BRENDA - Enzyme Database
show all sequences of 1.1.99.35

Construction and characterization of heterodimeric soluble quinoprotein glucose dehydrogenase

Igarashi, S.; Sode, K.; J. Biochem. Biophys. Methods 61, 331-338 (2004)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Acinetobacter calcoaceticus
Engineering
Protein Variants
Commentary
Organism
additional information
constructed of heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits. The heterodimeric wild-type/H168Q shows slightly decreased GDH activity and almost identical substrate specificity profile to the wild-type enzyme. The Hill coefficient of the heterodimer is calculated as 1.13, indicating positive cooperativity
Acinetobacter calcoaceticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
3
D-glucose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
2
3
3-O-methyl-D-glucose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
8
-
D-galactose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
9
-
D-galactose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
13
-
maltose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
14
-
maltose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
20
-
lactose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
20
-
lactose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
22
-
D-glucose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
22
-
3-O-methyl-D-glucose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
34
-
D-allose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
36
-
D-allose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
Organism
Organism
UniProt
Commentary
Textmining
Acinetobacter calcoaceticus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
study on heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits
Acinetobacter calcoaceticus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Acinetobacter calcoaceticus
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
constructed of heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits. The heterodimeric wild-type/H168Q shows slightly decreased GDH activity and almost identical substrate specificity profile to the wild-type enzyme. The Hill coefficient of the heterodimer is calculated as 1.13, indicating positive cooperativity
Acinetobacter calcoaceticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
3
D-glucose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
2
3
3-O-methyl-D-glucose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
8
-
D-galactose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
9
-
D-galactose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
13
-
maltose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
14
-
maltose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
20
-
lactose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
20
-
lactose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
22
-
D-glucose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
22
-
3-O-methyl-D-glucose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
34
-
D-allose
recombinant wild-type with R4-tail, pH 7.0
Acinetobacter calcoaceticus
36
-
D-allose
heterodimer wild-type/mutant H168Q, pH 7.0
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
655929
Acinetobacter calcoaceticus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
study on heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits
Acinetobacter calcoaceticus
Other publictions for EC 1.1.99.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739914
An
Regulation of pyrroloquinoline ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
82
4955-4964
2016
-
-
-
-
-
-
-
1
1
1
-
-
-
42
-
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
739848
Flexer
Wired pyrroloquinoline quinone ...
Acinetobacter calcoaceticus
Anal. Chem.
86
2465-2473
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739872
Ben Farhat
Coexpression of the pyrroloqui ...
Serratia marcescens, Serratia marcescens CTM 50650
Appl. Biochem. Biotechnol.
170
1738-1750
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
-
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4
-
1
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-
1
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-
1
1
-
-
-
-
-
-
-
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-
-
-
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-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739873
Stredansky
Monitoring of PQQ-dependent gl ...
Acinetobacter calcoaceticus
Appl. Biochem. Biotechnol.
171
1032-1041
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
13
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
12
12
724836
Schubart
-
A pyrroloquinolinequinone-depe ...
Acinetobacter calcoaceticus
Electrochim. Acta
82
224-232
2012
-
1
-
-
-
-
-
-
-
-
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1
-
1
-
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1
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2
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1
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1
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1
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1
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1
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1
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-
1
-
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-
-
-
-
-
724852
Yu
-
High-level production of solub ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus L.M.D. 79.41
Eng. Life Sci.
12
574-582
2012
-
-
1
-
-
-
-
-
1
-
1
10
-
3
-
-
1
-
-
1
2
-
10
1
2
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
1
10
-
-
-
1
-
1
2
-
10
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
723943
Flexer
Efficient direct electron tran ...
Acinetobacter calcoaceticus
Anal. Chem.
83
5721-5727
2011
-
-
-
-
1
1
-
-
1
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
1
-
-
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-
1
-
1
1
-
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-
-
1
-
-
1
-
-
-
-
-
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-
-
2
-
-
-
-
-
-
-
-
-
-
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-
-
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-
724172
Durand
Designing a highly active solu ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
402
750-754
2010
-
-
1
-
1
-
-
2
-
-
1
1
-
2
-
-
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-
-
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1
1
2
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1
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1
1
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1
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2
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1
1
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-
1
1
-
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-
-
-
-
-
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-
-
-
-
-
-
655127
Tanaka
Increasing stability of water- ...
Acinetobacter calcoaceticus
BMC Biochem.
6
1
2005
-
-
-
-
3
-
-
4
-
-
-
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-
1
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-
1
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5
-
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-
3
-
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4
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-
1
-
-
-
5
-
-
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-
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-
-
-
-
655929
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
J. Biochem. Biophys. Methods
61
331-338
2004
-
-
1
-
1
-
-
12
-
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1
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6
1
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1
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1
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12
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6
1
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-
-
-
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-
654863
Oubrie
Structure and mechanism of sol ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1647
143-151
2003
-
-
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1
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1
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1
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1
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1
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-
654398
Sode
-
Construction of engineered wat ...
Acinetobacter calcoaceticus
Biocatal. Biotransform.
20
405-412
2002
-
-
-
-
5
-
-
18
-
-
-
-
-
1
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-
6
-
2
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18
-
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-
5
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18
-
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6
-
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-
18
-
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18
18
655363
Yoshida
-
Secretion of water soluble pyr ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
30
312-318
2002
-
1
1
-
1
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4
-
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1
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1
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2
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1
1
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1
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4
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1
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2
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639218
Sode
Increasing the thermal stabili ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
26
491-496
2000
-
-
-
-
7
-
-
2
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1
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2
-
9
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1
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7
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2
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2
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9
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1
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702202
Dewanti
Ca2+-assisted, direct hydride ...
Acinetobacter calcoaceticus
Biochemistry
39
9384-9392
2000
-
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4
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1
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1
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1
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1
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702811
Kojima
-
The production of soluble pyrr ...
Acinetobacter calcoaceticus
Biotechnol. Lett.
22
1343-1347
2000
-
1
1
-
-
-
-
-
-
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1
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1
1
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639204
Oubrie
The 1.7 A crystal structure of ...
Acinetobacter calcoaceticus
J. Mol. Biol.
289
319-333
1999
-
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1
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1
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4
-
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1
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1
1
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1
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639216
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
264
820-824
1999
-
-
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12
-
-
18
-
-
-
-
-
1
-
-
-
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-
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-
9
-
-
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-
-
18
-
-
-
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9
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Negative cooperativity in the ...
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Ca2+ and its substitutes have ...
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