0; wild-type, pH 7.0
Acinetobacter calcoaceticus
7.5
-
D-xylose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
8.8
-
D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
14.3
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
14.3
-
D-maltose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
14.3
-
D-xylose
wild-type, pH 7.0
Acinetobacter calcoaceticus
21
-
D-Allose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
22
-
D-mannose
mutant E277K, pH 7.0; wild-type, pH 7.0
Acinetobacter calcoaceticus
26.8
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
27
-
3-O-methyl-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
29
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
30.9
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
34
-
D-lactose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
46
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
88
-
2-deoxy-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
90
-
2-deoxy-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter calcoaceticus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
4% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
81% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
47% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
11% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
639216
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
13% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
7% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
121
-
D-galactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
201
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
267
-
D-mannose
wild-type, pH 7.0
Acinetobacter calcoaceticus
331
-
2-deoxy-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
630
-
D-galactose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
669
-
D-xylose
wild-type, pH 7.0
Acinetobacter calcoaceticus
678
-
D-lactose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
785
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
861
-
D-mannose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
1015
-
D-maltose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
1063
-
2-deoxy-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
1443
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
1448
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
1795
-
D-xylose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
3071
-
D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
3198
-
3-O-methyl-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
3436
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
4563
-
D-Allose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D275E
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 11%, D-allose 44%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 7%, D-lactose 52%, D-maltose 44%, respectively
Acinetobacter calcoaceticus
D276E
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 13%, D-mannose 19%, D-allose 54%, 3-O-methyl-D-glucose 57%, D-galactose 26%, D-xylose 20%, D-lactose 75%, D-maltose 66%, respectively
Acinetobacter calcoaceticus
E277A
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 11%, D-mannose 33%, D-allose 142%, 3-O-methyl-D-glucose 91%, D-galactose 46%, D-xylose 23%, D-lactose 83%, D-maltose 39%, respectively
Acinetobacter calcoaceticus
E277D
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 9%, D-mannose 19%, D-allose 68%, 3-O-methyl-D-glucose 58%, D-galactose 21%, D-xylose 15%, D-lactose 87%, D-maltose 73%, respectively
Acinetobacter calcoaceticus
E277G
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 15%, D-mannose 38%, D-allose 189%, 3-O-methyl-D-glucose 113%, D-galactose 59%, D-xylose 28%, D-lactose 100%, D-maltose 52%, respectively
Acinetobacter calcoaceticus
E277H
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 11%, D-allose 67%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 9%, D-lactose 57%, D-maltose 39%, respectively
Acinetobacter calcoaceticus
E277K
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 7%, D-mannose 18%, D-allose 116%, 3-O-methyl-D-glucose 84%, D-galactose 28%, D-xylose 18%, D-lactose 79%, D-maltose 74%, respectively
Acinetobacter calcoaceticus
E277N
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 148%, 3-O-methyl-D-glucose 91%, D-galactose 43%, D-xylose 18%, D-lactose 86%, D-maltose 59%, respectively
Acinetobacter calcoaceticus
E277Q
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 10%, D-mannose 31%, D-allose 134%, 3-O-methyl-D-glucose 80%, D-galactose 35%, D-xylose 25%, D-lactose 68%, D-maltose 54%, respectively
Acinetobacter calcoaceticus
E277V
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 150%, 3-O-methyl-D-glucose 101%, D-galactose 25%, D-xylose 19%, D-lactose 114%, D-maltose 65%, respectively
Acinetobacter calcoaceticus
I278F
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 4%, D-mannose 14%, D-allose 54%, 3-O-methyl-D-glucose 47%, D-galactose 15%, D-xylose 12%, D-lactose 64%, D-maltose 49%, respectively
Acinetobacter calcoaceticus
N279H
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 6%, D-allose 49%, 3-O-methyl-D-glucose 50%, D-galactose 13%, D-xylose 8%, D-lactose 64%, D-maltose 61%, respectively
Acinetobacter calcoaceticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.8
-
D-galactose
mutant E277K, pH 7.0; wild-type, pH 7.0
Acinetobacter calcoaceticus
7.5
-
D-xylose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
8.8
-
D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
14.3
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
14.3
-
D-maltose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
14.3
-
D-xylose
wild-type, pH 7.0
Acinetobacter calcoaceticus
21
-
D-Allose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
22
-
D-mannose
mutant E277K, pH 7.0; wild-type, pH 7.0
Acinetobacter calcoaceticus
26.8
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
27
-
3-O-methyl-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
29
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
30.9
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
34
-
D-lactose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
46
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
88
-
2-deoxy-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
90
-
2-deoxy-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
4% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
81% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
47% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
11% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
639216
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
13% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
7% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
639216
