BRENDA - Enzyme Database show
show all sequences of 1.1.99.35

High-level production of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase in Escherichia coli

Yu, Y.; Wei, P.; Zhu, X.; Huang, L.; Cai, J.; Xu, Z.; Eng. Life Sci. 12, 574-582 (2012)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Acinetobacter calcoaceticus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Acinetobacter calcoaceticus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
2 * 50000, SDS-PAGE
Acinetobacter calcoaceticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galactose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
about 13% activity compared to D-glucose
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus L.M.D. 79.41
about 13% activity compared to D-glucose
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
100% activity
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus L.M.D. 79.41
100% activity
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
about 11% activity compared to D-glucose
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
about 9% activity compared to D-glucose
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
about 50% activity compared to D-glucose
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
about 70% activity compared to D-glucose
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
additional information
Acinetobacter calcoaceticus
almost no activity with D-fructose
?
-
-
-
additional information
Acinetobacter calcoaceticus L.M.D. 79.41
almost no activity with D-fructose
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter calcoaceticus
-
-
-
Acinetobacter calcoaceticus L.M.D. 79.41
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA affinity column chromatography
Acinetobacter calcoaceticus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:D-glucose-grown cell
-
Acinetobacter calcoaceticus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
515
-
crude cell lysate, at pH 7.0 and 25C
Acinetobacter calcoaceticus
5811
-
after 11.3fold purification, at pH 7.0 and 25C
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galactose + oxidized 2,6-dichlorophenolindophenol
about 13% activity compared to D-glucose
724852
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
about 13% activity compared to D-glucose
724852
Acinetobacter calcoaceticus L.M.D. 79.41
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
100% activity
724852
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
100% activity
724852
Acinetobacter calcoaceticus L.M.D. 79.41
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
about 11% activity compared to D-glucose
724852
Acinetobacter calcoaceticus
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
about 9% activity compared to D-glucose
724852
Acinetobacter calcoaceticus
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
about 50% activity compared to D-glucose
724852
Acinetobacter calcoaceticus
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
about 70% activity compared to D-glucose
724852
Acinetobacter calcoaceticus
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
additional information
almost no activity with D-fructose
724852
Acinetobacter calcoaceticus
?
-
-
-
-
additional information
almost no activity with D-fructose
724852
Acinetobacter calcoaceticus L.M.D. 79.41
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 50000, SDS-PAGE
Acinetobacter calcoaceticus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
the half-life of enzyme thermal inactivation at 55C is 12.1 min
Acinetobacter calcoaceticus
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Acinetobacter calcoaceticus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Acinetobacter calcoaceticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Acinetobacter calcoaceticus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Acinetobacter calcoaceticus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
2 * 50000, SDS-PAGE
Acinetobacter calcoaceticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galactose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
about 13% activity compared to D-glucose
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus L.M.D. 79.41
about 13% activity compared to D-glucose
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
100% activity
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus L.M.D. 79.41
100% activity
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
about 11% activity compared to D-glucose
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
about 9% activity compared to D-glucose
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
about 50% activity compared to D-glucose
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
Acinetobacter calcoaceticus
about 70% activity compared to D-glucose
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
?
additional information
Acinetobacter calcoaceticus
almost no activity with D-fructose
?
-
-
-
additional information
Acinetobacter calcoaceticus L.M.D. 79.41
almost no activity with D-fructose
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA affinity column chromatography
Acinetobacter calcoaceticus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:D-glucose-grown cell
-
Acinetobacter calcoaceticus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
515
-
crude cell lysate, at pH 7.0 and 25C
Acinetobacter calcoaceticus
5811
-
after 11.3fold purification, at pH 7.0 and 25C
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galactose + oxidized 2,6-dichlorophenolindophenol
about 13% activity compared to D-glucose
724852
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
about 13% activity compared to D-glucose
724852
Acinetobacter calcoaceticus L.M.D. 79.41
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
100% activity
724852
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
100% activity
724852
Acinetobacter calcoaceticus L.M.D. 79.41
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
about 11% activity compared to D-glucose
724852
Acinetobacter calcoaceticus
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
about 9% activity compared to D-glucose
724852
Acinetobacter calcoaceticus
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
about 50% activity compared to D-glucose
724852
Acinetobacter calcoaceticus
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
about 70% activity compared to D-glucose
724852
Acinetobacter calcoaceticus
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
additional information
almost no activity with D-fructose
724852
Acinetobacter calcoaceticus
?
-
-
-
-
additional information
almost no activity with D-fructose
724852
Acinetobacter calcoaceticus L.M.D. 79.41
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 50000, SDS-PAGE
Acinetobacter calcoaceticus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
the half-life of enzyme thermal inactivation at 55C is 12.1 min
Acinetobacter calcoaceticus
Other publictions for EC 1.1.99.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739914
An
Regulation of pyrroloquinoline ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
82
4955-4964
2016
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-
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1
1
1
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42
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4
-
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1
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1
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-
1
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
739848
Flexer
Wired pyrroloquinoline quinone ...
Acinetobacter calcoaceticus
Anal. Chem.
86
2465-2473
2014
-
-
-
-
-
-
-
-
-
-
-
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-
2
-
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1
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1
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1
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1
-
-
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-
-
-
-
-
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-
-
-
-
-
739872
Ben Farhat
Coexpression of the pyrroloqui ...
