Cloned (Comment) | Organism |
---|---|
- |
Acinetobacter calcoaceticus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | constructed of heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits. The heterodimeric wild-type/H168Q shows slightly decreased GDH activity and almost identical substrate specificity profile to the wild-type enzyme. The Hill coefficient of the heterodimer is calculated as 1.13, indicating positive cooperativity | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | D-glucose | recombinant wild-type with R4-tail, pH 7.0 | Acinetobacter calcoaceticus | |
2 | 3 | 3-O-methyl-D-glucose | recombinant wild-type with R4-tail, pH 7.0 | Acinetobacter calcoaceticus | |
8 | - |
D-galactose | recombinant wild-type with R4-tail, pH 7.0 | Acinetobacter calcoaceticus | |
9 | - |
D-galactose | heterodimer wild-type/mutant H168Q, pH 7.0 | Acinetobacter calcoaceticus | |
13 | - |
maltose | heterodimer wild-type/mutant H168Q, pH 7.0 | Acinetobacter calcoaceticus | |
14 | - |
maltose | recombinant wild-type with R4-tail, pH 7.0 | Acinetobacter calcoaceticus | |
20 | - |
lactose | heterodimer wild-type/mutant H168Q, pH 7.0 | Acinetobacter calcoaceticus | |
20 | - |
lactose | recombinant wild-type with R4-tail, pH 7.0 | Acinetobacter calcoaceticus | |
22 | - |
D-glucose | heterodimer wild-type/mutant H168Q, pH 7.0 | Acinetobacter calcoaceticus | |
22 | - |
3-O-methyl-D-glucose | heterodimer wild-type/mutant H168Q, pH 7.0 | Acinetobacter calcoaceticus | |
34 | - |
D-allose | recombinant wild-type with R4-tail, pH 7.0 | Acinetobacter calcoaceticus | |
36 | - |
D-allose | heterodimer wild-type/mutant H168Q, pH 7.0 | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-allose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-galactose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | study on heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits | Acinetobacter calcoaceticus |