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Literature summary for 1.1.99.38 extracted from

  • Yokoyama, K.; Ohmori, D.; Kudo, F.; Eguchi, T.
    Mechanistic study on the reaction of a radical SAM dehydrogenase BtrN by electron paramagnetic resonance spectroscopy (2008), Biochemistry, 47, 8950-8960.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
analysis of electron paramagnetic resonance signals for free enzyme, the enzyme-S-adenosyl-L-methionine complex, and a rhombic signal presumably derived from the enzyme-S-adenosyl-L-methionine-substrate ternary complex show close interaction of both S-adenosyl-L-methione and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
S-adenosyl-L-methionine pH 8.0, 28┬░C, EDTA-treated enzyme Niallia circulans

Metals/Ions

Metals/Ions Comment Organism Structure
Iron close interaction of both S-adenosyl-L-methionine and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans Q8G907
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine radical S-adenosyl-L-methionine enzyme. Ketone formation is the rate-determining step in this reaction. Reaction involves formation of a 2-deoxy-scyllo-inosamine radical which can easily be converted to a highly reductive ketyl radical by deprotonation through proton-coupled electron transfer Niallia circulans 3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine
-
?

Synonyms

Synonyms Comment Organism
BtrN
-
Niallia circulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.021
-
S-adenosyl-L-methionine pH 8.0, 28┬░C, EDTA-treated enzyme Niallia circulans

Cofactor

Cofactor Comment Organism Structure
Fe-S center one [4Fe-4S] cluster per monomer, close interaction of both S-adenosyl-L-methione and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster Niallia circulans