Protein Variants | Comment | Organism |
---|---|---|
D340F/Y418F | increase in temperature stability | Acinetobacter calcoaceticus |
D340F/Y418I | increase in temperature stability | Acinetobacter calcoaceticus |
T416V/T417V | increase in temperature stability | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
D-glucose | mutant T416V/T417V, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
20 | - |
D-glucose | wild-type, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
20 | - |
D-glucose | mutant D340F/Y418F, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
20 | - |
D-glucose | mutant D340F/Y418I, pH 7.0, 25°C | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53.9 | - |
wild-type, 50% loss of initial activity within in 10 min | Acinetobacter calcoaceticus |
55 | - |
half-life of wild-type, 9.5 min, of mutant D340F/Y418F 14.9 min, of mutant D340F/Y418I 15.5 min, of mutant T416V/T417V 14.8 min, respectively | Acinetobacter calcoaceticus |
56.5 | - |
mutant T416V/T417V, 50% loss of initial activity within in 10 min | Acinetobacter calcoaceticus |
57.5 | - |
mutant D340F/Y418I, 50% loss of initial activity within in 10 min | Acinetobacter calcoaceticus |
57.7 | - |
mutant D340F/Y418F, 50% loss of initial activity within in 10 min | Acinetobacter calcoaceticus |