High-level production of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase in Escherichia coli

Data extracted from this reference:

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

D-galactose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

about 13% activity compared to D-glucose

D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

D-galactose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus L.M.D. 79.41

about 13% activity compared to D-glucose

D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

D-glucose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

100% activity

D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

D-glucose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus L.M.D. 79.41

100% activity

D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

D-mannose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

about 11% activity compared to D-glucose

D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

D-xylose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

about 9% activity compared to D-glucose

D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

lactose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

about 50% activity compared to D-glucose

lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

maltose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

about 70% activity compared to D-glucose

maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

additional information

Acinetobacter calcoaceticus

almost no activity with D-fructose

?

-

-

?

additional information

Acinetobacter calcoaceticus L.M.D. 79.41

almost no activity with D-fructose

?

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

D-galactose + oxidized 2,6-dichlorophenolindophenol

about 13% activity compared to D-glucose

724852

Acinetobacter calcoaceticus

D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

D-galactose + oxidized 2,6-dichlorophenolindophenol

about 13% activity compared to D-glucose

724852

Acinetobacter calcoaceticus L.M.D. 79.41

D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

D-glucose + oxidized 2,6-dichlorophenolindophenol

100% activity

724852

Acinetobacter calcoaceticus

D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

D-glucose + oxidized 2,6-dichlorophenolindophenol

100% activity

724852

Acinetobacter calcoaceticus L.M.D. 79.41

D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

D-mannose + oxidized 2,6-dichlorophenolindophenol

about 11% activity compared to D-glucose

724852

Acinetobacter calcoaceticus

D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

D-xylose + oxidized 2,6-dichlorophenolindophenol

about 9% activity compared to D-glucose

724852

Acinetobacter calcoaceticus

D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

lactose + oxidized 2,6-dichlorophenolindophenol

about 50% activity compared to D-glucose

724852

Acinetobacter calcoaceticus

lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

maltose + oxidized 2,6-dichlorophenolindophenol

about 70% activity compared to D-glucose

724852

Acinetobacter calcoaceticus

maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

additional information

almost no activity with D-fructose

724852

Acinetobacter calcoaceticus

?

-

-

-

?

additional information

almost no activity with D-fructose

724852

Acinetobacter calcoaceticus L.M.D. 79.41

?

-

-

-

?

Cofactor

Commentary

Organism

Structure

pyrroloquinoline quinone

-

Acinetobacter calcoaceticus

Cofactor

Commentary

Organism

Structure

pyrroloquinoline quinone

-

Acinetobacter calcoaceticus

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

D-galactose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

about 13% activity compared to D-glucose

D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

D-galactose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus L.M.D. 79.41

about 13% activity compared to D-glucose

D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

D-glucose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

100% activity

D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

D-glucose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus L.M.D. 79.41

100% activity

D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

D-mannose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

about 11% activity compared to D-glucose

D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

D-xylose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

about 9% activity compared to D-glucose

D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

lactose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

about 50% activity compared to D-glucose

lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

maltose + oxidized 2,6-dichlorophenolindophenol

Acinetobacter calcoaceticus

about 70% activity compared to D-glucose

maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

?

additional information

Acinetobacter calcoaceticus

almost no activity with D-fructose

?

-

-

?

additional information

Acinetobacter calcoaceticus L.M.D. 79.41

almost no activity with D-fructose

?

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

D-galactose + oxidized 2,6-dichlorophenolindophenol

about 13% activity compared to D-glucose

724852

Acinetobacter calcoaceticus

D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

D-galactose + oxidized 2,6-dichlorophenolindophenol

about 13% activity compared to D-glucose

724852

Acinetobacter calcoaceticus L.M.D. 79.41

D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

D-glucose + oxidized 2,6-dichlorophenolindophenol

100% activity

724852

Acinetobacter calcoaceticus

D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

D-glucose + oxidized 2,6-dichlorophenolindophenol

100% activity

724852

Acinetobacter calcoaceticus L.M.D. 79.41

D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

D-mannose + oxidized 2,6-dichlorophenolindophenol

about 11% activity compared to D-glucose

724852

Acinetobacter calcoaceticus

D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

D-xylose + oxidized 2,6-dichlorophenolindophenol

about 9% activity compared to D-glucose

724852

Acinetobacter calcoaceticus

D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

lactose + oxidized 2,6-dichlorophenolindophenol

about 50% activity compared to D-glucose

724852

Acinetobacter calcoaceticus

lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

maltose + oxidized 2,6-dichlorophenolindophenol

about 70% activity compared to D-glucose

724852

Acinetobacter calcoaceticus

maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol

-

-

-

?

additional information

almost no activity with D-fructose

724852

Acinetobacter calcoaceticus

?

-

-

-

?

additional information

almost no activity with D-fructose

724852

Acinetobacter calcoaceticus L.M.D. 79.41

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

739914

An

Regulation of pyrroloquinoline ...

Pseudomonas putida, Pseudomonas putida KT 2240

Appl. Environ. Microbiol.

82

4955-4964

2016

-

-

-

-

-

-

-

1

1

1

-

-

-

42

-

-

-

-

-

-

-

-

4

-

3

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

1

1

1

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

739848

Flexer

Wired pyrroloquinoline quinone ...

Acinetobacter calcoaceticus

Anal. Chem.

86

2465-2473

2014

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

1

-

2

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

739872

Ben Farhat

Coexpression of the pyrroloqui ...

Serratia marcescens, Serratia marcescens CTM 50650

Appl. Biochem. Biotechnol.

