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Literature summary for 1.1.99.35 extracted from

  • Olsthoorn, A.J.J.; Otsuki, T.; Duine, J.A.
    Negative cooperativity in the steady-state kinetics of sugar oxidation by soluble quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus (1998), Eur. J. Biochem., 255, 255-261.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-glucose
-
Acinetobacter calcoaceticus
lactose
-
Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information under conditions that avoid its masking by sugar-substrate inhibition as much as possible, negative kinetic cooperativity with respect to sugar substrate oxidation is observed. The cooperativity effect dramatically changes the performance of soluble GDH, as reflected by the V2 and K2 values for glucose in phosphate buffer being about 10-fold and 100-fold higher than the V1 and K1 values, respectively. Substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose Acinetobacter calcoaceticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose Acinetobacter calcoaceticus
Sr2+ substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
D-glucose + acceptor = D-glucono-1,5-lactone + reduced acceptor ping-pong behaviour and double-substrate inhibition Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + oxidized N-methylphenazonium methyl sulfate
-
Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
?
D-glucose + oxidized N-methylphenazonium methyl sulfate
-
Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
?
D-xylose + oxidized N-methylphenazonium methyl sulfate
-
Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
?
lactose + oxidized N-methylphenazonium methyl sulfate
-
Acinetobacter calcoaceticus 4-O-beta-D-galactopyranosyl-D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
61
-
lactose pH 7.0, 20┬░C Acinetobacter calcoaceticus
92
-
D-glucose pH 7.0, 20┬░C Acinetobacter calcoaceticus
156
-
D-glucose enzyme containing Sr2+ instead of Ca2+, pH 7.0, 20┬░C Acinetobacter calcoaceticus