Acinetobacter calcoaceticus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
121
-
D-galactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
201
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
267
-
D-mannose
wild-type, pH 7.0
Acinetobacter calcoaceticus
331
-
2-deoxy-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
630
-
D-galactose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
669
-
D-xylose
wild-type, pH 7.0
Acinetobacter calcoaceticus
678
-
D-lactose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
785
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
861
-
D-mannose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
1015
-
D-maltose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
1063
-
2-deoxy-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
1443
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
1448
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
1795
-
D-xylose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
3071
-
D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
3198
-
3-O-methyl-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
3436
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
4563
-
D-Allose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4
-
2-deoxy-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
7
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
12
-
2-deoxy-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
12
-
D-mannose
wild-type, pH 7.0
Acinetobacter calcoaceticus
18
-
D-galactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
20
-
D-lactose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
25
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
31
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
39
-
D-mannose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
47
-
D-xylose
wild-type, pH 7.0
Acinetobacter calcoaceticus
50
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
71
-
D-maltose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
78
-
D-galactose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
118
-
3-O-methyl-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
128
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
239
-
D-xylose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
287
-
D-Allose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
349
-
D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4
-
2-deoxy-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
7
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
12
-
2-deoxy-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
12
-
D-mannose
wild-type, pH 7.0
Acinetobacter calcoaceticus
18
-
D-galactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
20
-
D-lactose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
25
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
31
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
39
-
D-mannose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
47
-
D-xylose
wild-type, pH 7.0
Acinetobacter calcoaceticus
50
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
71
-
D-maltose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
78
-
D-galactose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
118
-
3-O-methyl-D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
128
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
239
-
D-xylose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
287
-
D-Allose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
349
-
D-glucose
mutant E277K, pH 7.0
Acinetobacter calcoaceticus
Other publictions for EC 1.1.99.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739914
An
Regulation of pyrroloquinoline ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
82
4955-4964
2016
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1
1
1
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42
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4
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4
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1
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1
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-
739848
Flexer
Wired pyrroloquinoline quinone ...
Acinetobacter calcoaceticus
Anal. Chem.
86
2465-2473
2014
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-
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2
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1
-
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-
739872
Ben Farhat
Coexpression of the pyrroloqui ...
Serratia marcescens, Serratia marcescens CTM 50650
Appl. Biochem. Biotechnol.
170
1738-1750
2013
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1
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1
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4
-
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-
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-
-
-
739873
Stredansky
Monitoring of PQQ-dependent gl ...
Acinetobacter calcoaceticus
Appl. Biochem. Biotechnol.
171
1032-1041
2013
-
-
-
-
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1
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13
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1
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1
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-
-
13
-
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-
-
-
-
-
-
-
-
-
-
12
12
724836
Schubart
-
A pyrroloquinolinequinone-depe ...
Acinetobacter calcoaceticus
Electrochim. Acta
82
224-232
2012
-
1
-
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1
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1
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1
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1
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1
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1
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1
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1
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-
1
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-
-
724852
Yu
-
High-level production of solub ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus L.M.D. 79.41
Eng. Life Sci.
12
574-582
2012
-
-
1
-
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1
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1
10
-
3
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-
1
-
-
1
2
-
10
1
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-
1
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1
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-
1
1
-
-
-
-
-
-
-
1
-
1
10
-
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1
-
1
2
-
10
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
723943
Flexer
Efficient direct electron tran ...
Acinetobacter calcoaceticus
Anal. Chem.
83
5721-5727
2011
-
-
-
-
1
1
-
-
1
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-
1
-
2
-
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-
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2
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1
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1
-
1
1
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1
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-
1
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724172
Durand
Designing a highly active solu ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
402
750-754
2010
-
-
1
-
1
-
-
2
-
-
1
1
-
1
-
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-
-
-
-
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-
1
1
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-
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1
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1
1
-
1
-
-
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-
2
-
-
1
1
-
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-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655127
Tanaka
Increasing stability of water- ...
Acinetobacter calcoaceticus
BMC Biochem.
6
1
2005
-
-
-
-
3
-
-
2
-
-
-
-
-
1
-
-
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-
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1
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5
-
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-
3
-
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2
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-
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
655929
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
J. Biochem. Biophys. Methods
61
331-338
2004
-
-
1
-
1
-
-
11
-
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-
1
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-
-
-
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6
1
-
-
-
-
-
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-
1
-
-
1
-
-
-
-
11
-
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-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654863
Oubrie
Structure and mechanism of sol ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1647
143-151
2003
-
-
-
1
-
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1
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1
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1
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1
-
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-
-
-
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-
-
-
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-
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-
-
654398
Sode
-
Construction of engineered wat ...
Acinetobacter calcoaceticus
Biocatal. Biotransform.
20
405-412
2002
-
-
-
-
5
-
-
18
-
-
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1
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-
6
-
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18
-
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5
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18
-
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-
6
-
-
-
-
18
-
-
-
-
-
-
-
-
18
18
655363
Yoshida
-
Secretion of water soluble pyr ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
30
312-318
2002
-
1
1
-
1
-
-
4
-
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1
-
1
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2
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1
1
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1
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4
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1
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-
2
-
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-
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-
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-
-
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-
639218
Sode
Increasing the thermal stabili ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
26
491-496
2000
-
-
-
-
7
-
-
2
-
-
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1
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-
2
-
9
-
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1
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7
-
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2
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-
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-
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-
2
-
9
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
702202
Dewanti
Ca2+-assisted, direct hydride ...
Acinetobacter calcoaceticus
Biochemistry
39
9384-9392
2000
-
-
-
-
-
-
-
-
-
-
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2
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702811
Kojima
-
The production of soluble pyrr ...
Acinetobacter calcoaceticus
Biotechnol. Lett.
22
1343-1347
2000
-
1
1
-
-
-
-
-
-
-
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1
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-
-
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1
1
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-
-
-
-
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-
-
-
-
-
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-
-
-
-
-
-
-
-
639204
Oubrie
The 1.7 A crystal structure of ...
Acinetobacter calcoaceticus
J. Mol. Biol.
289
319-333
1999
-
-
-
1
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1
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2
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1
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1
1
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1
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-
-
-
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-
-
-
-
639216
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
264
820-824
1999
-
-
-
-
12
-
-
16
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
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18
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
18
-
-
-
-
-
-
-
-
18
18
639220
Oubrie
Active-site structure of the s ...
Acinetobacter calcoaceticus
Proc. Natl. Acad. Sci. USA
96
11787-11791
1999
-
-
-
1
-
-
1
-
-
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-
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2
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1
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-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639209
Olsthoorn
On the mechanism and specifici ...
Acinetobacter calcoaceticus
Biochemistry
37
13854-13861
1998
-
-
-
-
-
-
-
-
-
-
-
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1
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16
-
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-
1
-
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-
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-
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-
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-
-
16
-
-
-
-
-
1
-
-
-
-
-
-
-
17
17
703532
Olsthoorn
Negative cooperativity in the ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
255
255-261
1998
-
-
-
-
-
-
2
1
-
2
-
-
-
2
-
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-
1
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-
-
-
4
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
3
1
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703530
Olsthoorn
Ca2+ and its substitutes have ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
247
659-665
1997
-
-
-
-
-
-
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-
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1
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1
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1
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-
-
-
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-
-
-
-
-
-
702726
Matsushita
-
Soluble and membrane-bound qui ...
Acinetobacter calcoaceticus
Biosci. Biotechnol. Biochem.
59
1548-1555
1995
-
-
-
-
-
-
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5
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1
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1
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1
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5
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705107
Schlunegger
Crystallization and preliminar ...
Acinetobacter calcoaceticus
J. Mol. Biol.
233
784-786
1993
-
-
-
1
-
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1
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1
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