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Literature summary for 1.1.99.35 extracted from
- Regulation of pyrroloquinoline quinone-dependent glucose dehydrogenase activity in the model rhizosphere-dwelling bacterium Pseudomonas putida KT2440 (2016), Appl. Environ. Microbiol., 82, 4955-4964.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.91 | - |
D-glucose | at pH 7.0 and 25°C | Pseudomonas putida |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Pseudomonas putida | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.5 mM used in assay conditions | Pseudomonas putida | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida KT 2240 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida KT 2240 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + phenazine methosulfate | - |
Pseudomonas putida | D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
| D-glucose + phenazine methosulfate | - |
Pseudomonas putida KT 2240 | D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GCD | - |
Pseudomonas putida |
| GDH | - |
Pseudomonas putida |
| pyrroloquinoline quinone-dependent glucose dehydrogenase | - |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | - |
Pseudomonas putida | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pseudomonas putida | the gene expression level is approximately 1.5-3fold higher in the zero-soluble-phosphate condition than in high-soluble-phosphate condition. Enzyme expression is highest with D-glucose in the growth medium | up |
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Release 2023.1 (January 2023)
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