170

1738-1750

2013

-

-

1

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

4

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

739873

Stredansky

Monitoring of PQQ-dependent gl ...

Acinetobacter calcoaceticus

Appl. Biochem. Biotechnol.

171

1032-1041

2013

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

13

-

3

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

13

-

-

-

-

-

-

-

-

-

-

-

-

-

12

12

724836

Schubart

-

A pyrroloquinolinequinone-depe ...

Acinetobacter calcoaceticus

Electrochim. Acta

82

224-232

2012

-

1

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

1

-

2

-

-

-

-

1

-

-

1

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

724852

Yu

-

High-level production of solub ...

Acinetobacter calcoaceticus, Acinetobacter calcoaceticus L.M.D. 79.41

Eng. Life Sci.

12

574-582

2012

-

-

1

-

-

-

-

-

1

-

1

10

-

3

-

-

1

-

-

1

2

-

10

1

2

-

-

1

-

-

-

-

1

-

-

-

-

-

1

1

-

-

-

-

-

-

-

1

-

1

10

-

-

-

1

-

1

2

-

10

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

723943

Flexer

Efficient direct electron tran ...

Acinetobacter calcoaceticus

Anal. Chem.

83

5721-5727

2011

-

-

-

-

1

1

-

-

1

-

-

1

-

5

-

-

-

-

-

-

-

-

2

-

3

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

1

1

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

724172

Durand

Designing a highly active solu ...

Acinetobacter calcoaceticus

Biochem. Biophys. Res. Commun.

402

750-754

2010

-

-

1

-

1

-

-

2

-

-

1

1

-

2

-

-

-

-

-

-

-

-

1

1

2

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

1

-

-

-

-

2

-

-

1

1

-

-

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

655127

Tanaka

Increasing stability of water- ...

Acinetobacter calcoaceticus

BMC Biochem.

6

1

2005

-

-

-

-

3

-

-

4

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

5

-

-

-

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

5

-

-

-

-

-

-

-

-

-

-

-

655929

Igarashi

Construction and characterizat ...

Acinetobacter calcoaceticus

J. Biochem. Biophys. Methods

61

331-338

2004

-

-

1

-

1

-

-

12

-

-

-

-

-

1

-

-

-

-

-

-

-

-

6

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

12

-

-

-

-

-

-

-

-

-

-

-

-

6

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

654863

Oubrie

Structure and mechanism of sol ...

Acinetobacter calcoaceticus

Biochim. Biophys. Acta

1647

143-151

2003

-

-

-

1

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

654398

Sode

-

Construction of engineered wat ...

Acinetobacter calcoaceticus

Biocatal. Biotransform.

20

405-412

2002

-

-

-

-

5

-

-

18

-

-

-

-

-

1

-

-

-

-

-

-

-

-

6

-

2

-

-

-

18

-

-

-

-

-

-

-

-

-

-

-

-

5

-

-

-

-

18

-

-

-

-

-

-

-

-

-

-

-

-

6

-

-

-

-

18

-

-

-

-

-

-

-

-

18

18

655363

Yoshida

-

Secretion of water soluble pyr ...

Acinetobacter calcoaceticus

Enzyme Microb. Technol.

30

312-318

2002

-

1

1

-

1

-

-

4

-

-

-

-

-

1

-

1

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

1

-

-

-

-

4

-

-

-

-

-

-

1

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

639218

Sode

Increasing the thermal stabili ...

Acinetobacter calcoaceticus

Enzyme Microb. Technol.

26

491-496

2000

-

-

-

-

7

-

-

2

-

-

-

-

-

1

-

-

-

-

-

-

2

-

9

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

7

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

2

-

9

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

702202

Dewanti

Ca2+-assisted, direct hydride ...

Acinetobacter calcoaceticus

Biochemistry

39

9384-9392

2000

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

702811

Kojima

-

The production of soluble pyrr ...

Acinetobacter calcoaceticus

Biotechnol. Lett.

22

1343-1347

2000

-

1

1

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

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1

1

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639204

Oubrie

The 1.7 A crystal structure of ...

Acinetobacter calcoaceticus

J. Mol. Biol.

289

319-333

1999

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1

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1

1

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1

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639216

Igarashi

Construction and characterizat ...

Acinetobacter calcoaceticus

Biochem. Biophys. Res. Commun.

264

820-824

1999

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-

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12

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18

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1

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9

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18

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12

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18

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9

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18

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18

18

639220

Oubrie

Active-site structure of the s ...

Acinetobacter calcoaceticus

Proc. Natl. Acad. Sci. USA

96

11787-11791

1999

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1

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1

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3

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1

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1

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1

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639209

Olsthoorn

On the mechanism and specifici ...

Acinetobacter calcoaceticus

Biochemistry

37

13854-13861

1998

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2

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16

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1

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1

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16

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1

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17

17

703532

Olsthoorn

Negative cooperativity in the ...

Acinetobacter calcoaceticus

Eur. J. Biochem.

255

255-261

1998

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2

1

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6

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1

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3

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3

1

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2

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703530

Olsthoorn

Ca2+ and its substitutes have ...

Acinetobacter calcoaceticus

Eur. J. Biochem.

247

659-665

1997

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702726

Matsushita

-

Soluble and membrane-bound qui ...

Acinetobacter calcoaceticus

Biosci. Biotechnol. Biochem.

59

1548-1555

1995

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5

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1

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1

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1

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1

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705107

Schlunegger

Crystallization and preliminar ...

Acinetobacter calcoaceticus

J. Mol. Biol.

233

784-786

1993

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1

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1

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