Serratia marcescens, Serratia marcescens CTM 50650
Appl. Biochem. Biotechnol.
170
1738-1750
2013
-
-
1
-
-
-
-
-
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-
2
-
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4
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1
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1
1
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-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739873
Stredansky
Monitoring of PQQ-dependent gl ...
Acinetobacter calcoaceticus
Appl. Biochem. Biotechnol.
171
1032-1041
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
12
12
724836
Schubart
-
A pyrroloquinolinequinone-depe ...
Acinetobacter calcoaceticus
Electrochim. Acta
82
224-232
2012
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
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-
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1
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1
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1
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1
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1
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1
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-
1
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
724852
Yu
-
High-level production of solub ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus L.M.D. 79.41
Eng. Life Sci.
12
574-582
2012
-
-
1
-
-
-
-
-
1
-
1
10
-
3
-
-
1
-
-
1
2
-
10
1
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
1
10
-
-
-
1
-
1
2
-
10
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
723943
Flexer
Efficient direct electron tran ...
Acinetobacter calcoaceticus
Anal. Chem.
83
5721-5727
2011
-
-
-
-
1
1
-
-
1
-
-
1
-
2
-
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2
-
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1
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1
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1
1
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1
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1
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-
2
-
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-
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-
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-
724172
Durand
Designing a highly active solu ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
402
750-754
2010
-
-
1
-
1
-
-
2
-
-
1
1
-
1
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-
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1
1
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1
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1
1
-
1
-
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-
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2
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1
1
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-
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-
-
1
1
-
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-
-
-
-
-
-
-
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-
655127
Tanaka
Increasing stability of water- ...
Acinetobacter calcoaceticus
BMC Biochem.
6
1
2005
-
-
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-
3
-
-
2
-
-
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-
1
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-
-
-
-
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-
-
1
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-
5
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-
3
-
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-
-
2
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-
1
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-
-
5
-
-
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-
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-
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-
-
655929
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
J. Biochem. Biophys. Methods
61
331-338
2004
-
-
1
-
1
-
-
11
-
-
-
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1
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-
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-
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-
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-
6
1
-
-
-
-
-
-
-
-
-
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-
1
-
-
1
-
-
-
-
11
-
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-
-
-
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-
6
1
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-
-
-
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-
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-
-
654863
Oubrie
Structure and mechanism of sol ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1647
143-151
2003
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1
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1
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1
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1
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1
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-
654398
Sode
-
Construction of engineered wat ...
Acinetobacter calcoaceticus
Biocatal. Biotransform.
20
405-412
2002
-
-
-
-
5
-
-
18
-
-
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-
1
-
-
-
-
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6
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18
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5
-
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18
-
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-
6
-
-
-
-
18
-
-
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-
-
-
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-
18
18
655363
Yoshida
-
Secretion of water soluble pyr ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
30
312-318
2002
-
1
1
-
1
-
-
4
-
-
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1
-
1
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2
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1
1
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1
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4
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1
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2
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-
639218
Sode
Increasing the thermal stabili ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
26
491-496
2000
-
-
-
-
7
-
-
2
-
-
-
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1
-
-
-
-
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-
2
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9
-
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1
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7
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2
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2
-
9
-
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1
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-
702202
Dewanti
Ca2+-assisted, direct hydride ...
Acinetobacter calcoaceticus
Biochemistry
39
9384-9392
2000
-
-
-
-
-
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2
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1
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1
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1
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1
-
-
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-
702811
Kojima
-
The production of soluble pyrr ...
Acinetobacter calcoaceticus
Biotechnol. Lett.
22
1343-1347
2000
-
1
1
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1
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1
1
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639204
Oubrie
The 1.7 A crystal structure of ...
Acinetobacter calcoaceticus
J. Mol. Biol.
289
319-333
1999
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-
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1
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1
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2
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1
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1
1
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1
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639216
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
264
820-824
1999
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-
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12
-
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16
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1
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9
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18
-
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12
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16
-
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9
-
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18
-
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-
-
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18
18
639220
Oubrie
Active-site structure of the s ...
Acinetobacter calcoaceticus
Proc. Natl. Acad. Sci. USA
96
11787-11791
1999
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-
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1
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1
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2
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1
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1
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639209
Olsthoorn
On the mechanism and specifici ...
Acinetobacter calcoaceticus
Biochemistry
37
13854-13861
1998
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-
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1
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16
-
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1
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16
-
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1
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17
17
703532
Olsthoorn
Negative cooperativity in the ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
255
255-261
1998
-
-
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2
1
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2
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2
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1
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4
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3
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2
3
1
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2
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4
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703530
Olsthoorn
Ca2+ and its substitutes have ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
247
659-665
1997
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-
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1
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1
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1
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702726
Matsushita
-
Soluble and membrane-bound qui ...
Acinetobacter calcoaceticus
Biosci. Biotechnol. Biochem.
59
1548-1555
1995
-
-
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5
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1
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1
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1
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5
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-
705107
Schlunegger
Crystallization and preliminar ...
Acinetobacter calcoaceticus
J. Mol. Biol.
233
784-786
1993
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1
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1
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