Literature summary for 1.1.99.35 extracted from

Oubrie, A.; Rozeboom, H.J.; Dijkstra, B.W.
Active-site structure of the soluble quinoprotein glucose dehydrogenase complexed with methylhydrazine: A covalent cofactor-inhibitor complex (1999), Proc. Natl. Acad. Sci. USA, 96, 11787-11791.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
ternary complex of s-GDH with PQQ and methylhydrazine, at 1.5 A resolution. Formation of a covalent PQQ adduct in the active-site. The C5 carbonyl group of the cofactor is the most reactive moiety of PQQ. The binding of the cofactor to s-GDH is predominantly governed by polar interactions. The C2, C7, and C9 carboxyl groups of PQQ form salt bridges with Arg408, Lys377, and Arg406, respectively. The ortho-quinone O4 and O5 atoms are bound by Asn229 and Arg228, respectively. The N6, O7A, and O5 atoms of PQQ are ligands for the active-site calcium ion. The other calcium ligands are provided by the two main chain carbonyl oxygen atoms of Gly-247 and Pro-248 and two watermolecules	Acinetobacter calcoaceticus

Crystallization (Comment)

Organism

ternary complex of s-GDH with PQQ and methylhydrazine, at 1.5 A resolution. Formation of a covalent PQQ adduct in the active-site. The C5 carbonyl group of the cofactor is the most reactive moiety of PQQ. The binding of the cofactor to s-GDH is predominantly governed by polar interactions. The C2, C7, and C9 carboxyl groups of PQQ form salt bridges with Arg408, Lys377, and Arg406, respectively. The ortho-quinone O4 and O5 atoms are bound by Asn229 and Arg228, respectively. The N6, O7A, and O5 atoms of PQQ are ligands for the active-site calcium ion. The other calcium ligands are provided by the two main chain carbonyl oxygen atoms of Gly-247 and Pro-248 and two watermolecules

Acinetobacter calcoaceticus

Inhibitors

Inhibitors	Comment	Organism	Structure
methylhydrazine	competitive inhibitor	Acinetobacter calcoaceticus

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter calcoaceticus	-	-	-

Synonyms

Synonyms	Comment	Organism
s-GDH	-	Acinetobacter calcoaceticus

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Release 2023.1 (January 2023)