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Literature summary for 1.1.99.35 extracted from

  • Yu, Y.; Wei, P.; Zhu, X.; Huang, L.; Cai, J.; Xu, Z.
    High-level production of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase in Escherichia coli (2012), Eng. Life Sci., 12, 574-582.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acinetobacter calcoaceticus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Acinetobacter calcoaceticus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose + oxidized 2,6-dichlorophenolindophenol Acinetobacter calcoaceticus about 13% activity compared to D-glucose D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol Acinetobacter calcoaceticus L.M.D. 79.41 about 13% activity compared to D-glucose D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol Acinetobacter calcoaceticus 100% activity D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol Acinetobacter calcoaceticus L.M.D. 79.41 100% activity D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol Acinetobacter calcoaceticus about 11% activity compared to D-glucose D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol Acinetobacter calcoaceticus about 9% activity compared to D-glucose D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
lactose + oxidized 2,6-dichlorophenolindophenol Acinetobacter calcoaceticus about 50% activity compared to D-glucose lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol Acinetobacter calcoaceticus about 70% activity compared to D-glucose maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
additional information Acinetobacter calcoaceticus almost no activity with D-fructose ?
-
?
additional information Acinetobacter calcoaceticus L.M.D. 79.41 almost no activity with D-fructose ?
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-
Acinetobacter calcoaceticus L.M.D. 79.41
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Acinetobacter calcoaceticus

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:D-glucose-grown cell
-
Acinetobacter calcoaceticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
515
-
crude cell lysate, at pH 7.0 and 25┬░C Acinetobacter calcoaceticus
5811
-
after 11.3fold purification, at pH 7.0 and 25┬░C Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + oxidized 2,6-dichlorophenolindophenol about 13% activity compared to D-glucose Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol about 13% activity compared to D-glucose Acinetobacter calcoaceticus L.M.D. 79.41 D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol 100% activity Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol 100% activity Acinetobacter calcoaceticus L.M.D. 79.41 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol about 11% activity compared to D-glucose Acinetobacter calcoaceticus D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol about 9% activity compared to D-glucose Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
lactose + oxidized 2,6-dichlorophenolindophenol about 50% activity compared to D-glucose Acinetobacter calcoaceticus lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol about 70% activity compared to D-glucose Acinetobacter calcoaceticus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
additional information almost no activity with D-fructose Acinetobacter calcoaceticus ?
-
?
additional information almost no activity with D-fructose Acinetobacter calcoaceticus L.M.D. 79.41 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 50000, SDS-PAGE Acinetobacter calcoaceticus

Synonyms

Synonyms Comment Organism
PQQGDH
-
Acinetobacter calcoaceticus
soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
-
Acinetobacter calcoaceticus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
55
-
the half-life of enzyme thermal inactivation at 55┬░C is 12.1 min Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
Acinetobacter calcoaceticus