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Literature summary for 1.1.99.35 extracted from

  • Igarashi, S.; Ohtera, T.; Yoshida, H.; Witarto, A.B.; Sode, K.
    Construction and characterization of mutant water-soluble PQQ glucose dehydrogenases with altered K(m) values--site-directed mutagenesis studies on the putative active site (1999), Biochem. Biophys. Res. Commun., 264, 820-824.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D275E substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 11%, D-allose 44%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 7%, D-lactose 52%, D-maltose 44%, respectively Acinetobacter calcoaceticus
D276E substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 13%, D-mannose 19%, D-allose 54%, 3-O-methyl-D-glucose 57%, D-galactose 26%, D-xylose 20%, D-lactose 75%, D-maltose 66%, respectively Acinetobacter calcoaceticus
E277A substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 11%, D-mannose 33%, D-allose 142%, 3-O-methyl-D-glucose 91%, D-galactose 46%, D-xylose 23%, D-lactose 83%, D-maltose 39%, respectively Acinetobacter calcoaceticus
E277D substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 9%, D-mannose 19%, D-allose 68%, 3-O-methyl-D-glucose 58%, D-galactose 21%, D-xylose 15%, D-lactose 87%, D-maltose 73%, respectively Acinetobacter calcoaceticus
E277G substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 15%, D-mannose 38%, D-allose 189%, 3-O-methyl-D-glucose 113%, D-galactose 59%, D-xylose 28%, D-lactose 100%, D-maltose 52%, respectively Acinetobacter calcoaceticus
E277H substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 11%, D-allose 67%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 9%, D-lactose 57%, D-maltose 39%, respectively Acinetobacter calcoaceticus
E277K substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 7%, D-mannose 18%, D-allose 116%, 3-O-methyl-D-glucose 84%, D-galactose 28%, D-xylose 18%, D-lactose 79%, D-maltose 74%, respectively Acinetobacter calcoaceticus
E277N substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 148%, 3-O-methyl-D-glucose 91%, D-galactose 43%, D-xylose 18%, D-lactose 86%, D-maltose 59%, respectively Acinetobacter calcoaceticus
E277Q substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 10%, D-mannose 31%, D-allose 134%, 3-O-methyl-D-glucose 80%, D-galactose 35%, D-xylose 25%, D-lactose 68%, D-maltose 54%, respectively Acinetobacter calcoaceticus
E277V substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 150%, 3-O-methyl-D-glucose 101%, D-galactose 25%, D-xylose 19%, D-lactose 114%, D-maltose 65%, respectively Acinetobacter calcoaceticus
I278F substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 4%, D-mannose 14%, D-allose 54%, 3-O-methyl-D-glucose 47%, D-galactose 15%, D-xylose 12%, D-lactose 64%, D-maltose 49%, respectively Acinetobacter calcoaceticus
N279H substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 6%, D-allose 49%, 3-O-methyl-D-glucose 50%, D-galactose 13%, D-xylose 8%, D-lactose 64%, D-maltose 61%, respectively Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.8
-
D-galactose wild-type, pH 7.0 Acinetobacter calcoaceticus
6.8
-
D-galactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
7.5
-
D-xylose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
8.8
-
D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
14.3
-
D-xylose wild-type, pH 7.0 Acinetobacter calcoaceticus
14.3
-
D-lactose wild-type, pH 7.0 Acinetobacter calcoaceticus
14.3
-
D-maltose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
21
-
D-allose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
22
-
D-mannose wild-type, pH 7.0 Acinetobacter calcoaceticus
22
-
D-mannose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
26.8
-
D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
27
-
3-O-methyl-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
29
-
D-allose wild-type, pH 7.0 Acinetobacter calcoaceticus
30.9
-
D-maltose wild-type, pH 7.0 Acinetobacter calcoaceticus
34
-
D-lactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
46
-
3-O-methyl-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
88
-
2-deoxy-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
90
-
2-deoxy-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol 4% of the activity with D-glucose Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol 81% of the activity with D-glucose Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol 47% of the activity with D-glucose Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol 11% of the activity with D-glucose Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol 13% of the activity with D-glucose Acinetobacter calcoaceticus D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol 7% of the activity with D-glucose Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
lactose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
121
-
D-galactose wild-type, pH 7.0 Acinetobacter calcoaceticus
201
-
D-lactose wild-type, pH 7.0 Acinetobacter calcoaceticus
267
-
D-mannose wild-type, pH 7.0 Acinetobacter calcoaceticus
331
-
2-deoxy-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
630
-
D-galactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
669
-
D-xylose wild-type, pH 7.0 Acinetobacter calcoaceticus
678
-
D-lactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
785
-
D-maltose wild-type, pH 7.0 Acinetobacter calcoaceticus
861
-
D-mannose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1015
-
D-maltose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1063
-
2-deoxy-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1443
-
D-allose wild-type, pH 7.0 Acinetobacter calcoaceticus
1448
-
3-O-methyl-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1795
-
D-xylose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
3071
-
D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
3198
-
3-O-methyl-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
3436
-
D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
4563
-
D-allose mutant E277K, pH 7.0 Acinetobacter calcoaceticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4
-
2-deoxy-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
7
-
D-lactose wild-type, pH 7.0 Acinetobacter calcoaceticus
12
-
2-deoxy-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
12
-
D-mannose wild-type, pH 7.0 Acinetobacter calcoaceticus
18
-
D-galactose wild-type, pH 7.0 Acinetobacter calcoaceticus
20
-
D-lactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
25
-
D-maltose wild-type, pH 7.0 Acinetobacter calcoaceticus
31
-
3-O-methyl-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
39
-
D-mannose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
47
-
D-xylose wild-type, pH 7.0 Acinetobacter calcoaceticus
50
-
D-allose wild-type, pH 7.0 Acinetobacter calcoaceticus
71
-
D-maltose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
78
-
D-galactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
118
-
3-O-methyl-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
128
-
D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
239
-
D-xylose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
287
-
D-allose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
349
-
D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus