Crystallization (Comment) | Organism |
---|---|
analysis of electron paramagnetic resonance signals for free enzyme, the enzyme-S-adenosyl-L-methionine complex, and a rhombic signal presumably derived from the enzyme-S-adenosyl-L-methionine-substrate ternary complex show close interaction of both S-adenosyl-L-methione and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster | Niallia circulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
S-adenosyl-L-methionine | pH 8.0, 28°C, EDTA-treated enzyme | Niallia circulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | close interaction of both S-adenosyl-L-methionine and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster | Niallia circulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | Q8G907 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine | radical S-adenosyl-L-methionine enzyme. Ketone formation is the rate-determining step in this reaction. Reaction involves formation of a 2-deoxy-scyllo-inosamine radical which can easily be converted to a highly reductive ketyl radical by deprotonation through proton-coupled electron transfer | Niallia circulans | 3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BtrN | - |
Niallia circulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
S-adenosyl-L-methionine | pH 8.0, 28°C, EDTA-treated enzyme | Niallia circulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Fe-S center | one [4Fe-4S] cluster per monomer, close interaction of both S-adenosyl-L-methione and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster | Niallia circulans |