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Literature summary for 1.1.99.35 extracted from

  • Tanaka, S.; Igarashi, S.; Ferri, S.; Sode, K.
    Increasing stability of water-soluble PQQ glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface (2005), BMC Biochem., 6, 1.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D340F/Y418F increase in temperature stability Acinetobacter calcoaceticus
D340F/Y418I increase in temperature stability Acinetobacter calcoaceticus
T416V/T417V increase in temperature stability Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16
-
D-glucose mutant T416V/T417V, pH 7.0, 25┬░C Acinetobacter calcoaceticus
20
-
D-glucose wild-type, pH 7.0, 25┬░C Acinetobacter calcoaceticus
20
-
D-glucose mutant D340F/Y418F, pH 7.0, 25┬░C Acinetobacter calcoaceticus
20
-
D-glucose mutant D340F/Y418I, pH 7.0, 25┬░C Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
53.9
-
wild-type, 50% loss of initial activity within in 10 min Acinetobacter calcoaceticus
55
-
half-life of wild-type, 9.5 min, of mutant D340F/Y418F 14.9 min, of mutant D340F/Y418I 15.5 min, of mutant T416V/T417V 14.8 min, respectively Acinetobacter calcoaceticus
56.5
-
mutant T416V/T417V, 50% loss of initial activity within in 10 min Acinetobacter calcoaceticus
57.5
-
mutant D340F/Y418I, 50% loss of initial activity within in 10 min Acinetobacter calcoaceticus
57.7
-
mutant D340F/Y418F, 50% loss of initial activity within in 10 min Acinetobacter calcoaceticus