BRENDA - Enzyme Database
show all sequences of 1.1.99.35

Negative cooperativity in the steady-state kinetics of sugar oxidation by soluble quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus

Olsthoorn, A.J.J.; Otsuki, T.; Duine, J.A.; Eur. J. Biochem. 255, 255-261 (1998)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-glucose
-
Acinetobacter calcoaceticus
lactose
-
Acinetobacter calcoaceticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
under conditions that avoid its masking by sugar-substrate inhibition as much as possible, negative kinetic cooperativity with respect to sugar substrate oxidation is observed. The cooperativity effect dramatically changes the performance of soluble GDH, as reflected by the V2 and K2 values for glucose in phosphate buffer being about 10-fold and 100-fold higher than the V1 and K1 values, respectively. Substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose
Acinetobacter calcoaceticus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose
Acinetobacter calcoaceticus
Sr2+
substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose
Acinetobacter calcoaceticus
Organism
Organism
UniProt
Commentary
Textmining
Acinetobacter calcoaceticus
-
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
D-glucose + acceptor = D-glucono-1,5-lactone + reduced acceptor
ping-pong behaviour and double-substrate inhibition
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-galactose + oxidized N-methylphenazonium methyl sulfate
-
703532
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
?
D-glucose + oxidized N-methylphenazonium methyl sulfate
-
703532
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
?
D-xylose + oxidized N-methylphenazonium methyl sulfate
-
703532
Acinetobacter calcoaceticus
D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
?
lactose + oxidized N-methylphenazonium methyl sulfate
-
703532
Acinetobacter calcoaceticus
4-O-beta-D-galactopyranosyl-D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
?
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
61
-
lactose
pH 7.0, 20°C
Acinetobacter calcoaceticus
92
-
D-glucose
pH 7.0, 20°C
Acinetobacter calcoaceticus
156
-
D-glucose
enzyme containing Sr2+ instead of Ca2+, pH 7.0, 20°C
Acinetobacter calcoaceticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-glucose
-
Acinetobacter calcoaceticus
lactose
-
Acinetobacter calcoaceticus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
61
-
lactose
pH 7.0, 20°C
Acinetobacter calcoaceticus
92
-
D-glucose
pH 7.0, 20°C
Acinetobacter calcoaceticus
156
-
D-glucose
enzyme containing Sr2+ instead of Ca2+, pH 7.0, 20°C
Acinetobacter calcoaceticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
under conditions that avoid its masking by sugar-substrate inhibition as much as possible, negative kinetic cooperativity with respect to sugar substrate oxidation is observed. The cooperativity effect dramatically changes the performance of soluble GDH, as reflected by the V2 and K2 values for glucose in phosphate buffer being about 10-fold and 100-fold higher than the V1 and K1 values, respectively. Substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose
Acinetobacter calcoaceticus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose
Acinetobacter calcoaceticus
Sr2+
substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-galactose + oxidized N-methylphenazonium methyl sulfate
-
703532
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
?
D-glucose + oxidized N-methylphenazonium methyl sulfate
-
703532
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
?
D-xylose + oxidized N-methylphenazonium methyl sulfate
-
703532
Acinetobacter calcoaceticus
D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
?
lactose + oxidized N-methylphenazonium methyl sulfate
-
703532
Acinetobacter calcoaceticus
4-O-beta-D-galactopyranosyl-D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
?
Other publictions for EC 1.1.99.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739914
An
Regulation of pyrroloquinoline ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
82
4955-4964
2016
-
-
-
-
-
-
-
1
1
1
-
-
-
42
-
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
739848
Flexer
Wired pyrroloquinoline quinone ...
Acinetobacter calcoaceticus
Anal. Chem.
86
2465-2473
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739872
Ben Farhat
Coexpression of the pyrroloqui ...
Serratia marcescens, Serratia marcescens CTM 50650
Appl. Biochem. Biotechnol.
170
1738-1750
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739873
Stredansky
Monitoring of PQQ-dependent gl ...
Acinetobacter calcoaceticus
Appl. Biochem. Biotechnol.
171
1032-1041
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
13
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
12
12
724836
Schubart
-
A pyrroloquinolinequinone-depe ...
Acinetobacter calcoaceticus
Electrochim. Acta
82
224-232
2012
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
724852
Yu
-
High-level production of solub ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus L.M.D. 79.41
Eng. Life Sci.
12
574-582
2012
-
-
1
-
-
-
-
-
1
-
1
10
-
3
-
-
1
-
-
1
2
-
10
1
2
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
1
10
-
-
-
1
-
1
2
-
10
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
723943
Flexer
Efficient direct electron tran ...
Acinetobacter calcoaceticus
Anal. Chem.
83
5721-5727
2011
-
-
-
-
1
1
-
-
1
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724172
Durand
Designing a highly active solu ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
402
750-754
2010
-
-
1
-
1
-
-
2
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655127
Tanaka
Increasing stability of water- ...
Acinetobacter calcoaceticus
BMC Biochem.
6
1
2005
-
-
-
-
3
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
655929
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
J. Biochem. Biophys. Methods
61
331-338
2004
-
-
1
-
1
-
-
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654863
Oubrie
Structure and mechanism of sol ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1647
143-151
2003
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654398
Sode
-
Construction of engineered wat ...
Acinetobacter calcoaceticus
Biocatal. Biotransform.
20
405-412
2002
-
-
-
-
5
-
-
18
-
-
-
-
-
1
-
-
-
-
-
-
-
-
6
-
2
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
18
-
-
-
-
-
-
-
-
18
18
655363
Yoshida
-
Secretion of water soluble pyr ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
30
312-318
2002
-
1
1
-
1
-
-
4
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639218
Sode
Increasing the thermal stabili ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
26
491-496
2000
-
-
-
-
7
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
2
-
9
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
702202
Dewanti
Ca2+-assisted, direct hydride ...
Acinetobacter calcoaceticus
Biochemistry
39
9384-9392
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702811
Kojima
-
The production of soluble pyrr ...
Acinetobacter calcoaceticus
Biotechnol. Lett.
22
1343-1347
2000
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639204
Oubrie
The 1.7 A crystal structure of ...
Acinetobacter calcoaceticus
J. Mol. Biol.
289
319-333
1999
-
-
-
1
-
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639216
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
264
820-824
1999
-
-
-
-
12
-
-
18
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
18
-
-
-
-
-
-
-
-
18
18
639220
Oubrie
Active-site structure of the s ...
Acinetobacter calcoaceticus
Proc. Natl. Acad. Sci. USA
96
11787-11791
1999
-
-
-
1
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639209
Olsthoorn
On the mechanism and specifici ...
Acinetobacter calcoaceticus
Biochemistry
37
13854-13861
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
16
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
1
-
-
-
-
-
-
-
17
17
703532
Olsthoorn
Negative cooperativity in the ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
255
255-261
1998
-
-
-
-
-
-
2
1
-
2
-
-
-
6
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
3
1
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703530
Olsthoorn
Ca2+ and its substitutes have ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
247
659-665
1997
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702726
Matsushita
-
Soluble and membrane-bound qui ...
Acinetobacter calcoaceticus
Biosci. Biotechnol. Biochem.
59
1548-1555
1995
-
-
-
-
-
-
-
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705107
Schlunegger
Crystallization and preliminar ...
Acinetobacter calcoaceticus
J. Mol. Biol.
233
784-786
1993
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-